To investigate the inhibitory ability of strains X3-2B and 37X-3 on xanthine oxidase(XOD)and their alleviating effect on hyperuricemia (HUA) in mice, this study used HPLC to determine the content of xanthine and uric acid (UA) in the medium.The strains were cultured in a medium containing xanthine and XOD for different durations (20, 40, 60, 80 and 100 min).This study also adjusted the pH and bile salt concentration of the medium to study the inhibitory ability of XOD and the acid and alkali resistance of strains X3-2B and 37X-3 in vitro.In addition, sixty KM mice were divided into five groups, including control group (Group C), model group (Group M), X3-2B group (Group X), 37X-3 group (Group L), and allopurinol group (Group P).All groups, except for Group C, were fed high-purine chow.The mice were fed for 4 weeks, and the corresponding doses of strains X3-2B, 37X-3, and allopurinol were administered to groups X, L, and P respectively.This study investigated the mitigating effects of strains X3-2B and 37X-3 on HUA in mice by analyzing physiological and biochemical indexes, renal pathology sections, and intestinal tissues of the mice for XOD activity.Results showed that strains X3-2B and 37X-3 effectively inhibited XOD-catalyzed xanthine production of UA.At pH 2 and bile salt addition of 0.3%, the viable bacterial counts of X3-2B and 37X-3 were 6.4×107 CFU/g (X3-2B), 9.3×107 CFU/g (37X-3), and 2.1×106 CFU/g (X3-2B), 2.8×106 CFU/g (37X-3) respectively.After four weeks of feeding, there was a significant decrease in serum levels of UA, BUN, and Cr in mice in groups X and L (P<0.05).The renal units were structurally intact, and the activity of XOD in intestinal tissues was inhibited (P<0.05).The study concluded that strains X3-2B and 37X-3 were able to effectively inhibit XOD activity and reduce UA production.Furthermore, these strains were found to exert XOD inhibition function in the intestinal tract of HUA mice and alleviate HUA in mice.
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