该研究旨在探索富含sn-2位上α-亚麻酸的酶法酯交换二元油对自发性高血压大鼠(spontaneously hypertensive rat,SHR)类固醇激素分泌代谢的调控机理,为评价该二元油生理功效并提供数据基础。实验选用7周龄SHR,分为空白、二元油、羊尾油脂、亚麻籽油和菜籽油组,添加10%不同油脂饲料饲喂3个月。ELISA法测定血脂、肝激酶和性激素含量;q-PCR和Western-blot法检测类固醇激素相关基因和蛋白质表达量。结果显示,与空白组比较,二元油组大鼠血中低密度脂蛋白胆固醇降低、高密度脂蛋白胆固醇提高(P>0.05),肝激酶无显著性差异(P>0.05);睾酮水平高于菜籽油组,无显著性差异(P>0.05);二元油组和羊尾油脂组类固醇激素合成急性调节蛋白(synthesis acute regulatory protein,StAR)、胆固醇侧链裂解酶(CYP11A1)和细胞色素P450 17A(CYP17A1)等基因和蛋白质表达显著高于菜籽油组(P<0.05)。由此,可以得出酶法酯交换二元油与其原料羊尾油脂相似,通过改善血脂和肝机能缓解SHR的病症,从而提高睾酮分泌,对其类固醇激素代谢具有有益影响。
侯佳乐
,
包音都古荣·金花
,
迟源
,
马丽燕
,
刘玉滢
,
李雅雯
,
萨如拉
,
王越男
,
呼格吉勒图
,
侯荣伦
,
周欢敏
. 富含sn-2位上α-亚麻酸的酶法酯交换二元油影响自发性高血压大鼠类固醇激素代谢的探索[J]. 食品与发酵工业, 2024
, 50(19)
: 33
-39
.
DOI: 10.13995/j.cnki.11-1802/ts.037881
The objective of this study was to investigate the regulatory mechanism of α-linolenic acid-rich enzyme-esterified oil on steroid hormone secretion and metabolism in spontaneously hypertensive rat (SHR), aiming to provide a data basis for evaluating the physiological efficacy of the enzyme-esterified oil.Seven-week-old SHR were divided into five groups:blank group, enzyme-esterified oil group, sheep tail fat group, flaxseed oil group, and rapeseed oil group.They were fed with 10% different oils for three months.The levels of blood lipids, liver kinase, and sex hormones were determined using ELISA;while the expression of steroid hormone-related genes and proteins was detected through q-PCR and Western-blot analysis.The results revealed that compared to the blank group, LDL-C levels decreased in the enzyme-esterified oil group while HDL-C levels increased(P>0.05).There was no significant difference observed in liver kinase activity among all groups (P>0.05).Although testosterone level in the esterified oil group was higher than that in the rapeseed oil group, there was no statistically significant difference (P>0.05).Notably, expression levels of steroid hormone synthesis acute regulatory protein (StAR), cholesterol side chain cleavage enzyme (CYP11A1), and cytochrome P450 17A (CYP17A1) were significantly higher in both esterified oil and sheep tail fat groups compared to the rapeseed oil group (P<0.05).In conclusion, it can be inferred that enzyme-esterified oil is similar to its raw material sheep tail fat as it improves blood lipid profiles and liver function which subsequently enhances testosterone secretion along with its steroid hormone metabolism thereby alleviating symptoms associated with SHR.
[1] 蒋俊.基于体内自组装胶束形成机制的羊脂油对淫羊藿黄酮苷元抗骨质疏松增效机理研究[D].南京:南京中医药大学, 2015.
JIANG J.Study on the synergistic mechanism of sheep fat oil on epimedium flavonoid aglycone against osteoporosis based on the formation mechanism of self-assembled micelles in vivo[D].Nanjing:Nanjing University of Chinese Medicine, 2015.
[2] 陈玲玲, 贾晓斌, 贾东升.淫羊藿炮制机制研究进展[J].中草药, 2010, 41(12):2108-2111.
CHEN L L, JIA X B, JIA D S.Advances in studies on processing mechanism of Epimedii Folium[J].Chinese Traditional and Herbal Drugs, 2010, 41(12):2108-2111.
[3] AKSU M I.Fatty acid composition of beef intermuscular, sheep tail, beef kidney fats and its effects on shelf life and quality properties of Kavurma[J].Journal of Food Science, 2009, 74(2):S65-S72.
[4] 刘丹, 何鑫, 李涛, 等.不同品种脂臀羊尾脂品质的比较分析[J].现代食品科技, 2019, 35(4):244-249;300.
LIU D, HE X, LI T, et al.Comparative analysis of the quality of different varieties of fat buttocks[J].Modern Food Science and Technology, 2019, 35(4):244-249;300.
[5] 李雅珊, 孙华.亚麻成分提取方法及功效研究综述[J].天津科技, 2018, 45(4):53-56;61.
LI Y S, SUN H.A review of studies about function and method of flax extraction[J].Tianjin Science and Technology, 2018, 45(4):53-56;61.
[6] PUNIA S, SANDHU K S, SIROHA A K, et al.Omega 3-metabolism, absorption, bioavailability and health benefits:A review[J].Pharma Nutrition, 2019, 10:100162.
[7] WILLIAMS D, VERGHESE M, WALKER L T, et al.Flax seed oil and flax seed meal reduce the formation of aberrant crypt foci (ACF) in azoxymethane-induced colon cancer in Fisher 344 male rats[J].Food and Chemical Toxicology, 2007, 45(1):153-159.
[8] 中国营养学会.中国居民膳食指南(2022)[M].北京:人民卫生出版社,2022.
Chinese Nutrition Society.Dietary Guidelines for Chinese Residents (2022)[M].Beijing:People's Medical Publishing House, 2022.
[9] 庞邵杰,贾珊珊,张 坚 新时期国民膳食脂质摄入推荐的几点建议[J] 粮油食品科技, 2022, 30(3):1-6.
PANG S J, ZHANG J, JIA S S.Suggestions for Chinese dietary lipids reference intakes in the new period[J].Science and Technology of Cereals, Oils and Foods, 2022, 30(3):1-6.
[10] PALMER N O, BAKOS H W, FULLSTON T, et al.Impact of obesity on male fertility, sperm function and molecular composition[J].Spermatogenesis, 2012, 2(4):253-263.
[11] POGRMIC-MAJKIC K, FA S, SAMARDZIJA D, et al.Atrazine activates multiple signaling pathways enhancing the rapid hCG-induced androgenesis in rat Leydig cells[J].Toxicology, 2016, 368:37-45.
[12] VAN DEN DRIESCHE S, WALKER M, MCKINNELL C, et al.Proposed role for COUP-TFII in regulating fetal Leydig cell steroidogenesis, perturbation of which leads to masculinization disorders in rodents[J].PLoS One, 2012, 7(5):e37064.
[13] BAKHTYUKOV A A, DERKACH K V, DAR'IN D V, et al.Decrease in the basal and luteinizing hormone receptor agonist-stimulated testosterone production in aging male rats[J].Advances in Gerontology, 2019, 9(2):179-185.
[14] YOO D K, LEE S H.Effect of lipopolysaccharide (LPS) exposure on the reproductive organs of immature female rats[J].Development & Reproduction, 2016, 20(2):113-121.
[15] OKUYAMA H, OHARA N, TATEMATSU K, et al.Testosterone-lowering activity of canola and hydrogenated soybean oil in the stroke-prone spontaneously hypertensive rat[J].The Journal of Toxicological Sciences, 2010, 35(5):743-747.
[16] 马渊, 张永祥.下丘脑-垂体-性腺轴与衰老[J].军事医学科学院院刊, 2002, 26(4):311-313.
MA Y, ZHANG Y X.Hypothalamus-pituitary-gonad axis and senescence[J].Military Medical Sciences, 2002, 26(4):311-313.
[17] 许雅丽, 殷莲华.类固醇激素合成急性调节蛋白(StAR)与胆固醇代谢[J].国际病理科学与临床杂志, 2008, 28(4):358-361.
XU Y L, YIN L H.Steroidogenic acute regulatory protein(StAR)and cholesterol metabolism[J].Journal of Clinical and Pathological Research, 2008, 28(4):358-361.
[18] NISHIKAWA M, OHARA N, NAITO Y, et al.Rapeseed (canola) oil aggravates metabolic syndrome-like conditions in male but not in female stroke-prone spontaneously hypertensive rats (SHRSP)[J].Toxicology Reports, 2022, 9:256-268.
