食品与发酵工业 >

2024 , Vol. 50 >Issue 18: 265 - 272

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.037594

研究报告

深共熔溶剂提取酒糟多酚类物质及其体外抗氧化、降血糖活性研究

  • 晏俊玲 ,
  • 李茂 ,
  • 张伟建 ,
  • 赵佳伟 ,
  • 覃凤阳 ,
  • 赵东 ,
  • 郑佳
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  • 1(宜宾五粮液股份有限公司技术研究中心,四川 宜宾,644007)
    2(中国轻工业浓香型白酒固态发酵重点实验室,四川 宜宾,644000)
第一作者：硕士,助理工程师(郑佳高级工程师为通信作者,E-mail:1742865041@qq.com)

收稿日期: 2023-10-09

  修回日期: 2023-11-02

  网络出版日期: 2024-10-14

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Deep eutectic solvent extraction of distiller′s grains polyphenols and its antioxidant and hypoglycemic abilities in vitro

  • YAN Junling ,
  • LI Mao ,
  • ZHANG Weijian ,
  • ZHAO Jiawei ,
  • QIN Fengyang ,
  • ZHAO Dong ,
  • ZHENG Jia
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  • 1(Technology Research Center of Wuliangye Distillery Co.Ltd., Yibin 644007, China)
    2(Key Laboratory of Wuliangye-flavor Liquor Solid-state Fermentation, China National Light Industry, Yibin 644000, China)

Received date: 2023-10-09

  Revised date: 2023-11-02

  Online published: 2024-10-14

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摘要

为了充分利用酒糟中多酚类活性成分资源,首先从10种深共熔溶剂中筛选最适宜酒糟多酚类物质提取的体系组合,采用响应曲面法优化深共熔溶剂提取酒糟多酚的工艺,运用高效液相色谱分析酒糟多酚类物质,并评估酒糟多酚体外抗氧化和降血糖活性。结果表明,酒糟多酚最佳的深共熔溶剂体系为氯化胆碱-果糖,最佳提取工艺为深共熔溶剂含水率41%,提取时间31 min,液料比31∶1(mL∶g),提取温度40 ℃,在此条件下酒糟多酚含量为(6.28±0.18) mg GAE/g。并且在检测的酚类物质中,对香豆酸含量(466.7±0.20) μg/g最高,占7种酚类物质的30%左右。酒糟多酚清除羟自由基、DPPH自由基和ABTS阳离子自由基的IC50值分别为0.886、0.291、0.302 mg/mL,结合抑制α-淀粉酶和α-葡萄糖苷酶活力的IC50值分别为0.938和2.329 mg/mL,表明酒糟多酚具有较强抗氧化和降血糖能力。深共熔溶剂提取酒糟多酚切实可行,并且酒糟多酚提取物具有较强的体外抗氧化和降血糖活性。

关键词： 深共熔溶剂; 酒糟; 多酚; 抗氧化; 降血糖

本文引用格式

晏俊玲 , 李茂 , 张伟建 , 赵佳伟 , 覃凤阳 , 赵东 , 郑佳 . 深共熔溶剂提取酒糟多酚类物质及其体外抗氧化、降血糖活性研究[J]. 食品与发酵工业, 2024 , 50(18) : 265 -272 . DOI: 10.13995/j.cnki.11-1802/ts.037594

Abstract

To utilize distiller′s grains polyphenols (DGPs) resources, ten kinds of deep eutectic solvents (DESs) were first applied to extract DGPs, the extraction conditions were optimized by response surface methodology, the polyphenols were analyzed by high performance liquid chromatography, and their antioxidant and hypoglycemic activities in vitro were analyzed.Results showed that the DES composed of Choline chloride and fructose was the most suitable for DGPs extraction.The optimum extraction process was as follows, the water content of DES was 41%, the ratio of liquid-solid was 31∶1 (mL∶g), at 40 ℃ extracting for 31 min, the content of DGPs was (6.28±0.18) mg GAE/g.The content of p-coumaric acid (466.7±0.20) μg/g was the highest among the detected phenolic substances, accounting for about 30% of the seven phenolic substances.Meanwhile, The IC50 values of scavenging ·OH, DPPH free radicals, and ABTS cationic radicals were 0.886, 0.291, and 0.302 mg/mL, and the IC50values of α-amylase and α-glucosidase inhibition activity were 0.938 mg/mL and 2.239 mg/mL, respectively, indicating that the DGPs had a strong antioxidant and hypoglycemic ability.In conclusion, the extraction process of DGPs with DES was feasible, and DGPs had certain antioxidant and anti-inflammatory activity in vitro.

Key words： deep eutectic solvent; distiller′s grains; polyphenols; antioxidant; hypoglycemic

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