该研究以23株乳酸菌为研究对象,以产双乙酰能力及乙偶姻还原酶、α-乙酰乳酸脱羧酶、双乙酰还原酶活力等指标评价菌株产双乙酰能力;同时结合自溶度、产酸水平等指标筛选适用于奶酪体系的附属发酵剂菌株;并用加速成熟模型验证相关菌株对切达奶酪品质的影响。结果表明,不同附属发酵剂菌株显著影响切达奶酪的风味,但是对切达奶酪成熟期间的基本成分变化、蛋白水解情况和质构变化无显著影响。添加具有高产双乙酰能力菌株CCFM 601的切达奶酪组在成熟过程中生成的双乙酰含量较高、奶香味较强,且随成熟时间延长,双乙酰下降速度较慢。因此在切达奶酪加工中添加高产双乙酰能力的附属发酵剂菌株,可在切达奶酪成熟过程中可赋予其更多奶香味。
裴志雯
,
殷俊玲
,
夏傲喃
,
王明权
,
李梅
,
刘小鸣
,
赵建新
,
张灏
,
陈卫
. 高产双乙酰的附属发酵剂筛选及其对切达奶酪品质的影响[J]. 食品与发酵工业, 2024
, 50(20)
: 1
-8
.
DOI: 10.13995/j.cnki.11-1802/ts.037809
Diacetyl is one of the most important substances in dairy products and contributes to buttery flavor in cheese.Based on the biosynthesis pathway of diacetyl, 23 strains of lactic acid bacteria were screened according to the ability to produce diacetyl, activities of acetoin reductase, α-acetolactate decarboxylase, and diacetyl reductase for adjunct culture, the growth ability in ripening environment of cheddar cheese, and autolysis degree.The impact of the selected adjunct culture on the properties of cheddar cheese was evaluated with a rapid ripening model.The ripening experimental results showed that the addition of adjunct cultures did not result in significant difference in the physical and chemical indicators, proteolysis, and texture of cheddar cheese except the flavor profile.Compared to the other groups, diacetyl content and buttery flavor was higher in the cheddar cheese prepared with CCFM 601, and the decline of diacetyl was slower during ripening.Therefore, the addition of adjunct cultures in cheddar cheese with high production ability of diacetyl could enhance the milky flavor of cheddar cheese during ripening.
[1] XIANG Q, XIA Y X, SONG J H, et al. Characterization of the key nonvolatile metabolites in Cheddar cheese by partial least squares regression (PLSR), reconstitution, and omission[J]. Food Chemistry, 2023, 403:134034.
[2] 江丽红. 切达奶酪附属发酵剂的筛选及其在促熟中的应用[D]. 无锡: 江南大学, 2014.
JIANG L H. Screening of accessory starter for cheddar cheese and its application in ripening. Wuxi: Jiangnan University, 2014.
[3] 王姣, 许凌云, 张晋华,等. 不同成熟时间切达奶酪中挥发性香气成分及其电子鼻判别分析[J]. 食品科学, 2020, 41(20):175-183.
WANG J, XU L Y, ZHANG J H, et al. Cheddar cheese with different ripening times: Analysis of volatile aroma components and electronic nose discrimination[J]. Food Science, 2020, 41(20):175-183.
[4] 江丽红, 周颖喆, 洪青, 等. 市售陈年切达奶酪风味特征及消费者喜好度研究[J]. 食品工业科技, 2014,35(23): 275-281.
JIANG L H, ZHOU Y Z, HONG Q, et al. Flavor characteristics and consumer preference for the commercial aged Cheddar cheese[J]. Science and Technology of Food Industry, 2014,35(23): 275-281.
[5] DRAKE M A, MCINGVALE S C, GERARD P D, et al. Development of a descriptive language for Cheddar cheese[J]. Journal of Food Science, 2001, 66(9):1422-1427.
[6] WANG J, YANG Z J, WANG Y D,et al. The key aroma compounds and sensory characteristics of commercial Cheddar cheeses[J]. Journal of Dairy Science, 2021, 104(7):7555-7571.
[7] GANESAN B, BROWN K, IRISH D A, et al. Manufacture and sensory analysis of reduced- and low-sodium Cheddar and Mozzarella cheeses[J]. Journal of Dairy Science, 2014, 97(4): 1970-1982.
[8] HOU J, HANNON J A, MCSWEENEY P L H, et al. Effect of curd washing on cheese proteolysis, texture, volatile compounds, and sensory grading in full fat cheddar cheese[J]. International Dairy Journal, 2014, 34(2): 190-198.
[9] 杨铭, 郝晓娜, 罗天淇, 等. 功能基因分析辅助筛选产双乙酰和乙偶姻乳酸菌[J]. 食品科学, 2020, 41(10): 117-123.
YANG M, HAO X N, LUO T Q, et al. Analysis of functional genes related to production of diacetal and acetoin by lactic acid bacteria[J]. Food Science, 2020, 41(10): 117-123.
[10] NICOSIA F D, PINO A, MACIEL G L R, et al. Technological characterization of lactic acid bacteria strains for potential use in cheese manufacture[J].Foods, 2023, 12(6): 1154.
[11] 田玉娟, 周康, 韩新锋, 等. 乳制品中高产双乙酰乳酸菌的筛选及鉴定[J]. 食品科学, 2015, 36(3): 162-165.
TIAN Y J, ZHOU K, HAN X F, et al. Isolation and identification of high-level diacetyl-producing lactic acid bacteria from milk products[J]. Food Science, 2015, 36(3): 162-165.
[12] BETTERA L, LEVANTE A, BANCALARI E, et al. Lacticaseibacillus strains isolated from raw milk: Screening strategy for their qualification as adjunct culture in cheesemaking[J]. Foods, 2023, 12(21): 3949.
[13] AYAD E H E, NASHAT S, EL-SADEK N, et al. Selection of wild lactic acid bacteria isolated from traditional Egyptian dairy products according to production and technological criteria[J]. Food Microbiology, 2004, 21(6): 715-725.
[14] CIOCIA F, MCSWEENEY P L H, PIRAINO P, et al. Use of dairy and non-dairy Lactobacillus plantarum, Lactobacillus paraplantarum and Lactobacillus pentosus strains as adjuncts in cheddar cheese[J]. Dairy Science & Technology, 2013, 93(6):623-640.
[15] 刘婷婷. 预乳化对再制奶酪品质及营养素活性保持研究[D]. 天津: 天津科技大学, 2022.
LIU T T. Study on the effect of pre-emulsification on the quality and nutrient activity of processed cheese[D]. Tianjin: Tianjin University of Science & Technology, 2022.
[16] TAHMAS-KAHYAOĞLU D, CAKMAKCI S, HAYALOGLU A A. Changes during storage in volatile compounds of butter produced using cow, sheep or goat′s milk[J]. Small Ruminant Research, 2022, 211:106691.
