LED深紫外灯作为替代传统汞灯的新兴力量,在未来食品杀菌方向有着广泛的应用前景。该研究首先以大肠杆菌为实验对象,获得合适的LED深紫外灯组合方案。其次通过所得到的LED深紫外灯组合分别对大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌进行不同时长的深紫外线杀菌处理,探究深紫外线照射时间对不同致病菌杀菌率的影响。最后以草莓为实验材料,通过定期调查其坏果率、腐败指数、失重率及感官变化,探究LED深紫外线照射对草莓保鲜的效果。研究发现染菌平板正上方与侧方各有3个LED深紫外灯时,杀菌效果最好。随着LED深紫外线处理时长的延长,杀菌率逐渐上升,且对LED深紫外线的敏感性:金黄色葡萄球菌<铜绿假单胞菌<大肠杆菌。草莓采摘后进行适当时长的LED深紫外线处理对控制病变、保障品质等具有良好的效果,LED深紫外线处理与密封冷藏对提高草莓保鲜效果具有协同作用。
As an alternative of traditional mercury lamps, LED deep ultraviolet lamps have a wide application prospect in the food sterilization field.Firstly, by taking took Escherichia coli as the experimental object, an appropriate combination scheme of deep ultraviolet lamp was explored.Secondly, using the combination of LED deep ultraviolet lamp to sterilize E.coli, Staphylococcus aureus and Pseudomonas aeruginosa with different duration of deep ultraviolet light, the effect of deep ultraviolet irradiation time on the bactericide rate of different pathogenic bacteria was studied.Finally, the effect of LED deep ultraviolet radiation on strawberry preservation was explored by regular investigation of their bad fruit rate, decay index, weight loss rate and sensory changes.In the study, it was found that the sterilizing effect was best when there were three LED deep ultraviolet lamps on the top and the side of the plate, and the bactericide rate increased with the extension of irradiation time.The sensitivity to LED deep ultraviolet light showed:S.aureus<P.aeruginosa<E.coli.After strawberries were picked, LED deep ultraviolet treatment for a proper duration had a good effect on disease control and quality assurance.In addition, LED deep ultraviolet treatment and sealed refrigeration had a synergistic effect on improving strawberry freshness preservation effect.
[1] STEELE M, ODUMERU J.Irrigation water as source of foodborne pathogens on fruit and vegetables[J].Journal of Food Protection, 2004, 67(12):2839-2849.
[2] WEI C J, ZHONG J L, HU T, et al.Simultaneous detection of Escherichia coli O157∶H7, Staphylococcus aureus and Salmonella by multiplex PCR in milk[J].3 Biotech, 2018, 8(1):76.
[3] JANA M, ADRIANA V, EVA K.Evaluation of DNA extraction methods for culture-independent real-time PCR-based detection of Listeria monocytogenes in cheese[J].Food Analytical Methods, 2020, 13(3):667-677.
[4] MICEK S T, LLOYD A E, RITCHIE D J, et al.Pseudomonas aeruginosa bloodstream infection:Importance of appropriate initial antimicrobial treatment[J].Antimicrobial Agents and Chemotherapy, 2005, 49(4):1306-1311.
[5] TACK D M, MARDER E P, GRIFFIN P M, et al.Preliminary incidence and trends of infections with pathogens transmitted commonly through food-foodborne diseases active surveillance network, 10 U.S.sites, 2015—2018[J].MMWR.Morbidity and Mortality Weekly Report, 2019, 68(16):369-373.
[6] 沈生文.食品杀菌技术概述[J].食品安全导刊, 2020(35):52.
SHEN S W.Overview of food sterilization technology[J].China Food Safety Magazine, 2020(35):52.
[7] 郑云芳, 王晓雯, 钟丽琪.冷杀菌技术在食品中的应用[J].现代食品, 2017,23(13):67-68.
ZHENG Y F, WANG X W, ZHONG L Q.Application of cold sterilization technology in food[J].Modern Food, 2017,23(13):67-68.
[8] SHIN J Y, KIM S J, KIM D K, et al.Fundamental characteristics of deep-UV light-emitting diodes and their application to control foodborne pathogens[J].Applied and Environmental Microbiology, 2015, 82(1):2-10.
[9] 部建雯. 短波紫外线照射对樱桃番茄果实成熟的影响及机理研究[D].杭州:浙江大学, 2014.
BU J W.Study on the effects and mechanism of UV-C irradiation on ripening in postharvest cherry tomato fruit[D].Hangzhou:Zhejiang University, 2014.
[10] WON W S, TRAN L G, PARK W T, et al.UV-LEDs for the disinfection and bio-sensing applications[J].International Journal of Precision Engineering and Manufacturing, 2018, 19(12):1901-1915.
[11] 李乐. 基于深紫外LED的杀菌系统在汽车中的应用研究[D].武汉:华中科技大学, 2017.
LI L.Study on application of DUV-LED sterilization system in automobile[D].Wuhan:Huazhong University of Science and Technology, 2017.
[12] 赵磊, 王丹, 马越, 等.短波紫外线照射对鲜切黄瓜片品质的影响[J].食品工业, 2019, 40(5):205-209.
ZHAO L, WANG D, MA Y, et al.Effect of ultraviolet C irradiation on the quality of fresh cut cucumber slices[J].The Food Industry, 2019, 40(5):205-209.
[13] 于刚, 栾雨时, 安利佳.UV-C处理对蓝莓贮藏保鲜及品质的影响[J].食品研究与开发, 2013, 34(2):92-95.
YU G, LUAN Y S, AN L J.Effects of UV-C treatment on fresh-keeping and storage quality of blueberry[J].Food Research and Development, 2013, 34(2):92-95.
[14] 田竹希, 龙明秀, 李咏富, 等.短波紫外线照射和60Co-γ辐照处理对大樱桃贮藏品质的影响[J].食品科学, 2019, 40(23):269-276.
TIAN Z X, LONG M X, LI Y F, et al.Effects of short-wave ultraviolet(UV-C) light and 60Co-γ irradiation on the storage quality of cherry fruits[J].Food Science, 2019, 40(23):269-276.
[15] BARNEWALL R E, BISCHOFF W E.Removal of SARS-CoV-2 bioaerosols using ultraviolet air filtration[J].Infection Control and Hospital Epidemiology, 2021, 42(8):1014-1015.
[16] LEE Y W, YOON H D, PARK J H, et al.Application of 265-nm UVC LED lighting to sterilization of typical gram negative and positive bacteria[J].Journal of the Korean Physical Society, 2018, 72(10):1174-1178.
[17] GAYÁN E, ÁLVAREZ I, CONDÓN S.Inactivation of bacterial spores by UV-C light[J].Innovative Food Science & Emerging Technologies, 2013, 19:140-145.
[18] 林萍, 潘珍瑜, 江建真, 等.LED紫外线杀菌效果实验研究[J].科学与信息化, 2018, (27):192;194.
LIN P, PAN Z Y, JIANG J Z, et al.Experimental study on LED ultraviolet sterilization effect[J].Science and Informatization, 2018, (27):192;194.
[19] 方方, 林凯旋, 吕善斌, 等.UV LED紫外线杀菌效果的评估[J].照明工程学报, 2020, 31(1):9-10.
FANG F, LIN K X, LYU S B, et al.Evaluation of ultraviolet sterilization effect of UV LED[J].China Illuminating Engineering Journal, 2020, 31(1):9-10.
[20] 郭小磊. 草莓采后腐败菌的致病规律及抑菌研究[D].石家庄:河北科技大学, 2016.
GUO X L.Study on the pathogenic mechanism & control of spoilage fungi on postharvest strawberry[D].Shijiazhuang:Hebei University of Science and Technology, 2016.
[21] 唐甜甜, 解新方, 任雪, 等.草莓贮藏保鲜技术的研究进展[J].食品工业科技, 2020, 41(5):332-339.
TANG T T, XIE X F, REN X, et al.Research progress on storage and preservation technology of strawberry[J].Science and Technology of Food Industry, 2020, 41(5):332-339.
[22] 孔繁渊. 热空气和MeJA复合处理对草莓果实采后病害影响及其机理研究[D].南京:南京农业大学, 2010.
KONG F Y.Study on effect and mechanism of hot air treatment combined with MeJA on postharvest disease of strawberry fruit[D].Nanjing:Nanjing Agricultural University, 2010.
[23] 傅述敏. 食品微生物学[M].北京:中国商业出版社, 1990:183-186.
FU S M.Food Microbiology[M].Beijing:China Business Press, 1990:183-186.
[24] ERKAN M, WANG S Y, WANG C Y.Effect of UV treatment on antioxidant capacity, antioxidant enzyme activity and decay in strawberry fruit[J].Postharvest Biology and Technology, 2008, 48(2):163-171.
[25] 陈铭中. 短波紫外线照射对采后香蕉贮藏特性的影响及其胁迫响应机制[D].湛江:广东海洋大学, 2022.
CHEN M Z.Effects of short-wave ultraviolet radiation on postharvest storage characteristics of banana and its stress response mechanism[D].Zhanjiang:Guangdong Ocean University, 2022.
