研究报告

超声联合酶解处理对发酵整果枸杞子品质影响

  • 陈君然 ,
  • 李岢祎 ,
  • 李海川 ,
  • 白亚辉 ,
  • 胡云峰 ,
  • 马云 ,
  • 段亚宗
展开
  • 1(天津科技大学 食品科学与工程学院,天津, 300457)
    2(早康枸杞股份有限公司,宁夏 中卫,751700)
    3(天津市野森果树种植专业合作社,天津, 301913)
第一作者:博士,讲师(胡云峰研究员为通信作者,E-mail:hu-yf@163.com)

收稿日期: 2023-04-17

  修回日期: 2023-06-03

  网络出版日期: 2024-10-14

基金资助

天津市林果现代农业产业技术体系项目(ITTHRS2021000);天津市科技支撑重点项目(20YFZCSN00980)

Quality evaluation of lactic acid bacteria fermented Lycium barbarum

  • CHEN Junran ,
  • LI Keyi ,
  • LI Haichuan ,
  • BAI Yahui ,
  • HU Yunfeng ,
  • MA Yun ,
  • DUAN Yazong
Expand
  • 1(College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China)
    2(Zaokang Gojiberry Inc., Zhongwei 751700, China)
    3(Tianjin Yesen Fruit Tree Planting Professional Cooperative, Tianjin 301913, China)

Received date: 2023-04-17

  Revised date: 2023-06-03

  Online published: 2024-10-14

摘要

为充分开发利用枸杞子资源,该研究以整果枸杞子为原料,采用乳酸菌对其进行发酵,同时为了缩短发酵时间、改善枸杞子的功能性和风味,采用超声联合酶解处理技术对枸杞子进行发酵预处理,并对其品质指标进行检测。结果表明,枸杞子经发酵后总酚、总黄酮含量及抗氧化能力得到了明显提升;超声联合酶解处理进一步增强了发酵强度、活菌数和抗氧化能力。主成分分析综合评价函数表明,超声联合酶解预处理后枸杞子品质最佳,结合正交偏最小二乘判别分析确定活菌数及还原糖含量为超声联合酶解处理对发酵整果枸杞子品质影响的差异指标。超声联合酶解处理后的发酵枸杞子挥发性风味物质种类和含量均有所提升,其中烯烃类化合物相对含量达到了32.64%,赋予其柠檬、薄荷似偏向清新风味,丰富了枸杞子的风味层次。

本文引用格式

陈君然 , 李岢祎 , 李海川 , 白亚辉 , 胡云峰 , 马云 , 段亚宗 . 超声联合酶解处理对发酵整果枸杞子品质影响[J]. 食品与发酵工业, 2024 , 50(18) : 201 -208 . DOI: 10.13995/j.cnki.11-1802/ts.035853

Abstract

To fully exploit the resources of Lycium barbarum, this study investigated the fermentation of Lycium barbarum using whole fruit as raw material and lactic acid bacteria.Ultrasonic combined with enzymatic treatment technology was used as pretreatment to shorten fermentation time and improve the functionality and flavor of Lycium barbarum, and the quality indexes were tested.The results showed that the total phenols, total flavonoids, and antioxidant capacity of Lycium barbarum were significantly improved after fermentation, and the ultrasonic combined enzymatic treatment further enhanced the fermentation strength, viable bacterial count, and antioxidant capacity.The comprehensive evaluation function of the principal component analysis showed that the quality of Lycium barbarum was best after the combined enzymatic treatment, and the orthogonal partial least squares discriminant analysis identified the number of viable bacteria and reducing sugars as the difference indicators of the effect of the combined enzymatic pretreatment on the quality of fermented whole Lycium barbarum.The volatile flavor species and content of the fermented Lycium barbarum were increased after the pretreatment, and the relative content of olefins reached 32.64%, which gave it a lemon-like and mint-like fresh flavor, masked the irritating odor to a certain extent, and enriched the flavor level of the Lycium barbarum.

参考文献

[1] DONNO D, BECCARO G L, MELLANO M G, et al. Goji berry fruit (Lycium spp.): Antioxidant compound fingerprint and bioactivity evaluation[J]. Journal of Functional Foods, 2015, 18:1070-1085.
[2] FENG L, TANG N C, LIU R J, et al. Effects of different processing methods on bioactive substances and antioxidation properties of Lycium barbarum (goji berry) from China[J]. Food Bioscience, 2021, 42:101048.
[3] GAO H, WEN J J, HU J L, et al. Momordica charantia juice with Lactobacillus plantarum fermentation: Chemical composition, antioxidant properties and aroma profile[J]. Food Bioscience, 2019, 29:62-72.
[4] HU X, ZENG J R, SHEN F, et al. Citrus pomace fermentation with autochthonous probiotics improves its nutrient composition and antioxidant activities[J]. LWT, 2022, 157:113076.
[5] 郭波, 梁思宇, 陈楚坚, 等. 青梅果酒整果发酵工艺的研究[J]. 酿酒科技, 2013(8):79-81.
GUO B, LIANG S Y, CHEN C J, et al. Study on the production of green plum wine[J]. Liquor-Making Science & Technology, 2013(8):79-81.
[6] 胡来丽. 不同加工工艺中百香果生理活性物质量变规律研究[D]. 贵阳: 贵州大学, 2022.
HU L L. Study on quantitative change law of physiological active substances of passion fruit in different processing technologies[D]. Guiyang: Guizhou University, 2022.
[7] YU Z, SU Y, ZHANG Y L, et al. Potential use of ultrasound to promote fermentation, maturation, and properties of fermented foods: A review[J]. Food Chemistry, 2021, 357:129805.
[8] 颉向红. 酶解结合多菌种对枸杞饮料发酵过程中发酵特性的影响研究[D]. 银川: 宁夏大学, 2019.
XIE X H. Effect of enzymatic hydrolysis combined with multi-strains on fermentation characteristics of Lycium barbarum beverage during fermentation[D]. Yinchuan: Ningxia University, 2019.
[9] TIEN T N, NGUYEN T C, NGUYEN C N, et al. Protease increases ethanol yield and decreases fermentation time in no-cook process during very-high-gravity ethanol production from rice[J]. Process Biochemistry, 2022, 117:10-18.
[10] 李婷婷. 超声波催陈对镇江香醋品质的影响[D]. 镇江: 江苏大学, 2018.
LI T T. Effect of ultrasonic aging on the quality of Zhenjiang aromatic vinegar[D]. Zhenjiang: Jiangsu University, 2018.
[11] 彭雨露. 乳酸菌协同酶解发酵制备稀奶油-大豆分离蛋白复合风味香基的研究[D]. 无锡: 江南大学, 2022.
PENG Y L. Study on preparation of compound flavor aroma base of cream-soy protein isolate by lactic acid bacteria synergistic enzymatic fermentation[D]. Wuxi: Jiangnan University, 2022.
[12] 王娜. 枸杞熟化过程中美拉德反应的研究[D]. 天津: 天津科技大学, 2018.
WANG N. Study on Maillard reaction during the ripening of Lycium barbarum L.[D]. Tianjin: Tianjin University of Science & Technology, 2018.
[13] 李佩佩, 颉向红, 王聪, 等. 不同发酵方式下枸杞饮料主要成分及其抗氧化活性[J]. 食品与发酵工业, 2019, 45(24):90-97.
LI P P, XIE X H, WANG C, et al. Main components and antioxidant activities of Lycium barbarum L. beverages using two different fermentation processes[J]. Food and Fermentation Industries, 2019, 45(24):90-97.
[14] 张玉芝, 赵丹, 王冬冬, 等. 枸杞枝发酵液中黄酮类成分的活性研究[J]. 日用化学工业, 2022, 52(4):390-395.
ZHANG Y Z, ZHAO D, WANG D D, et al. Study on the activity of flavonoids in the fermentation broth of Chinese wolfberry branches[J]. China Surfactant Detergent & Cosmetics, 2022, 52(4):390-395.
[15] 高庆超. 黑果枸杞酵素发酵过程中微生物及物质变化规律的研究[D]. 西宁: 青海大学, 2020.
GAO Q C. Study on the change law of microorganisms and substances in the process of enzymatic fermentation of Lycium barbarum L.[D]. Xining: Qinghai University, 2020.
[16] 李杰, 赵声兰, 陈朝银. 核桃青皮果蔬酵素的成分组成及体外抗氧化活性研究[J]. 食品工业科技, 2016, 37(10):117-122.
LI J, ZHAO S L, CHEN C Y. Chemical composition and in vitro antioxidant properties of walnut green husk fruit and vegetable compound ferment[J]. Science and Technology of Food Industry, 2016, 37(10):117-122.
[17] 熊巧仪, 刘丁群, 鲁敏. 刺梨自然发酵后抗氧化物质含量与抗氧化活性分析[J]. 食品研究与开发, 2018, 39(1):147-151.
XIONG Q Y, LIU D Q, LU M. Analysis in content of antioxidant substances and antioxidant activity after natural fermentation of Rosa roxburghii fruits[J]. Food Research and Development, 2018, 39(1):147-151.
[18] 周艳平, 张彩猛, 孔祥珍, 等. 酸奶发酵剂对大豆酸奶品质的影响[J]. 大豆科学, 2018, 37(1):149-156.
ZHOU Y P, ZHANG C M, KONG X Z, et al. Effect of yoghurt starter cultures on the quality of soybean yoghurt[J]. Soybean Science, 2018, 37(1):149-156.
[19] 王振斌, 邵淑萍, 赵帅, 等. 主成分分析在超声催陈食醋综合评定中的应用[J]. 食品与生物技术学报, 2015, 34(6):627-633.
WANG Z B, SHAO S P, ZHAO S, et al. Application of principal component analysis in comprehensive assessment of ultrasound treatment on vinegar[J]. Journal of Food Science and Biotechnology, 2015, 34(6):627-633.
[20] LIU F R, CHEN Z X, SHAO J J, et al. Effect of fermentation on the peptide content, phenolics and antioxidant activity of defatted wheat germ[J]. Food Bioscience, 2017, 20:141-148.
[21] HUR S J, LEE S Y, KIM Y C, et al. Effect of fermentation on the antioxidant activity in plant-based foods[J]. Food Chemistry, 2014, 160:346-356.
[22] OJHA K S, MASON T J, O’DONNELL C P, et al. Ultrasound technology for food fermentation applications[J]. Ultrasonics Sonochemistry, 2017, 34:410-417.
[23] 王洋. 超声预处理酶解制备高生物利用度玉米蛋白及其过程近红外光谱原位实时监测[D]. 镇江: 江苏大学, 2020.
WANG Y. Preparation of high bioavailability corn protein by ultrasonic pretreatment and enzymatic hydrolysis and its in-situ real-time monitoring by near infrared spectroscopy[D]. Zhenjiang: Jiangsu University, 2020.
[24] 任文斌. 超声辅助酿酒酵母乙醇发酵及其作用机制的研究[D]. 镇江: 江苏大学, 2021.
REN W B. Study on ultrasonic-assisted ethanol fermentation of Saccharomyces cerevisiae and its mechanism[D]. Zhenjiang: Jiangsu University, 2021.
[25] TIWARI B K, PATRAS A, BRUNTON N, et al. Effect of ultrasound processing on anthocyanins and color of red grape juice[J]. Ultrasonics Sonochemistry, 2010, 17(3):598-604.
[26] 马亚琴, 张晨, 邓涂静, 等. 水果及其加工产品中糖苷键合态香气物质的研究进展[J]. 食品与发酵工业, 2022, 48(6):290-298.
MA Y Q, ZHANG C, DENG T J, et al. Research progress of glycoside bonded aroma compounds in fruits and their processed products[J]. Food and Fermentation Industries, 2022, 48(6):290-298.
[27] DU TOIT S C, ROSSOUW D, DU TOIT M, et al. Enforced mutualism leads to improved cooperative behavior between Saccharomyces cerevisiae and Lactobacillus plantarum[J]. Microorganisms, 2020, 8(8):1109.
[28] 张浩然. 沙果醋混菌发酵工艺及超声催陈研究[D]. 北京: 北京林业大学, 2020.
ZHANG H R. Study on mixed fermentation technology and ultrasonic aging of sand fruit vinegar[D]. Beijing: Beijing Forestry University, 2020.
[29] KONG C L, ZHU D Y, ZHAO Y, et al. Spent yeast polysaccharides in mixed alcoholic fermentation between Pichia kluyveri, Pichia fermentans and Saccharomyces cerevisiae retarded wine fruity ester hydrolysis[J]. Journal of Food Composition and Analysis, 2022, 105:104200.
[30] 郭静. 猕猴桃果实及果酒香气成分研究[D]. 杨凌: 西北农林科技大学, 2007.
GUO J. Study on aroma components of kiwifruit and fruit wine[D]. Yangling: Northwest A & F University, 2007.
文章导航

/