Lycium ruthenicum Murr., a medicinal and edible plant with good efficacy, has attracted more and more interest as human beings pay more and more attention to health and health care in the 21 st century.It is of great significance to study the chemical constituents of pharmacologically active substances in Lycium ruthenicum Murr., which is beneficial for its further processing, development and utilization in the fields of food industry and pharmaceuticals industry.The aim of this work is to analyze the aroma components and chemical composition of polyphenols in fresh fruit of Lycium ruthenicum Murr.from Jiuquan city, Gansu province.Headspace solid phase microextraction coupled with gas chromatography-mass spectrometry was used to qualitatively and quantitatively analyze the aroma components of fresh fruits of Lycium ruthenicum Murr.The chemical composition of polyphenols in fresh fruits was qualitatively analyzed by ultra-high performance liquid chromatography- electrospray ionization-qudropole time-of-flight mass spectrometry.A total of 63 volatile chemical components, including alcohols, aldehydes, ketones, esters, and hydrocarbons and other types of compounds were identified from the aroma components, accounting for 95.4% of the total aroma components.Among them, 25 compounds were the synthetic flavors for food in the National Standard GB 2760—2014 National Food safety Standard-Standard for the Use of Food Additives.There were 26 kinds of alcohols in the aroma components, and the total content accounted for the highest proportion (44.98%).After extraction and purification by HPD-500 macroporous adsorption resin, 73 polyphenol compounds were qualitatively identified by liquid-mass, which were mainly composed of flavonoids and phenolic acids.In this study, the chemical constituents of the aroma and polyphenols of were analyzed, which lay a foundation for the further study of the medicinal and edible effects of Lycium ruthenicum Murr.
[1] 潘菲, 李玉丽, 李玉林, 等.五产区黑果枸杞化学成分测定及综合比较[J].中华中医药杂志, 2023, 38(11):5474-5478.
PAN F, LI Y L, et al.Determination and comprehensive comparison on chemical constituents of Lycium ruthenicum Murr.from five producing areas[J].China Journal of Traditional Chinese Medicine and Pharmacy,2023, 38(11):5474-5478.
[2] 邢丽杰, 逯霞, 唐宗贵, 等.黑果枸杞籽油品质及体外抗氧化活性[J].中国油脂, 2022, 47(9):90-94.
XING L J, LU X, TANG Z G, et al.Quality and in vitro antioxidant activity of Lycium ruthenicum seed oil[J].China Oils and Fats, 2022, 47(9):90-94.
[3] 马丽娟, 霍鹏超, 孙梦茹, 等.黑果枸杞化学成分和药理活性的研究进展[J].中草药, 2020, 51(22):5884-5893.
MA L J, HUO P C, SUN M R, et al.Research progress on chemical constituents and pharmacological activities of Lycium ruthenicum[J].Chinese Traditional and Herbal Drugs, 2020, 51(22):5884-5893.
[4] 邢丽杰, 王远, 刘帅光, 等.黑果枸杞中活性成分的研究进展[J].农产品加工, 2021(10):66-69;72.
XING L J, WANG Y, LIU S G, et al.Research Progress of Active Ingredients in Lycium ruthenicum Murr.[J].Farm Products Processing, 2021(10):66-69;72.
[5] 陈栋杰, 郭盛, 伊艳玲, 等.枸杞属植物酰胺类成分及其生物活性研究进展[J].中草药, 2023, 54(1):317-333.
CHEN D J, GUO S, YI Y L, et al. Research progress on amides from Lycium and their biological activities[J].Chinese Traditional and Herbal Drugs, 2023, 54(1):317-333.
[6] 苑雪彤, 邱向阳, 刘志惠, 等.酶辅助超声提取黑果枸杞多糖工艺及其保肝活性研究[J].沈阳药科大学学报, 2023, 40(10):1366-1374.
YUAN X T, QIU X Y, LIU Z H, et al.Enzymatic auxiliary ultrasonic extraction of polysaccharide from Lycium ruthenicum Murr. and its hepatoprotective activity[J].Journal of Shenyang Pharmaceutical University, 2023, 40(10):1366-1374.
[7] 张佳琪, 赵英力, 王浩宁, 等.黑果枸杞研究进展及产业发展对策[J].北方园艺, 2020(19):134-140.
ZHANG J Q, ZHAO Y L, WANG H N, et al.Research Progress and Industrial Development Countermeasures of Lycium barbarum[J].Northern Horiticulture, 2020(19):134-140.
[8] 周琪乐, 罗诗萌, 龚凌慧, 等.黑果枸杞风味品质的分析研究[J].食品安全质量检测学报, 2021, 12(21):8381-8389.
ZHOU Q L, LUO S M, GONG L H, et al.Study on flavor quality of Lycium ruthenicum Murr.[J].Journal of Food Safety and Quality, 2021, 12(21):8381-8389.
[9] 彭玉娇, 崔学宇, 邵元元, 等.利用LC-MS技术解析黑果枸杞、红果枸杞代谢物的差异[J].食品与生物技术学报, 2021, 40(9):56-63.
PENG Y J, CUI X Y, SHAO Y Y, et al.Analysis of the Differences of Metabolites between Lycium ruthenicum Murr.and Lycium barbarum L.by LC-MS[J].Journal of Food Science and Biotechnology, 2021, 40(9):56-63.
[10] 王天琦, 马兆成, 吴军民, 等.黑果枸杞中花色苷的高效液相色谱分析研究[J].分析科学学报, 2020, 36(4):465-470.
WANG T Q, MA Z C, WU J M, et al.Analysis of anthocyanins in Lycium ruthenicum by high performance liquid chromatography[J].Journal of Analytical Science, 2020, 36(4):465-470.
[11] 杨理章, 倪德让, 杨玉波, 等.液液微萃取结合气质联用技术分析高温大曲中风味物质[J].中国酿造, 2022, 41(8):223-228.
YANG L Z, NI D R, YANG Y B, et al.Analysis of flavor compounds in high-temperature Daqu by liquid-liquid microextraction combined with gas chromatography-mass spectrometry[J].China Brewing, 2022, 41(8):223-228.
[12] 王春林, 武芸, 王风琴.甘肃民勤野生黑果枸杞果实营养成分分析[J].甘肃农业科技, 2021, 52(6):33-37.
WANG C L, WU Y, WANG F Q, et al.Analysis of nutrient ingredient of wild Lycium ruthenicum from Minqin in Gansu[J].Gansu Agricultural Science and Technology, 2021, 52(6):33-37.
[13] 王春林, 武芸, 芦娅妮, 等.Plackett-Burnman联合响应面法优化黑果枸杞黄酮提取工艺及抗氧化性研究[J].食品工业科技, 2021, 42(18):218-225.
WANG C L, WU Y, LU Y N, et al.Optimization of extraction process of flavonoids from Lycium ruthenicum Murr.by plackett-burnman with response surface methodology and its antioxidation activity[J].Science and Technology of Food Industry, 2021, 42(18):218-225.
[14] 武芸, 王春林, 王丽朋, 等.黑果枸杞多酚吸附分离特性及抗氧化性研究[J].食品与发酵工业, 2021, 47(2):70-77.
WU Y, L WANG C L, WANG L P, et al. Lycium ruthenicum Murr.polyphenols adsorption separation properties and oxidation resistance[J].Food and Fermentation Industries, 2021, 47(2):70-77.
[15] 兰天, 段国珍, 祁有朝, 等.黑果枸杞和黄果枸杞的广泛靶向代谢组学分析[J].食品科学, 2023.http://kns.cnki.net/kcms/detail/11.2206.TS.20231020.1806.072.html.
LAN T, DUAN G C, QI Y C, et al.Widely targeted metabolomic analysis of yellow wolfberry and black wolfberry[J].Food Science, 2023.http://kns.cnki.net/kcms/detail/11.2206.TS.20231020.1806.072.html.
[16] 王琪, 唐惠儒.黑果枸杞和宁夏枸杞果实中多酚类物质组成的差异研究[J].上海交通大学学报(医学版), 2019, 39(1):39-46.
WANG Q, TANG H R.Composition differences of polyphenolic compounds in fruits of Lycium ruthenicum Murr.and Lycium barbarum L[J].Journal of Shanghai Jiao Tong University (Medical Science), 2019, 39(1):39-46.
[17] 闫亚美, 米佳, 禄璐, 等.不同枸杞叶茶中黄酮、多酚及氨基酸组成分析[J].食品研究与开发, 2020, 41(23):27-31.
YAN Y M, MI Q, LU L, et al.Analysis of flavones, polyphenols and amino acids in tea of leaves from different wolfberry[J].Food Research and Development, 2020, 41(23):27-31.