食品与发酵工业 >

2024 , Vol. 50 >Issue 21: 206 - 215

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038131

研究报告

弯萼金丝桃总多酚提取工艺优化及抗氧化、降糖活性研究

  • 时文盼 ,
  • 郭嘉欣 ,
  • 蔺永刚 ,
  • 柴雨阳 ,
  • 郭玉锦 ,
  • 武斌 ,
  • 王俊龙
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  • 1(伊犁师范大学 新疆生物质资源清洁转化与高值化利用重点实验室,新疆 伊宁,835000)
    2(长春大学 食品科学与工程学院,吉林 长春,130000)
    3(重庆医科大学 儿科学院,重庆,404100)
第一作者:硕士,实验师(王俊龙实验师为通信作者,E-mail:974881461@qq.com)

收稿日期: 2023-12-01

  修回日期: 2023-12-21

  网络出版日期: 2024-11-28

基金资助

伊犁师范大学校级科研项目(2022YSYB030);伊犁州科技计划项目(YJC2023A15)

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Optimization of total phenolics extraction process from Hypericum curvisepalum and its antioxidant and hypoglycemic activities

  • SHI Wenpan ,
  • GUO Jiaxin ,
  • LIN Yonggang ,
  • CHAI Yuyang ,
  • GUO Yujin ,
  • WU Bin ,
  • WANG Junlong
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  • 1(Xinjiang Key Laboratory of Clean Conversion and High Value Utilization of Biomass Resources, Yili Normal University, Yining 835000, China)
    2(College of Food Science and Engineering, Changchun University, Changchun 130000, China)
    3(College of Pediatrics, Chongqing Medical University, Chongqing 404100, China)

Received date: 2023-12-01

  Revised date: 2023-12-21

  Online published: 2024-11-28

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摘要

弯萼金丝桃是一种灌木类植物,具有多种生物活性。该研究比较了热水浸提、冷浸醇提、酸解、酶解和超声提取5种方法,并采用响应面法优化了弯萼金丝桃总多酚(total polyphenol,TP)超声提取工艺,鉴定并研究了其主要苷元和抗氧化、降糖活性。结果表明,5种提取工艺中,超声提取效果最佳,优化后最佳提取条件为,提取温度(A)62 ℃、提取时间(B)48 min、乙醇体积分数(C)67%和液料比(D)64∶1(mL∶g),TP提取率为(19.78±0.03)%。通过UPLC鉴定出9种主要多酚类化合物,其中含量最高的为槲皮素(4.725 0 mg/g)。当TP质量浓度为0.046 2 mg/L时,对DPPH自由基、ABTS阳离子自由基和羟自由基的清除率分别为90.81%、73.91%、60.73%,并且具有一定还原能力,对α-葡萄糖苷酶的抑制率达到98.36%。试验结果证实,超声提取能有效提高弯萼金丝桃中TP的提取率,为弯萼金丝桃在医药等领域高值化产品的开发提供实验基础。

关键词： 弯萼金丝桃; 总多酚; 提取工艺; 苷元; 抗氧化活性; 降糖活性

本文引用格式

时文盼 , 郭嘉欣 , 蔺永刚 , 柴雨阳 , 郭玉锦 , 武斌 , 王俊龙 . 弯萼金丝桃总多酚提取工艺优化及抗氧化、降糖活性研究[J]. 食品与发酵工业, 2024 , 50(21) : 206 -215 . DOI: 10.13995/j.cnki.11-1802/ts.038131

Abstract

Hypericum curvisepalum is a shrub plant with a variety of biological activities.This study compared five methods:hot water extraction, cold alcohol extraction, acid hydrolysis, enzymatic hydrolysis, and ultrasonic extraction.Response surface methodology was used to optimize the ultrasonic extraction process of total polyphenols (TP) of Hypericum curvisepalum.Additionally, the study aimed to identify the main aglycones of TP and investigate their antioxidant and hypoglycemic activities.The results indicated that ultrasonic extraction yields the most favorable outcomes among the five extraction processes.After optimization, the optimal extraction conditions were as follows:extraction temperature (A) at 62 ℃, extraction time (B) of 48 minutes, ethanol volume fraction (C) at 67%, and liquid-to-solid ratio (D) at 64∶1 (mL∶g).The extraction rate of TP is measured to be (19.78±0.03)%.Nine major polyphenolic compounds were identified by UPLC, of which the highest content was quercetin (4.725 0 mg/g).When the TP mass concentration is 0.046 2 mg/L, the scavenging rates of DPPH, ABTS and ·OH free radicals were 90.81%, 73.91%, and 60.73% respectively, with a certain reducing ability.The inhibition rate of α-glucosidase reached 98.36%.The test results confirmed that ultrasonic extraction can effectively improve the extraction rate of total polyphenols (TP) in Hypericum curvisepalum, providing an experimental basis for the development of high-value products of Hypericum curvisepalum in medicine and other fields.

Key words： Hypericum curvisepalum; total polyphenols; extraction process; glycogen; antioxidant activity; hypoglycemic activity

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