为提升儿茶素的稳定性,扩大其在食品加工领域的应用范围,采用蔗糖共结晶法包埋不同质量分数(0%、0.4%、0.8%)的儿茶素,并将儿茶素共结晶微胶囊与鸡肉糜混合制备凝胶,探究其对热诱导鸡肉糜凝胶品质的影响。结果表明,蔗糖共结晶法可极大提升儿茶素的水溶性和消化稳定性。扫描电子显微镜和差示扫描量热仪显示儿茶素簇状附聚在重结晶蔗糖的多孔空间内,并改变了蔗糖的熔化特性。与空白对照组和游离儿茶素添加组相比,儿茶素共结晶微胶囊的添加可显著降低鸡肉糜凝胶的蒸煮损失率,并显著提高凝胶硬度和咀嚼性,改善凝胶持水力和白度值。此外,添加共结晶儿茶素的鸡肉糜凝胶滴水通道消失,孔隙较对照组明显减小。添加含0.8%质量分数儿茶素的共结晶微胶囊可使鸡肉糜凝胶的羰基含量降至最低,游离巯基和总巯基含量达最高值。综上所述,蔗糖共结晶法能明显提升儿茶素的环境稳定性,且共结晶儿茶素通过改善凝胶组织结构和降低蛋白质氧化程度,可有效增强热诱导鸡肉糜凝胶的质构和保水性。
The objective of this study was to improve the stability of catechins and expand their applications in food processing.To achieve this, catechins were encapsulated at different mass fractions (0%, 0.4%, 0.8%) in co-crystallized sucrose.Meanwhile, catechin co-crystallized microcapsules were mixed with chicken batter to prepare gels and investigate their effects on heat-induced gelling properties.The study found that the co-crystallization treatment significantly improved the water solubility and digestive stability of catechin.Scanning electron microscope (SEM) and differential scanning calorimeter (DSC) analyses confirmed that catechin clusters adhered to the porous space of recrystallized sucrose and modified the melting properties of sucrose.Compared to the blank control and free catechin-added groups, the chicken batters added with encapsulated catechin significantly reduced cooking loss and increased gel hardness, chewiness, water-holding capacity, and whiteness values.The addition of encapsulated catechin reduced the drip channels and aggregated voids in the chicken batter compared to the control group.Furthermore, the incorporation of co-crystallized microcapsules containing 0.8% of catechin minimized the carbonyl content and maximized the free and total sulfhydryl content of chicken mince gels.In conclusion, the sucrose co-crystallization method can significantly enhance the environmental stability of catechins, and the co-crystallized catechins can effectively improve the texture and water retention of heat-induced chicken batters by improving the gel organization and reducing the degree of protein oxidation.
[1] 孙京新, 雷甜甜, 丁翠云, 等.不同配方对鸡肉丸品质的影响[J].肉类研究, 2009,23(3):30-33.
SUN J X, LEI T T, DING C Y, et al.Influence of different ingredients on the quality of chicken meatball[J].Meat Research, 2009,23(3):30-33.
[2] 李宁宁, 李钊, 赵圣明, 等.地皮菜添加量对鸡胸肉糜凝胶特性的影响[J].食品科学, 2021, 42(2):53-59.
LI N N, LI Z, ZHAO S M, et al.Effect of Nostoc commune on gelation and rheological properties of chicken breast meat batters[J].Food Science, 2021, 42(2):53-59.
[3] ZHU D Y, KANG Z L, MA H J, et al.Effect of sodium chloride or sodium bicarbonate in the chicken batters:A physico-chemical and Raman spectroscopy study[J].Food Hydrocolloids, 2018, 83:222-228.
[4] BANERJEE R, VERMA A, SIDDIQUI M, et al. Natural Antioxidants[M]. America: CRC Press, 2016:95-140.
[5] GADKARI P V, BALARAMAN M.Catechins:Sources, extraction and encapsulation:A review[J].Food and Bioproducts Processing, 2015,93:122-138.
[6] WANG D L, WANG J J, SUN J C, et al.Degradation kinetics and isomerization of 5-O-caffeoylquinic acid under ultrasound:Influence of epigallocatechin gallate and vitamin C[J].Food Chemistry:X, 2021,12:100147.
[7] RAI K, CHHANWAL N, SHAH N N, et al.Encapsulation of ginger oleoresin in co-crystallized sucrose:Development, characterization and storage stability[J].Food & Function, 2021, 12(17):7964-7974.
[8] YANG L, SHEN S Y, WANG Z N, et al.New value-added sugar and brown sugar products from sugarcane:A commercial approach[J].Sugar Tech, 2020, 22(5): 853-857.
[9] CHEZANOGLOU E, GOULA A M.Co-crystallization in sucrose:A promising method for encapsulation of food bioactive components[J].Trends in Food Science & Technology, 2021, 114:262-274.
[10] LÓPEZ-CÓRDOBA A, DELADINO L, AGUDELO-MESA L, et al.Yerba mate antioxidant powders obtained by co-crystallization:Stability during storage[J].Journal of Food Engineering, 2014, 124:158-165.
[11] WEN S Y, ZHOU G H, LI L, et al.Effect of cooking on in vitro digestion of pork proteins:A peptidomic perspective[J].Journal of Agricultural and Food Chemistry, 2015, 63(1):250-261.
[12] TURGUT S S, SOYER A, IŞıKÇı F.Effect of pomegranate peel extract on lipid and protein oxidation in beef meatballs during refrigerated storage[J].Meat Science, 2016, 116:126-132.
[13] LÓPEZ-CÓRDOBA A, GALLO L, BUCALÁ V, et al.Co-crystallization of zinc sulfate with sucrose:A promissory strategy to render zinc solid dosage forms more palatable[J].Journal of Food Engineering, 2016, 170:100-107.
[14] BEHNAMNIK A, VAZIFEDOOST M, DIDAR Z, et al.Evaluation of physicochemical, structural, and antioxidant properties of microencapsulated seed extract from Securigera securidaca by co-crystallization method during storage time[J].Biocatalysis and Agricultural Biotechnology, 2021, 35:102090.
[15] DELADINO L, NAVARRO A S, MARTINO M N.Microstructure of minerals and yerba mate extract co-crystallized with sucrose[J].Journal of Food Engineering, 2010, 96(3):410-415.
[16] BHANDARI B R, HARTEL R W.Co-crystallization of sucrose at high concentration in the presence of glucose and fructose[J].Journal of Food Science, 2002, 67(5):1797-1802.
[17] TZATSI P, GOULA A M.Encapsulation of extract from unused chokeberries by spray drying, co-crystallization, and ionic gelation[J].Waste and Biomass Valorization, 2021, 12(8):4567-4585.
[18] SHARMA G, SHARMA S, KUMAR A, et al.Guar gum and its composites as potential materials for diverse applications:A review[J].Carbohydrate Polymers, 2018, 199:534-545.
[19] ZHANG M C, XIA X F, LIU Q, et al.Changes in microstructure, quality and water distribution of porcine longissimus muscles subjected to ultrasound-assisted immersion freezing during frozen storage[J].Meat Science, 2019, 151:24-32.
[20] 赵泽润, 邢通, 赵雪, 等.大豆分离蛋白添加量对低盐木质化鸡肉糜凝胶特性的影响[J].食品科学, 2022, 43(6):49-56.
ZHAO Z R, XING T, ZHAO X, et al.Effect of addition of soy protein isolate on the gelation properties of low-salt wooden chicken breast meat batter[J].Food Science, 2022, 43(6):49-56.
[21] CUNHA L C M, MONTEIRO M L G, COSTA-LIMA B R C, et al.Effect of microencapsulated extract of pitaya (Hylocereus costaricensis) peel on color, texture and oxidative stability of refrigerated ground pork patties submitted to high pressure processing[J].Innovative Food Science & Emerging Technologies, 2018, 49:136-145.
[22] 杨玉玲, 周磊, 游远, 等.氧化对肌原纤维蛋白热诱导凝胶质构特性及保水性的影响[J].中国农业科学, 2018, 51(18):3570-3581.
YANG Y L, ZHOU L, YOU Y, et al.The effects of oxidation on textural properties and water holding capacity of heat-induced myofibrillar protein gel[J].Scientia Agricultura Sinica, 2018, 51(18):3570-3581.
[23] CAVALLIERI A L F, DA CUNHA R L.The effects of acidification rate, pH and ageing time on the acidic cold set gelation of whey proteins[J].Food Hydrocolloids, 2008, 22(3):439-448.
[24] JONGBERG S, GISLASON N E, LUND M N, et al.Thiol-quinone adduct formation in myofibrillar proteins detected by LC-MS[J].Journal of Agricultural and Food Chemistry, 2011, 59(13):6900-6905.