食品与发酵工业 >

2024 , Vol. 50 >Issue 11: 27 - 37

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.035686

研究报告

草黄口蘑多糖对S180荷瘤小鼠抗肿瘤活性分子机制研究

  • 赵倪慧 ,
  • 蒋琳 ,
  • 张丁 ,
  • 段江波 ,
  • 丁祥 ,
  • 侯怡铃
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  • 1(西华师范大学 生命科学学院,西南野生动植物资源保护教育部重点实验室,四川 南充,637009)
    2(西华师范大学 环境科学与工程学院,四川 南充,637009)
第一作者:硕士研究生(侯怡铃教授为通信作者,E-mail:starthlh@126.com)

收稿日期: 2023-03-31

  修回日期: 2023-05-22

  网络出版日期: 2024-07-12

基金资助

四川省科技厅应用基础面上项目(2022NSFSC0107);四川省科技厅农业科技成果转化资金项目(2022NZZJ0003);四川省转移支付科技成果转移转化示范项目(22ZYZFSF0009;23ZHSF0082);农业农村部农业微生物资源收集与保藏重点实验室开放课题(KLMRCP2021-01)

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Molecular mechanism of anti-tumor activity of polysaccharide from Tricholoma lascivum (Fr.) Gillet on S180 tumor-bearing mice

  • ZHAO Nihui ,
  • JIANG Lin ,
  • ZHANG Ding ,
  • DUAN Jiangbo ,
  • DING Xiang ,
  • HOU Yiling
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  • 1(Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), College of Life Sciences, China West Normal University, Nanchong 637009, China)
    2(College of Environmental Science and Engineering, China West Normal University, Nanchong 637009, China)

Received date: 2023-03-31

  Revised date: 2023-05-22

  Online published: 2024-07-12

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摘要

多糖作为口蘑属真菌的主要功能成分之一,具有免疫调节、抗氧化及抗肿瘤等活性。为明确草黄口蘑多糖的结构特征及其对S180荷瘤小鼠抗肿瘤活性相关分子机制,该研究以草黄口蘑子实体经热水浸提法提取的草黄口蘑多糖[Tricholoma lascivum (Fr.) Gillet polysaccharide,TLG-1]为实验材料,初步解析TLG-1结构,以S180荷瘤小鼠为模型检测体内抗肿瘤活性,并采用转录组测序技术对S180肿瘤组织总RNA进行测序分析。结果表明,TLG-1重均分子质量为13 442 Da,由木糖、甘露糖、葡萄糖和半乳糖4种单糖组成,其比例为1.57∶1.45∶2.84∶4.14。TLG-1可显著抑制荷瘤小鼠S180肿瘤的生长(P<0.01),抑瘤率为53.8%。转录组测序分析显示,TLG-1能显著上调mt-Co1、Prlr、Foxa1以及下调Pitx2、Rplp1、Eef1a1及Lgals1等基因,影响S180肿瘤细胞的代谢途径,调控细胞周期并抑制上皮间质转化过程。基因本体富集分析表明,TLG-1可激活细胞因子介导的信号通路,引发细胞免疫。京都基因与基因组百科全书富集分析显示,在Jak-STAT信号通路中的Il21、Il12rb2、Il12b、Il2ra、Il2rb、Il2rg、Il15ra和Ifng等基因显著上调,通过转换肿瘤相关巨噬细胞表型及分泌干扰素-γ等,增强小鼠抗肿瘤免疫应答。该研究结果为草黄口蘑多糖的开发和利用提供了一定的理论基础。

关键词： 草黄口蘑多糖TLG-1; 抗肿瘤; S180; 转录组测序技术; Jak-STAT信号通路

本文引用格式

赵倪慧 , 蒋琳 , 张丁 , 段江波 , 丁祥 , 侯怡铃 . 草黄口蘑多糖对S180荷瘤小鼠抗肿瘤活性分子机制研究[J]. 食品与发酵工业, 2024 , 50(11) : 27 -37 . DOI: 10.13995/j.cnki.11-1802/ts.035686

Abstract

As one of the main functional components of Tricholoma, polysaccharides have activities such as immune regulation, antioxidant and anti-tumor effects.To clarify the structure and the molecular mechanisms of anti-tumor activity of polysaccharide from Tricholoma lascivum (Fr.) Gillet on S180 tumor-bearing mice, the Tricholoma lascivum (Fr.) Gillet polysaccharide (TLG-1) extracted from the fruiting body of Tricholoma lascivum (Fr.) Gillet by hot water extraction method was used as the experimental material, the structure of TLG-1 was preliminarily analyzed, S180 tumor bearing-mice were used as models to detect anti-tumor activity in vivo, the total RNA of S180 tumor tissue was also sequenced using RNA sequencing.Results showed that TLG-1 had the weight-average molecular weight of 13 442 Da and was composed of xylose, mannose, glucose, and galactose in a ratio of 1.57∶1.45∶2.84∶4.14.TLG-1 could significantly inhibit the growth of S180 tumors in mice (P<0.01), with a tumor inhibition rate of 53.8%.Transcriptome sequencing analysis showed that TLG-1 could significantly up-regulate genes such as mt-Co1, Prlr, Foxa1, and down-regulate genes such as Pitx2, Rplp1, Eef1a1, and Lgals1, affecting the metabolic pathways of S180 tumor cells, regulating the cell cycle, and inhibiting epithelial-mesenchymal transition process.Gene ontology enrichment analysis showed that TLG-1 could activate cytokine-mediated signaling pathways and trigger cellular immunity.Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that genes such as Il21, Il12rb2, Il12b, Il2ra, Il2rb, Il2rg, Il15ra, and Ifng in the Jak-STAT signaling pathway were significantly up-regulated.TLG-1 could enhance the anti-tumor immune response of mice by transforming tumor-associated macrophage phenotype, and secreting interferon-γ.The results could provide a theoretical basis for the development and utilization of TLG-1.

Key words： polysaccharide TLG-1; anti-tumor; S180; RNA sequencing; Jak STAT signaling pathway

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