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玉米赤霉烯酮生物脱毒及降解酶分子改造研究进展

  • 李晨亮 ,
  • 孙长坡 ,
  • 管骁 ,
  • 常晓娇
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  • 1(上海理工大学 健康科学与工程学院,上海,200093)
    2(国家粮食和物资储备局科学研究院,北京,100037)
    3(国家粮食和物资储备局标准质量中心,北京,100834)
第一作者:硕士研究生(常晓娇副研究员为通信作者,E-mail:cxj@ags.ac.cn)

收稿日期: 2023-11-03

  修回日期: 2023-11-23

  网络出版日期: 2024-09-19

基金资助

国家重点研发计划(2023YFF1104604);国家粮食和物资储备局科学研究院专项课题(JY2205)

Research progress on zearalenone biodetoxification and molecular modification of degrading enzymes

  • LI Chenliang ,
  • SUN Changpo ,
  • GUAN Xiao ,
  • CHANG Xiaojiao
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  • 1(School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China)
    2(Academy of National Food and Strategic Reserves Administration, Beijing 100037, China)
    3(Standard and Quality Center of National Food and Strategic Reserves Administration, Beijing 100834, China)

Received date: 2023-11-03

  Revised date: 2023-11-23

  Online published: 2024-09-19

摘要

玉米赤霉烯酮(zearalenone,ZEN)是由镰刀菌(Fusarium)产生的真菌毒素,严重威胁人畜健康。生物降解作为一种靶向性强、绿色、安全的脱毒方法受到广泛关注,但因稳定性和催化效率等问题限制了其在工业上的应用。近年来,随着计算生物学的不断发展,人们以蛋白质分子设计和结构模拟为基础在提高酶活力和稳定性,以及优化底物选择性等方面取得了一些进展。该文系统综述了ZEN降解酶的挖掘及生物催化机理,总结了基于蛋白质结构设计策略提高ZEN降解酶性能的研究进展,旨在为工业化ZEN降解酶制剂的开发及粮油食品中真菌毒素的系统控制提供指导。

本文引用格式

李晨亮 , 孙长坡 , 管骁 , 常晓娇 . 玉米赤霉烯酮生物脱毒及降解酶分子改造研究进展[J]. 食品与发酵工业, 2024 , 50(16) : 367 -377 . DOI: 10.13995/j.cnki.11-1802/ts.037850

Abstract

Zearalenone (ZEN) is a mycotoxin produced by Fusarium, posing serious threats to human and animal health.Biodegradation, as an environmentally friendly and safe method for ZEN detoxification, has garnered significant attention.However, the application remains limited due to issues such as stability and catalytic efficiency in the industry.In recent years, advancements in computational biology have led to progress in enhancing enzyme activity and stability, as well as optimizing substrate selectivity through protein molecular design and structure simulation.Herein, this study provided a comprehensive review of the discovery and biocatalytic mechanism of ZEN-degrading enzymes and summarized research advancements in improving the performance of these enzymes based on strategies involving protein structure design, offering guidance for the development of industrial preparations containing ZEN-degrading enzymes and the systematical control of mycotoxins contaminated in grain and oil.

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