食品与发酵工业 >

2024 , Vol. 50 >Issue 20: 96 - 103

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040156

研究报告

基于生物脱腥的洞庭青鲫鱼汤挥发性成分解析

  • 王伯华 ,
  • 李清钦 ,
  • 郭禹麟 ,
  • 雷颂 ,
  • 罗玉双
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  • 1(湖南文理学院 生命与环境科学学院,湖南 常德,415000)
    2(省部共建淡水鱼类发育生物学国家重点实验室鱼类健康养殖分中心,湖南 常德,415000)
    3(湖南省普通高校3R食品创新创业教育中心,湖南 常德,415000)
第一作者:博士,副教授(通信作者,E-mail:wangbohua0731@126.com)

收稿日期: 2024-06-11

  修回日期: 2024-07-10

  网络出版日期: 2024-11-01

基金资助

国家重点研发计划项目(2023YFD2401604);特色水产品精深加工与开发(企业委托项目)

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Analysis of volatile flavor components in Carassius auratus var.Dongting soup based on biological deodorization

  • WANG Bohua ,
  • LI Qingqin ,
  • GUO Yulin ,
  • LEI Song ,
  • LUO Yushuang
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  • 1(College of Life and Environmental Sciences, Hunan University of Arts and Science, Changde 415000, China)
    2(State Key Laboratory of Development Biology of Freshwater Fish Sub-Center for health aquaculture, Changde 415000, China)
    3(Hunan Provincial 3R Food Innovation and Entrepreneurship Education Center for General Universities, Changde 415000, China)

Received date: 2024-06-11

  Revised date: 2024-07-10

  Online published: 2024-11-01

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摘要

为解析生物脱腥对洞庭青鲫鱼汤主要挥发性物质的影响,明确其生物脱腥机制,结合臭氧预处理,分别用酵母菌和混菌(酵母菌+植物乳杆菌JYLP-002)对其进行生物脱腥。利用顶空固相微萃取结合气质联用仪解析挥发性成分,通过气味活度值和正交偏最小二乘判别分析明确生物脱腥鱼汤的主体风味成分。结果表明,臭氧处理显著降低了已醛、(E)-2-辛烯醛、10-十一烯醛、(E,E)-2,4-癸二烯醛、(E,E)-2,4-庚二烯醛等物质含量,减轻了腥味和油脂味。生物脱腥后,已醛、壬醛、(E,E)-2,4-癸二烯醛、(E,E)-2,4-壬二烯醛等物质经生物转化进一步显著减少,显示出脱腥效果。其中,混菌脱腥处理后产生了已酸乙烯酯、油酸乙酯、亚油酸乙酯等具有花香、果香的优良风味物质,脱腥增香效果明显。

关键词： 洞庭青鲫; 生物脱腥; 挥发性成分; 顶空固相微萃取-气质联用; 正交偏最小二乘判别

本文引用格式

王伯华 , 李清钦 , 郭禹麟 , 雷颂 , 罗玉双 . 基于生物脱腥的洞庭青鲫鱼汤挥发性成分解析[J]. 食品与发酵工业, 2024 , 50(20) : 96 -103 . DOI: 10.13995/j.cnki.11-1802/ts.040156

Abstract

To analyze the effect of biological deodorization on the main volatile components of Carassius auratus var.Dongting soup and clarify the biological deodorization mechanism, yeast fermentation and mixed strains (yeast+Lactobacillus plantarum JYLP-002) fermentation, combined with ozone pretreatment, were used for deodorization.The volatile flavor components were analyzed by headspace-solid phase micro-extraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) and identified by odor activity value (OAV) and orthogonal partial least squares-discriminant analysis (OPLS-DA).Results showed that ozone treatment could significantly reduce the content of hexanal, (E)-2-octenal, 10-undecenal, (E,E)-2,4-decadienal, and (E,E)-2,4-heptandienal, which reducing fishy and greasy odors.The contents of hexanal, nonanal, (E,E)-2,4-decanedienal, (E,E)-2,4-nonadienal, and other odor-producing substances were further significantly reduced by biological deodorization, indicating the efficiency of deodorization.Among them, vinyl caproate, ethyl oleate, ethyl linoleate, and other volatile flavor compounds with pleasant odors such as floral and fruity aroma were produced in mixed strains treated group, which showed better effect of deodorization combined with aroma enhancement.

Key words： Carassius auratus var.Dongting; biological deodorization; volatile compounds; headspace-solid phase micro-extraction-gas chromatography-mass spectrometry; orthogonal partial least squares-discriminant analysis

参考文献

[1] 杨品红,王晓艳,吴维新,等.洞庭青鲫的染色体核型分析及品种鉴定[J].淡水渔业, 2007,37(3):3-7.
YANG P H, WANG X Y, WU W X, et al.Chromosome karyotype analysis of Carassius auratus var.Dongtingking and cultivar identification[J].Freshwater Fisheries, 2007,37(3):3-7.
[2] 刘科均,曾聪,谭俊杰,等.洞庭青鲫的形态和肌肉质构特性[J].湖南农业大学学报(自然科学版), 2019,45(6):650-656.
LIU K J, ZENG C, TAN J J, et al.Characterization of external morphologies and muscle texture of Carassius auratus var.Dongting[J].Journal of Hunan Agricultural University (Natural Sciences),2019,45(6):650-656.
[3] 杨品红,杨利娟,李梦军,等.洞庭青鲫和彭泽鲫DNA提取及其RAPD分析[J].湖南文理学院学报(自然科学版),2007,19(1):59-61.
YANG P H, YANG L J, LI M J, et al.The isolation of DNA and analysis RAPD of Dongtingking crucian carp and Pengze crucian carp[J].Journal of Hunan University of Arts and Science (Natural Science Edition),2007,19(1):59-61.
[4] 王国超,李来好,郝淑贤,等.水产品腥味物质形成机理及相关检测分析技术的研究进展[J].食品工业科技,2012,33(5):401-404;409.
WANG G C, LI L H, HAO S X, et al.Research progress in the mechanism of odor compounds in aquatic product and some relative techniques of detection and analysis[J].Science and Technology of Food Industry,2012,33(5):401-404;409.
[5] WU T L, WANG M Q, WANG P, et al.Advances in the formation and control methods of undesirable flavors in fish[J].Foods,2022,11(16):2504.
[6] 刘利平,李慷,闫莉.水产动物体内土腥味物质的来源、检测及其防控与去除的研究进展[J].水产学报,2021,45(5):813-829.
LIU L P, LI K, YAN L.Sources, determination, prevention and elimination of off-flavour compounds in aquatic animals[J].Journal of Fisheries of China,2021,45(5):813-829.
[7] 赵萍,陈小华,刘俊霞,等.生姜/料酒脱腥过程中大鲵肝挥发性有机物动态变化[J].食品与发酵工业, 2021,47(24):164-172.
ZHAO P, CHEN X H, LIU J X, et al.Dynamic changes of volatile organic compounds in giant salamander (Andrias davidiauns) liver during ginger/cooking wine deodorization[J].Food and Fermentation Industries, 2021,47(24):164-172.
[8] CHEN J, HUANG J, DENG S G, et al.Combining ozone and slurry ice to maximize shelf-life and quality of bighead croaker (Collichthys niveatus)[J].Journal of Food Science and Technology,2016,53(10):3651-3660.
[9] ZHANG T, XUE Y, LI Z J, et al.Effects of ozone on the removal of geosmin and the physicochemical properties of fish meat from bighead carp (Hypophthalmichthys nobilis)[J].Innovative Food Science and Emerging Technologies,2016,34:16-23.
[10] 林晓姿,梁璋成,韦航,等.基于生物脱腥的扇贝挥发性风味成分解析[J].中国食品学报,2022,22(8):261-275.
LIN X Z, LIANG Z C, WEI H, et al.Analysis of volatile flavor components in scallop based on biological deodorization[J].Journal of Chinese Institute of Food Science and Technology,2022,22(8):261-275.
[11] 吴建中,王倩倩,廖顺,等.安琪酵母发酵对秋刀鱼汤的脱腥作用原因分析[J].食品与发酵工业,2019,45(7):213-220.
WU J Z, WANG Q Q, LIAO S, et al.Causes analysis of deodorization of saury soup by Angel yeast fermentation[J].Food and Fermentation Industries, 2019,45(7):213-220.
[12] 黄忠白,明庭红,董丽莎,等.金枪鱼鱼白的植物乳杆菌发酵脱腥增香作用研究[J].中国食品学报,2019,19(2):147-154.
HUANG Z B, MING T H, DONG L S, et al.Studies on the deodorization during fermentation of tuna’s milt by Lactobacillus plantarum[J].Journal of Chinese Institute of Food Science and Technology, 2019,19(2):147-154.
[13] 曾绍东.罗非鱼肉的酶解及产物脱腥技术研究[D].广州:暨南大学,2011.
ZENG S D. Study on enzymatic hydrolysis of Tilapia meat and deodorization technology of its products. Guangzhou: Jinan University, 2011.
[14] 陈珍珍,曾欢,陶宁萍.生物脱腥对银鲫腥味物质及微观结构的影响[J].食品与发酵工业,2023,49(6):126-134.
CHEN Z Z, ZENG H, TAO N P.Effects of biological deodorization on fishy substances and microstructure of Carassius auratus gibelio[J].Food and Fermentation Industries,2023,49(6):126-134.
[15] 王伯华,王美焱,王晨曦,等.臭氧处理对洞庭青鲫鱼肉挥发性成分的影响[J].食品与发酵工业,2023,49(15):288-295.
WANG B H, WANG M Y, WANG C X, et al.Effects of ozone floatation on volatile components of Carassius auratus var.Dongting meat[J].Food and Fermentation Industries,2023,49(15):288-295.
[16] 陈增鑫,刘咏霖,潘芸芸,等.不同脱腥方法对海参肠卵酶解液脱腥效果的比较[J].食品与发酵工业,2022,48(5):187-194.
CHEN Z X, LIU Y L, PAN Y Y, et al.Effect of different deodorization methods on enzymatic hydrolysate of sea cucumber (Stichopus japonicas) intestines and gonads[J].Food and Fermentation Industries,2022,48(5):187-194.
[17] 朱文政,严顺阳,徐艳,等.顶空固相微萃取-气质联用分析不同烹制时间红烧肉挥发性风味成分[J].食品与发酵工业,2021, 47(2):247-253.
ZHU W Z,YAN S Y,XU Y, et al.Analysis of volatile flavor components of braised pork with different cooking time by SPME-GC-MS[J].Food and Fermentation Industries, 2021,47(2):247-253.
[18] 明庭红,裘迪红,周君,等.基于植物乳杆菌发酵草鱼脱腥增香的研究[J].中国食品学报,2017,17(10):202-210.
MING T H, QIU D H, ZHOU J, et al.Analysis of the deodorization and aroma during fermentation of grass carp by Lactobacillus plantarum[J].Journal of Chinese Institute of Food Science and Technology,2017,17(10):202-210.
[19] GÓMEZ GARCÍA-CARPINTERO E, SÁNCHEZ-PALOMO E, GONZÁLEZ VIÑAS M A.Volatile composition of Bobal red wines subjected to alcoholic/malolactic fermentation with oak chips[J].LWT-Food Science and Technology, 2014,55(2):586-594.
[20] 于学萍,童群义.发酵法脱腥对豪猪肝挥发性风味的影响[J].食品与发酵工业,2016,42(2):41-47.
YU X P, TONG Q Y.Effect of fermentation deodorization on volatile compounds of porcupine liver[J].Food and Fermentation Industries,2016,42(2):41-47.
[21] 石建喜,许艳顺,姜启兴,等.混合菌种和盐含量对发酵鲢鱼肉品质的影响研究[J].食品与生物技术学报,2018,37(11):1219-1224.
SHI J X, XU Y S, JIANG Q X, et al.Study on the fermented silver carp quality induced by mixed starter cultures and salt content[J].Journal of Food Science and Biotechnology,2018,37(11):1219-1224.
[22] 曾欢,陶宁萍,张晶晶,等.生物发酵对大口黑鲈的脱腥效果[J].上海海洋大学学报,2021,30(6):1142-1152.
ZENG H, TAO N P, ZHANG J J, et al.Effect of microbial fermentation on removing fishy odors in largemouth bass (Micropterus salmoides) meat[J].Journal of Shanghai Ocean University,2021,30(6):1142-1152.
[23] 里奥·范海默特.化合物嗅觉阈值汇编 (原书第二版)[M].北京:科学出版社,2018.
VAN GEMERT L J.Compilations of Odour Threshold Values in Air, Water and Other Media (second enlarged and revised edition)[M].Beijing:Science Press,2018.
[24] 孙宝国, 陈海涛.食用调香术[M].第3版.北京:化学工业出版社,2016.
SUN B G, CHEN H T.The Technology of Food Flavoring[M].3rd ed.Beijing:Chemical Industry Press,2016.
[25] ZHU H L, LI P, WANG L, et al.Flavor profile of “Dao Ban Xiang” (a traditional dry-cured meat product in Chinese Huizhou cuisine) at different processing stages in winter and summer[J].Food Science & Nutrition, 2023,11(6):2733-2750.
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