肠炎沙门氏菌是一种重要的食源性致病菌,多重耐药以及高致病性菌株的出现对临床感染及治疗造成严重负担。为了探究临床分离肠炎沙门氏菌菌株的遗传进化关系及其耐药性和毒力特征,该研究对5株临床来源的肠炎沙门氏菌进行了全基因组测序(whole genome sequencing, WGS),并对其抗生素耐药性和毒力进行了表征。基于WGS生物信息学分析表明5株沙门氏菌均为肠炎血清型,ST型为ST11。cgSNP系统发育分析表明,5株肠炎沙门氏菌中菌株48A和公共数据库中一株分离自鸡的菌株聚类在一起,其余4株菌与公共数据库中的临床分离株有较近的进化关系。微量肉汤稀释法测定结果表明,5株菌株均为抗生素耐药菌株,其中抗4株菌为多重耐药菌,并且菌株33A对黏菌素和多黏菌素B具有耐药性。运动能力、生物被膜形成能力和对大蜡螟幼虫的致死率均表明菌株48A的毒力最强,但该菌株对Caco-2细胞的黏附和侵袭能力并不强。研究结果表明,肠炎沙门氏菌48A是一株高毒力菌株且可能是食品来源,菌株33A具有多黏菌素耐药性,应引起重视。该研究结果可为临床耐药肠炎沙门氏菌的溯源及治疗提供参考。
Salmonella Enteritidis, an important foodborne pathogen, has posed a significant burden on clinical infection treatment due to the emergence of multidrug-resistant and highly pathogenic strains.In order to explore genetic evolutionary relationships among clinical isolates of S. Enteritidis, as well as their antibiotic resistance and virulence characteristics, this study conducted Whole Genome Sequencing (WGS) on five clinical strains, and their antibiotic resistance and virulence were characterized.Bioinformatic analysis of the WGS revealed that the five Salmonella strains belonged to the serotype of Enteritidis, the sequence type (ST) was identified as ST11.The cgSNP phylogenetic analysis indicated that the strain 48A clustered together with one strain isolated from chicken in the public database, while the other four strains showed a closer evolutionary relationship with clinically isolated strains from the database.Microbroth dilution assays confirmed antibiotic resistance of all five strains, with four of them exhibiting multidrug resistance.Strain 33A showed resistance to colistin and polymyxin B.Evaluation of motility, biofilm formation, and lethality to Galleria mellonella larvae revealed that strain 48A exhibited the highest virulence.However, this strain showed relatively weak adhesion and invasion capabilities to Caco-2 cells.The results suggested that strain 48A is a highly virulent strain, potentially originating from food, the resistance to multiple polysaccharides of strain 33A warrants attention.The findings of this study may contribute to the traceability and treatment of clinically resistant S.Enteritidis.
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