食品与发酵工业 >

2024 , Vol. 50 >Issue 20: 113 - 119

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.037815

研究报告

源于传统酿造郫县豆瓣Serratia marcescens GH-2代谢产红色素结构鉴定及其抑菌活性研究

  • 贺紫涵 ,
  • 邹玉婷 ,
  • 许碧涛 ,
  • 商艳玲 ,
  • 张庆
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  • 1(西华大学 食品与生物工程学院,四川 成都,610039)
    2(食品微生物四川省重点实验室,四川 成都,610039)
第一作者:硕士研究生(张庆教授为通信作者,E-mail:biozhangq@163.com)

收稿日期: 2023-10-31

  修回日期: 2024-01-12

  网络出版日期: 2024-11-01

基金资助

四川省自然科学基金项目(2022NSFSC0105)

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Structure identification and antibacterial activity of red pigment metabolized by Serratia marcescens GH-2 from traditionally brewed Pixian Douban

  • HE Zihan ,
  • ZOU Yuting ,
  • XU Bitao ,
  • SHANG Yanling ,
  • ZHANG Qing
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  • 1(College of Food and Bioengineering, Xihua University, Chengdu 610039, China)
    2(Food Microbiology Key Laboratory of Sichuan Province, Chengdu 610039, China)

Received date: 2023-10-31

  Revised date: 2024-01-12

  Online published: 2024-11-01

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摘要

为拓宽产色素微生物资源,以传统酿造郫县豆瓣为分离源,筛选、鉴定具备产红色素能力的菌株。分别采用硅胶柱层析、紫外可见分光光度、高效液相色谱、傅里叶变换红外光谱、核磁共振波谱、高分辨质谱等方法对菌株产红色素进行纯化和结构表征;同时对菌株产红色素的抑菌活性进行初步研究。结果表明,从郫县豆瓣中筛选获得一株产红色素菌株GH-2,结合形态学、生理生化及16S rRNA基因序列分析等鉴定该菌株为粘质沙雷氏菌(Serratia marcescens)。粘质沙雷氏菌GH-2代谢产红色素结构表征鉴定为2-甲基-3-戊基灵菌红素。S.marcescens GH-2产灵菌红素对副溶血性弧菌(Vibrio parahaemolyticus)具有明显的抑制作用,对金黄色葡萄球菌(Staphylococcus aureus)有一定的抑制作用。

关键词： 粘质沙雷氏菌; 分子鉴定; 结构表征; 灵菌红素; 抑菌活性

本文引用格式

贺紫涵 , 邹玉婷 , 许碧涛 , 商艳玲 , 张庆 . 源于传统酿造郫县豆瓣Serratia marcescens GH-2代谢产红色素结构鉴定及其抑菌活性研究[J]. 食品与发酵工业, 2024 , 50(20) : 113 -119 . DOI: 10.13995/j.cnki.11-1802/ts.037815

Abstract

To broaden the resources of pigment-producing microorganisms, strains with the ability to produce red pigment were screened and identified from traditional brewing Pixian Douban.The red pigments were purified and characterized by silica gel column chromatography, ultraviolet visible spectrophotometry (UV-Vis), high performance liquid chromatography (HPLC), fourier transform infrared spectrometer (FTIR), nuclear magnetic resonance (NMR), and high resolution mass spectrum (HRMS).Additionally, the antibacterial activity of the red pigment produced by the strain was preliminarily analyzed.Results showed that a red pigment-producing strain GH-2 was screened from Pixian Douban, and it was identified as Serratia marcescens by morphological, physiological, and biochemical characteristics and 16S rRNA gene sequence analysis.The structure of red pigment produced by S.marcescens GH-2 was identified as 2-methyl-3-pentyl prodigiosin.The prodigiosin produced by S.marcescens GH-2 has a significant inhibitory effect on Vibrio parahaemolyticus and has a certain inhibitory effect on Staphylococcus aureus.

Key words： Serratia marcescens; molecular identification; structural characterization; prodigiosin; antibacterial activity

参考文献

[1] GREWAL J, WOŁA CEWICZ M, PYTER W, et al.Colorful treasure from agro-industrial wastes:A sustainable chassis for microbial pigment production[J].Frontiers in Microbiology, 2022, 13:832918.
[2] VENIL C K, ZAKARIA Z A, AHMAD W A.Bacterial pigments and their applications[J].Process Biochemistry, 2013, 48(7):1065-1079.
[3] QIN Z J, WANG X L, GAO S, et al.Production of natural pigments using microorganisms[J].Journal of Agricultural and Food Chemistry, 2023, 71(24):9243-9254.
[4] ARULDASS C A, DUFOSSÉ L, AHMAD W A.Current perspective of yellowish-orange pigments from microorganisms- a review[J].Journal of Cleaner Production, 2018, 180:168-182.
[5] SAJJAD W, DIN G, RAFIQ M, et al.Pigment production by cold-adapted bacteria and fungi:Colorful tale of cryosphere with wide range applications[J].Extremophiles, 2020, 24(4):447-473.
[6] NIGAM P S, LUKE J S.Food additives:Production of microbial pigments and their antioxidant properties[J].Current Opinion in Food Science, 2016, 7:93-100.
[7] JURIĆ S, JURIĆ M, KRÓL-KILINĆSKA, et al.Sources, stability, encapsulation and application of natural pigments in foods[J].Food Reviews International, 2022, 38(8):1 735-1 790.
[8] 徐春明, 王晓丹, 焦志亮.食用微生物色素的研究进展[J].中国食品添加剂, 2015,26(2):162-168.
XU C M, WANG X D, JIAO Z L.Research progress of edible pigments by microorganisms[J].China Food Additives, 2015,26(2):162-168.
[9] SHEN N, REN J N, LIU Y X, et al.Natural edible pigments:A comprehensive review of resource, chemical classification, biosynthesis pathway, separated methods and application[J].Food Chemistry, 2023, 403:134422.
[10] 于雪, 张威, 吴玉洁, 等.微生物产色素机制及其生物活性[J].微生物学报, 2022, 62(4):1231-1246.
YU X, ZHANG W, WU Y J, et al.Production mechanism and biological activity of microbial pigments[J].Acta Microbiologica Sinica, 2022, 62(4):1231-1246.
[11] 刘洋. 红色素产生菌株的分离鉴定及红色素性质研究[D].黄石:湖北师范大学, 2020.
LIU Y.Isolation and identification of red pigment producing strain and study on the properties of red pigment[D].Huangshi:Hubei Normal University, 2020.
[12] DASGUPTA MANDAL D, MAJUMDAR S.Bacteria as biofactory of pigments:Evolution beyond therapeutics and biotechnological advancements[J].Journal of Bioscience and Bioengineering, 2023, 135(5):349-358.
[13] 鲍奕达, 张丽杰, 郑鹏飞, 等.郫县豆瓣发酵过程的微生物多样性及溯源分析[J].微生物学报, 2020, 60(11):2555-2571.
BAO Y D, ZHANG L J, ZHENG P F, et al.Diversity and source tracking of microbial community in Pixian broad bean paste[J].Acta Microbiologica Sinica, 2020, 60(11):2 555-2 571.
[14] 吉礼, 蒲开阳, 秦覃, 等.农家日晒与阴制郫县豆瓣产品品质对比分析[J].食品与发酵科技, 2022, 58(3):119-123.
JI L, PU K Y, QIN Q, et al.Comparative analysis on the quality of Pixian broad-bean paste made by sunlight and shade in farmhouse[J].Food and Fermentation Science & Technology, 2022, 58(3):119-123.
[15] 李春燕, 牟希, 张媛, 等.1株产灵菌红素家族红色色素细菌的分离鉴定及其色素性质研究[J].食品与发酵工业, 2017, 43(1):1-6.
LI C Y, MOU X, ZHANG Y, et al.The identification of prodigiosin family red pigment producing bacterium and characterization of the red pigment[J].Food and Fermentation Industries, 2017, 43(1):1-6.
[16] 李小群. 海洋细菌Notoacmeibacter sp.BGMRC2072鉴定及其次级代谢产物研究[D].南宁:广西大学, 2018.
LI X Q.Identification of the marine bacterium Notoacmeibacter sp.BGMRC2072 and study on its secondary metabolites[D].Nanning:Guangxi University, 2018.
[17] 王鹏宇, 徐晨琪, 彭涵, 等.一株产灵菌红素菌株黏质沙雷氏菌H04的分离鉴定及发酵条件优化[J].食品与发酵工业, 2023, 49(11):20-26.
WANG P Y, XU C Q, PENG H, et al.Isolation, identification, and optimization of fermentation conditions of a high-yielding prodigiosin Serratia marcescens H04[J].Food and Fermentation Industries, 2023, 49(11):20-26.
[18] RODRÍGUEZ J, LOBATO C, VÁZQUEZ L, et al.Prodigiosin-producing Serratia marcescens as the causal agent of a red colour defect in a blue cheese[J].Foods, 2023, 12(12):2388.
[19] TANG L, CHEN Y C, JIANG Z B, et al.Purification, partial characterization and bioactivity of sulfated polysaccharides from Grateloupia livida[J].International Journal of Biological Macromolecules, 2017, 94:642-652.
[20] 张叶飞. 产色素粘质沙雷氏菌的分离鉴定及含灵菌红素合成酶的载体构建[D].成都:成都理工大学, 2019.
ZHANG Y F.Isolation and identification of pigmented Serratia marcescens and construction of vector containing prodigiosin synthase[D].Chengdu:Chengdu University of Technology, 2019.
[21] 刘思航, 邹宜均, 常菲菲, 等.一株高产灵菌红素粘质沙雷氏菌的分离鉴定及发酵条件优化[J].应用与环境生物学报, 2018, 24(1):26-32.
LIU S H, ZOU Y J, CHANG F F, et al.Isolation and identification of Serratia marcescens producing high levels of prodigiosin and its fermentation optimization[J].Chinese Journal of Applied and Environmental Biology, 2018, 24(1):26-32.
[22] XU H, WANG S S, TIAN Y J, et al.2-Keto-D-gluconic acid and prodigiosin producing by a Serratia marcescens[J].Preparative Biochemistry & Biotechnology, 2021, 51(7):678-685.
[23] ARIVIZHIVENDHAN K V, MAHESH M, BOOPATHY R, et al.A novel method for the extraction of prodigiosin from bacterial fermenter integrated with sequential batch extraction reactor using magnetic iron oxide[J].Process Biochemistry, 2016, 51(10):1731-1737.
[24] 李雪. 粘质沙雷氏菌次级代谢产物灵菌红素的结构鉴定及生物学活性研究[D].济南:齐鲁工业大学, 2021.
LI X.Structure identification and biological activity of prodigiosin, a secondary metabolite of Serratia marcescens[D].Jinan:Qilu University of Technology, 2021.
[25] JARDAK M, ATOISSI A, MSALBI D, et al.Antibacterial, antibiofilm and cytotoxic properties of prodigiosin produced by a newly isolated Serratia sp.C6LB from a milk collection center[J].Microbial Pathogenesis, 2022, 164:105449.
[26] VIJAY D, ALSHAMSI N S, MOUSSA Z, et al.Extraction of the anticancer and antimicrobial agent, prodigiosin, from Vibrio gazogenes PB1 and its identification by 1D and 2D NMR[J].Molecules, 2022, 27(18):6030.
[27] ARIVUSELVAM R, DERA A A, PARWEEN ALI S, et al.Isolation, identification, and antibacterial properties of prodigiosin, a bioactive product produced by a new Serratia marcescens JSSCPM1 strain:Exploring the biosynthetic gene clusters of Serratia species for biological applications[J].Antibiotics, 2023, 12(9):1466.
[28] WILF N M, SALMOND G P C.The stationary phase sigma factor, RpoS, regulates the production of a carbapenem antibiotic, a bioactive prodigiosin and virulence in the enterobacterial pathogen Serratia sp.ATCC 39006[J].Microbiology, 2012, 158(Pt3):648-658.
[29] BHUPATHI P, ELHASSAN A-ELGADIR T M, MOHAMMED ALI R H, et al.Fluorescence resonance energy transfer (FRET)-based sensor for detection of foodborne pathogenic bacteria:A review[J].Critical Reviews in Analytical Chemistry, 2023: 1-18.
[30] TOUSHIK S H, PARK J H, KIM K, et al.Antibiofilm efficacy of Leuconostoc mesenteroides J.27-derived postbiotic and food-grade essential oils against Vibrio parahaemolyticus, Pseudomonas aeruginosa, and Escherichia coli alone and in combination, and their application as a green preservative in the seafood industry[J].Food Research International, 2022, 156:111163.
[31] CHOI S Y, LIM S, YOON K H, et al.Biotechnological activities and applications of bacterial pigments violacein and prodigiosin[J].Journal of Biological Engineering, 2021, 15(1):10.
[32] LIM S, BHAK J, JEON S, et al.The kiss of death:Serratia marcescens antibacterial activities against Staphylococcus aureus requires both de novo prodigiosin synthesis and direct contact[J].Microbiology Spectrum, 2022, 10(3):e0060722.
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