将大豆分离蛋白(soy protein isolate,SPI)和大米粉(rice flour,RF)按比例混合后水浴加热制备复合凝胶,以SPI 100%为对照,考查RF的添加量对SPI与RF形成的复合凝胶特性的影响及复合凝胶形成机制。结果表明,添加RF制备的SPI-RF复合凝胶的持水性和黏度系数均高于对照样品(P＜0.05);随着RF添加量的增加,SPI-RF复合凝胶的硬度、弹性、胶黏性、咀嚼性皆呈先升后降的趋势(P＜0.05);RF添加量为40%时,复合凝胶的持水性、硬度、弹性、胶黏性和咀嚼性均达最大值,依次为87.33%、1 739.491 g、0.230、1 104.656和1 084.008。分子对接结果显示,SPI中蛋白质与RF中的淀粉分子主要通过氢键和疏水作用形成复合凝胶,用原子力显微镜(atomic force microscope,AFM)观察微观结构,可见来自SPI和RF中蛋白质、淀粉分子的单条链、多条链及聚集体的存在。RF的添加能显著提高SPI的凝胶特性,在植物基产品加工中有应用潜力。
Soy protein isolate (SPI) and rice flour (RF) were mixed in an appropriate proportion and then heated in the water bath to prepare SPI-RF composite gel.SPI 100% was used as the control to investigate the effects of different concentrations of RF on the gel properties, the gelatinization mechanisms of SPI-RF composite gel was revealed.Results showed that the water holding capacity and viscosity coefficient of the composite gel were higher than those of the control sample (P<0.05).The hardness, elasticity, viscosity, and chewiness of the gel raised initially and then reduced (P<0.05) as the amount of RF increased.The water holding capacity, hardness, elasticity, adhesiveness, and chewiness of SPI-RF 40% gel reached the maximum values, which were 87.33%, 1 739.491 g, 0.230, 1 104.656, and 1 084.008, respectively.Molecular docking indicated that the main interaction force of protein-starch in the gel were the hydrogen bond and hydrophobic interaction.The microstructure was examined by atomic force microscopy (AFM), the single chain, multiple chains, and chain aggregates were showed.Obviously, the addition of RF improved significantly the gel properties of SPI, that would be potential for application in plant-based products.
[1] SCHREUDERS F K G, DEKKERS B L, BODNÁR I, et al.Comparing structuring potential of pea and soy protein with gluten for meat analogue preparation[J].Journal of Food Engineering, 2019, 261:32-39.
[2] KERSLAKE E, KEMPER J A, CONROY D.What′s your beef with meat substitutes? Exploring barriers and facilitators for meat substitutes in omnivores, vegetarians, and vegans[J].Appetite, 2022, 170:105864.
[3] ESTELL M, HUGHES J, GRAFENAUER S.Plant protein and plant-based meat alternatives:Consumer and nutrition professional attitudes and perceptions[J].Sustainability, 2021, 13(3):1478.
[4] CHEN Q L, ZHANG J C, ZHANG Y J, et al.Rheological properties of pea protein isolate-amylose/amylopectin mixtures and the application in the high-moisture extruded meat substitutes[J].Food Hydrocolloids, 2021, 117:106732.
[5] BI C H, LI D, WANG L J, et al.Effect of LBG on the gel properties of acid-induced SPI gels[J].LWT, 2017, 75:1-8.
[6] JUNG A H, HWANG J H, JUN S, et al.Application of ohmic cooking to produce a soy protein-based meat analogue[J].LWT, 2022, 160:113271.
[7] 周凤超,林国荣,汪秀妹,等.改性马铃薯淀粉对肌原纤维蛋白凝胶特性的影响[J].食品科学,2020,41(4):86-95.
[8] CHOI H W, RYOO C, HAHN J, et al.Development of a novel technology for high-moisture textured soy protein using a vacuum packaging and pressurized heat (vacuum-autoclaving) treatment[J].Food Chemistry, 2023, 399: 133887.
[9] HU T, ZHANG Z, HU H, et al.A comprehensive study on self-assembly and gelation of C13-dipeptides-from design strategies to functionalities[J].Biomacromolecules, 2020, 21(2):670-679.
[10] PALANISAMY M, TÖPFL S, AGANOVIC K, et al.Influence of iota carrageenan addition on the properties of Soya protein meat analogues[J].LWT, 2018, 87:546-552.
[11] YANG C H, ZHONG F, DOUGLAS GOFF H, et al.Study on starch-protein interactions and their effects on physicochemical and digestible properties of the blends[J].Food Chemistry, 2019, 280:51-58.
[12] FOEGEDING E A.Functional properties of turkey salt-soluble proteins[J].Journal of Food Science, 1987, 52(6):1495-1499.
[13] NADA H, LEE K, GOTINA L, et al.Identification of novel discoidin domain receptor 1 (DDR1) inhibitors using E-pharmacophore modeling, structure-based virtual screening, molecular dynamics simulation and MM-GBSA approaches[J].Computers in Biology and Medicine, 2022, 142:105217.
[14] TAO H, LU F, ZHU X F, et al.Removing surface proteins promote the retrogradation of wheat starch[J].Food Hydrocolloids, 2021, 113:106437.
[15] 潘泓杉, 马高兴, 裴斐, 等.金针菇多糖对大豆分离蛋白凝胶的增强作用及其结构表征[J].食品科学, 2022, 43(20):102-108.
PAN H S, MA G X, PEI F, et al.Enhancing effect of Flammulina velutipes polysaccharide on gelation properties of soybean protein isolate and structural characterization of mixed gels[J].Food Science, 2022, 43(20):102-108.
[16] ZOU Y, YU X Y, ZHENG Q W, et al.Effect of beating process on the physicochemical and textural properties of meat analogs prepared with Cordyceps militaris fruiting body[J].International Journal of Food Engineering, 2022, 18(2):153-160.
[17] XING J J, LI D, WANG L J, et al.Relationship between biphasic endotherms and multi-stage gelatinization of corn starch in excess water[J].LWT-Food Science and Technology, 2017, 81:335-342.
[18] ZHAO H S, MA Z, JING P.Interaction of soy protein isolate fibrils with betalain from red beetroots:Morphology, spectroscopic characteristics and thermal stability[J].Food Research International, 2020, 135:109289.
[19] 费贵强, 谢萍浩, 王海花, 等.磺酸盐型高分子表面活性剂的制备及性能研究[J].应用化工, 2020, 49(1):114-119.
FEI G Q, XIE P H, WANG H H, et al.Preparation and properties of sulfonate high polymer surfactants[J].Applied Chemical Industry, 2020, 49(1):114-119.
[20] RUSSELL P L.Gelatinisation of starches of different amylose/amylopectin content.A study by differential scanning calorimetry[J].Journal of Cereal Science, 1987, 6(2):133-145.
[21] VIVIAN J T, CALLIS P R.Mechanisms of tryptophan fluorescence shifts in proteins[J].Biophysical Journal, 2001, 80(5):2093-2109.
[22] DING F, BORREGUERO J M, BULDYREY S V, et al.Mechanism for the α-helix to β-hairpin transition[J].Proteins:Structure, Function, and Bioinformatics, 2003, 53(2):220-228.
[23] CUI Z M, KONG X Z, CHEN Y M, et al.Effects of rutin incorporation on the physical and oxidative stability of soy protein-stabilized emulsions[J].Food Hydrocolloids, 2014, 41:1-9.
[24] 赵钜阳, 袁惠萍, 姚恒喆, 等.pH值对儿茶素-大豆分离蛋白复合物结构与乳化性的影响[J].食品科学技术学报, 2023, 41(3):127-138.
ZHAO J Y, YUAN H P, YAO H Z, et al.Effects of pH on structure and emulsibility of catechin-soy protein isolate complex[J].Journal of Food Science and Technology, 2023, 41(3):127-138.
[25] 关曼. 壳聚糖/大豆分离蛋白复合膜的制备、性能及应用[D].上海:上海海洋大学, 2016.
GUAN M.Preparation, properties and application of chitosan/soy protein isolate composite membrane[D].Shanghai:Shanghai Ocean University, 2016.
[26] 陈兴. 槲皮素-食品乳液体系的构建及其生物可利用性[D].南昌:南昌大学, 2020.
CHEN X.Fabrication and bioavailability of quercetin-food emulsion systems[D].Nanchang:Nanchang University, 2020.
[27] YE J P, DENG L P, WANG Y R, et al.Impact of rutin on the foaming properties of soybean protein:Formation and characterization of flavonoid-protein complexes[J].Food Chemistry, 2021, 362:130238.
[28] 张红运, 杨立娜, 朱丹实, 等.基于原子力显微图像和流变学特性的大豆种皮多糖构象分析[J].中国食品学报, 2020, 20(9):38-46.
ZHANG H Y, YANG L N, ZHU D S, et al.Conformational analysis of polysaccharide from soybean hull based on atomic force microscope and rheological properties[J].Journal of Chinese Institute of Food Science and Technology, 2020, 20(9):38-46.
