食品与发酵工业 >

2024 , Vol. 50 >Issue 24: 9 - 17

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038592

研究报告

乳酸萃取支撑液膜的构建及其在乳酸菌液态培养中的应用

  • 宋晓宁 ,
  • 姜静雅 ,
  • 曾剑华 ,
  • 王松 ,
  • 张兰威 ,
  • 公丕民
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  • 1(中国海洋大学 食品科学与工程学院,山东 青岛,266000)
    2(内蒙古国家乳业技术创新中心,内蒙古 呼和浩特市,150100)
    3(青岛尚德生物技术有限公司,山东 青岛,266000)
第一作者:硕士研究生(公丕民讲师为通信作者,E-mail:gongpimin@ouc.edu.cn)

收稿日期: 2024-01-15

  修回日期: 2024-02-06

  网络出版日期: 2024-12-30

基金资助

国家自然科学基金项目(32001664,32372289);中国博士后科学基金项目(2021T140632);山东省泰山产业领军人才项目(LJNY202101)

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Construction of lactic acid extraction supporting liquid membrane with application to liquid cultivation of lactic acid bacteria

  • SONG Xiaoning ,
  • JIANG Jingya ,
  • ZENG Jianhua ,
  • WANG Song ,
  • ZHANG Lanwei ,
  • GONG Pimin
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  • 1(College of Food Science and Engineering, Ocean University of China, Qingdao 266000, China)
    2(Inner Mongolia National Center of Technology Innovation for Dairy, Hohhot 150100, China)
    3(Qingdao Shangde Biotech Co.Ltd., Qingdao 266000, China)

Received date: 2024-01-15

  Revised date: 2024-02-06

  Online published: 2024-12-30

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摘要

利用碱中和解除乳酸胁迫是实现乳酸菌高密度培养的重要方法,但过量的碱也会因渗透压的增加造成菌体死亡。因此,在无渗透胁迫的情况下,原位分离乳酸是解决乳酸抑制的根本方法。该研究评估了利用支撑液膜原位分离乳酸来实现保加利亚乳杆菌sp1.1高密度培养的可行性。首先,利用聚偏二氟乙烯(polyvinylidene difluoride, PVDF)膜作为固体支撑膜,由食品源成分组成的天然低共熔溶剂(natural deep eutectic solvents, NADES)作为载体,NaOH作为接收相,构建了支撑液膜原位萃取系统。结果表明,30%(体积分数)油酸/薄荷醇(体积比2∶1)支撑液膜对保加利亚乳杆菌sp1.1促生长效果最佳,活菌数是未萃取的4.62倍。油酸的羧基与乳酸的羟基或羧基之间的强氢键实现了乳酸的萃取,而薄荷醇则负责稳定NADES。与正常培养相比,支撑液膜萃取乳酸将pH值稳定在4.3以上(完全抑制pH值为4.2),使保加利亚乳杆菌sp 1.1得以持续生长,然而,膜稳定性问题阻碍了保加利亚乳杆菌sp1.1的进一步增殖。NADES萃取系统有望用于乳酸菌的高密度培养。

关键词： 乳酸菌; 高密度培养; 乳酸萃取; 乳酸抑制; 支撑液膜

本文引用格式

宋晓宁 , 姜静雅 , 曾剑华 , 王松 , 张兰威 , 公丕民 . 乳酸萃取支撑液膜的构建及其在乳酸菌液态培养中的应用[J]. 食品与发酵工业, 2024 , 50(24) : 9 -17 . DOI: 10.13995/j.cnki.11-1802/ts.038592

Abstract

Utilisation of alkali to eliminate lactic acid (LA) stress during lactic acid bacteria (LAB) culturing is an important way to achieve a high-density culture of LAB, but excess alkali also causes cell death due to osmotic pressure.Hence, in situ isolation of LA during cultivation is a promising method without salt stress.In this study, the feasibility of using supported liquid membrane in situ extraction of LA to achieve a high-density culture of Lactobacillus delbrueckii ssp.bulgaricus sp1.1 was evaluated.Firstly, a supported liquid membrane extraction culture system was constructed using a polyvinylidene difluoride (PVDF) membrane as a solid support membrane, natural deep eutectic solvents (NADES) composed of food source components as a carrier, and NaOH as the receiving phase.Results showed that the 30% oleic acid/menthol (2∶1) supported liquid membrane had the best growth promotion effect of L.bulgaricus sp1.1, and produced 4.62-fold live bacteria numbers than that without extraction.Extraction of LA was achieved by the strong hydrogen bonds between the carboxyl groups of oleic acid and the hydroxyl or carboxyl groups of LA, while menthol was responsible for the stabilisation of NADES.SLM extraction cultures allow continuous growth of L.bulgaricus sp1.1 by maintaining a stable pH above 4.3 (fully inhibited pH of 4.2) compared to normal cultures.However, membrane stability issues prevented the long-term growth of L.bulgaricus sp1.1.The NADES solvent extraction system has the potential to be used in the development of high-density cultures of LAB.

Key words： lactic acid bacteria; high-density culture; lactic acid extraction; lactic acid inhibition; supported liquid membrane

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