食品与发酵工业 >

2024 , Vol. 50 >Issue 22: 334 - 338

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.039088

分析与检测

基于稳定氢同位素特征的天然番茄红素鉴别研究

  • 罗静仪 ,
  • 武竹英 ,
  • 冯迪 ,
  • 王道兵 ,
  • 符艳美 ,
  • 安红梅 ,
  • 杨清山 ,
  • 刘洋 ,
  • 岳红卫 ,
  • 钟其顶 ,
  • 谢周杰
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  • 1(天津科技大学 生物工程学院,天津,300457)
    2(国家市场监管技术创新中心(轻工消费品质量安全),北京,100015)
    3(中轻技术创新中心有限公司,北京,100015)
    4(河北晨光检测技术服务有限公司,河北 邯郸,300138)
第一作者:硕士研究生(钟其顶正高级工程师与王道兵正高级工程师为共同通信作者,E-mail:zhongqingding@163.com;wangdaobing111@163.com)

收稿日期: 2024-03-05

  修回日期: 2024-05-15

  网络出版日期: 2024-12-17

基金资助

中国轻工集科技创新基金项目(ZQ2022JC-JY03)

收起

Identification of natural lycopene based on hydrogen isotope characterization

  • LUO Jingyi ,
  • WU Zhuying ,
  • FENG Di ,
  • WANG Daobing ,
  • FU Yanmei ,
  • AN Hongmei ,
  • YANG Qingshan ,
  • LIU Yang ,
  • YUE Hongwei ,
  • ZHONG Qiding ,
  • XIE Zhoujie
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  • 1(College of Biological Engineering,Tianjin University of Science and Technology, Tianjin 300457, China)
    2(Technology Innovation Center of Light Industrial Consumer Goods Quality and Safety for State Market Regulation, Beijing 100015, China)
    3(Sinolight Technology Innovation Centre Co.Ltd., Beijing 100015, China)
    4(Hebei Chenguang Detection Technology Service Co.Ltd., Handan 300138, China)

Received date: 2024-03-05

  Revised date: 2024-05-15

  Online published: 2024-12-17

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摘要

为了研究稳定氢同位素在番茄红素掺假检测领域的应用可行性,该研究利用高温裂解元素分析-稳定同位素比值质谱联用技术建立了测定不同来源番茄红素稳定氢同位素的方法。经验证,前处理后番茄红素样品纯度≥94.14%,前处理过程不会改变番茄红素氢稳定同位素比值的测定;该方法δ2H值重复性标准偏差为1.99‰,再现性标准偏差小于3‰。对13种番茄红素原料及番茄红素标准品的稳定氢同位素比值进行测定,人工合成的番茄红素δ2H值为-29.21‰~-56.22‰,天然来源的番茄红素δ2H值为-225.76‰~-256.98‰,发酵来源的番茄红素δ2H值为-81.35‰~-141.24‰。三者稳定氢同位素比值具有明显差异,该方法可以用于不同来源的番茄红素的鉴别。

关键词： 高温裂解元素分析-稳定同位素比值质谱; 番茄红素; δ2H; 真实性; 番茄红素原料

本文引用格式

罗静仪 , 武竹英 , 冯迪 , 王道兵 , 符艳美 , 安红梅 , 杨清山 , 刘洋 , 岳红卫 , 钟其顶 , 谢周杰 . 基于稳定氢同位素特征的天然番茄红素鉴别研究[J]. 食品与发酵工业, 2024 , 50(22) : 334 -338 . DOI: 10.13995/j.cnki.11-1802/ts.039088

Abstract

This study aimed to investigate the feasibility of the application of stable hydrogen isotopes in the field of lycopene adulteration detection.The method using the technique of temperature conversion/elementary analyzer-stable isotope ratio mass spectrometers (TC/EA-IRMS) was developed for the determination of stable hydrogen isotopes of lycopene from different sources.It was verified that the purity of lycopene samples was ≥94.14% after pretreatment, and the pretreatment process would not change the determination of lycopene hydrogen stable isotope ratio.The standard deviation of the δ2H value of the method was 1.99‰ for repeatability and the standard deviation of the reproducibility was less than 3‰.The stable hydrogen isotope ratios of 13 lycopene raw materials and lycopene standards were determined, the δ2H values of the synthetic lycopene ranged from -29.21‰--56.22‰, the δ2H values of the lycopene from natural sources ranged from -225.76 ‰--256.98 ‰, and the δ2H values of the lycopene from fermentation sources ranged from -81.35 ‰--141.24 ‰.The stabilized hydrogen isotope ratios of the three are significantly different, and the method can be used for the identification of lycopene from different sources.

Key words： temperature conversion/elementary analyzer-stable isotope ratio mass spectrometry (TC/EA-IRMS); lycopene; δ2H; authenticity; lycopene raw material

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