食品与发酵工业 >

2024 , Vol. 50 >Issue 24: 169 - 175

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038426

研究报告

基于全基因组测序的菌株发酵粘液乳杆菌(Limosilactobacillus fermentum)FUA033的安全性和益生特性分析

  • 母淑婷 ,
  • 华梓延 ,
  • 赵双双 ,
  • 房耀维 ,
  • 杨光 ,
  • 侯晓月 ,
  • 宫艳艳 ,
  • 桂家进 ,
  • 刘姝
展开
  • 1(江苏省海洋生物产业技术协同创新中心,江苏 连云港,222005)
    2(江苏海洋大学 海洋食品与生物工程学院,江苏 连云港,222005)
    3(连云港市食品药品检验检测中心,江苏 连云港,222005)
第一作者:硕士研究生(刘姝教授为通信作者,E-mail:jdliushu@163.com)

收稿日期: 2024-01-03

  修回日期: 2024-02-07

  网络出版日期: 2024-12-30

基金资助

江苏省第五期“333工程”科研项目(BRA2019243);江苏省高等学校自然科学研究重大项目(20KJA550001);江苏省研究生科研与实践创新计划项目(KYCX2023_32);大学生创新创业训练计划立项项目(SY202311641638001)

收起

Safety and probiotic characteristics of Limosilactobacillus fermentum FUA033 based on complete genome analysis

  • MU Shuting ,
  • HUA Ziyan ,
  • ZHAO Shuangshuang ,
  • FANG Yaowei ,
  • YANG Guang ,
  • HOU Xiaoyue ,
  • GONG Yanyan ,
  • GUI Jiajin ,
  • LIU Shu
Expand
  • 1(Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Lianyungang 222005, China)
    2(School of Marine Food and Bioengineering Jiangsu Ocean University, Lianyungang 222005, China)
    3(Lianyungang Food and Drug Inspection and Testing Center, Lianyungang 222005, China)

Received date: 2024-01-03

  Revised date: 2024-02-07

  Online published: 2024-12-30

Fold

摘要

发酵粘液乳杆菌(Limosilactobacillus fermentum) FUA033可以体外转化鞣花酸生成尿石素A,具有开发为新一代益生菌的潜力。为全面了解L.fermentum FUA033的遗传信息,该研究通过PacBio和Illumina相结合对该菌株进行全基因组测序,并从基因水平对菌株潜在的安全性和益生特性进行分析。结果表明,L.fermentum FUA033基因组全长2 150 545 bp,GC含量51.34%,不含质粒,编码基因2 215个;VFDB数据库与ARDB数据库注释结果显示L.fermentum FUA033中含有81个毒力基因和9个耐药基因,因菌株中不具有质粒和转座子,初步断定毒力因子与耐药基因不会转移;L.fermentum FUA033基因组中有耐酸基因(arcA、argH、napA等)19个,耐胆盐基因(ldhA、ykpA、pstB1等)16个,抗氧化基因(msrA、Trxs、ahpC等)11个,细胞黏附性基因(scpA、scpB、luxS)3个。研究结果初步揭示了L.fermentum FUA033潜在的安全性和益生特性,为菌株进一步的开发利用奠定了理论基础。

关键词： 发酵粘液乳杆菌; 尿石素A; 基因组测序; 安全性; 益生特性

本文引用格式

母淑婷 , 华梓延 , 赵双双 , 房耀维 , 杨光 , 侯晓月 , 宫艳艳 , 桂家进 , 刘姝 . 基于全基因组测序的菌株发酵粘液乳杆菌(Limosilactobacillus fermentum)FUA033的安全性和益生特性分析[J]. 食品与发酵工业, 2024 , 50(24) : 169 -175 . DOI: 10.13995/j.cnki.11-1802/ts.038426

Abstract

Limosilactobacillus fermentum FUA033, a strain capable of converting ellagic acid to urolithin A in vitro, has the potential to be developed as a next-generation probiotic.In the present study, the genome of the strain was sequenced with the PacBio Sequel and Illumina platform to have a comprehensive understanding of the genetic architecture of L.fermentum FUA033.The whole genome sequence was analyzed to evaluate the safety and probiotic potential of the strain.The chromosome size of this strain was 2 150 545 bp with a GC content of 51.34% and 2 215 protein-coding genes.No plasmid was identified.VFDB database and ARDB database annotation results showed that L.fermentum FUA033 contained 81 virulence genes and 9 resistance genes.Due to the absence of plasmids and transposons, it was tentatively concluded that the virulence factors and resistance genes would not transfer.19 acid tolerance genes (arcA, argH, napA, etc.), 16 bile salt tolerance genes (ldhA、ykpA、pstB1, etc.), 11 antioxidant genes (msrA, Trxs, ahpC, etc.), and 3 cell adhesion genes were mined from the genome sequence of L.fermentum FUA033.The results preliminarily revealed the safety and probiotic properties of L.fermentum FUA033 and laid a theoretical foundation for the development and further application of the strain.

Key words： Limosilactobacillus fermentum; urolithin A; genome sequencing; safety; probiotics

参考文献

[1] BAKKALBASI E, MENTES O, ARTIK N. Food ellagitannins-occurrence, effects of processing and storage[J]. Critical Reviews in Food Science and Nutrition, 2009, 49(3):283-298.
[2] GONZÁLEZ-SARRÁAS A, LARROSA M, TOMÍS-BARBERÁN F A, et al. NF-kappaB-dependent anti-inflammatory activity of urolithins, gut microbiota ellagic acid-derived metabolites, in human colonic fibroblasts[J]. British Journal of Nutrition, 2010, 104(4):503-512.
[3] LIU S, D’AMICO D, SHANKLAND E, et al.Effect of urolithin A supplementation on muscle endurance and mitochondrial health in older adults:A randomized clinical trial[J].JAMA Network Open, 2022, 5(1):e2144279.
[4] D’AMICO D, OLMER M, FOUASSIER A M, et al.Urolithin A improves mitochondrial health, reduces cartilage degeneration, and alleviates pain in osteoarthritis[J].Aging Cell, 2022, 21(8):e13662.
[5] WOJCIECHOWSKA O, KUJAWSKA M.Urolithin A in health and diseases:Prospects for Parkinson’s disease management[J].Antioxidants, 2023, 12(7):1479.
[6] JAYATUNGA D P W, HONE E, KHAIRA H, et al.Therapeutic potential of mitophagy-inducing microflora metabolite, urolithin A for Alzheimer′s disease[J].Nutrients, 2021, 13(11):3744.
[7] TOMÁS-BARBERÁN F A, GARCÍA-VILLALBA R, GONZÁLEZ-SARRÍAS A, et al. Ellagic acid metabolism by human gut microbiota: Consistent observation of three urolithin phenotypes in intervention trials, independent of food source, age, and health status[J]. Journal of Agricultural and Food Chemistry, 2014, 62(28):6535-6538.
[8] XIAN W Y, YANG S Y, DENG Y, et al.Distribution of urolithins metabotypes in healthy Chinese youth:Difference in gut microbiota and predicted metabolic pathways[J].Journal of Agricultural and Food Chemistry, 2021, 69(44):13055-13065.
[9] 肖玉欣, 王楠, 王婧, 等.鞣花酸和尿石素类代谢产物的生物活性及其对肠道健康的作用研究进展[J].食品科学, 2022, 43(9):275-284.
XIAO Y X, WANG N, WANG J, et al.A review of the bioactivity and gut health benefits of ellagic acid and its metabolites, urolithins[J].Food Science, 2022, 43(9):275-284.
[10] EVTYUGIN D D, MAGINA S, EVTUGUIN D V.Recent advances in the production and applications of ellagic acid and its derivatives.A review[J].Molecules, 2020, 25(12):2745.
[11] SELMA M V, TOMÁS-BARBERÁN F A, BELTRÁN D, et al. Gordonibacter urolithinfaciens sp. nov., a urolithin-producing bacterium isolated from the human gut[J]. International Journal of Systematic and Evolutionary Microbiology, 2014, 64(Pt 7):2346-2352.
[12] LIU Q T, LIU S, YE Q W, et al.A novel Streptococcus thermophilus FUA329 isolated from human breast milk capable of producing urolithin A from ellagic acid[J].Foods, 2022, 11(20):3280.
[13] MI H Y, LIU S, HAI Y, et al.Lactococcus garvieae FUA009, a novel intestinal bacterium capable of producing the bioactive metabolite urolithin A from ellagic acid[J].Foods, 2022, 11(17):2621.
[14] ZHANG X M, FANG Y W, YANG G, et al.Isolation and characterization of a novel human intestinal Enterococcus faecium FUA027 capable of producing urolithin A from ellagic acid[J].Frontiers in Nutrition, 2022, 9:1039697.
[15] ZHANG M W, CUI S M, MAO B Y, et al.Urolithin A produced by novel microbial fermentation possesses anti-aging effects by improving mitophagy and reducing reactive oxygen species in Caenorhabditis elegans [J].Journal of Agricultural and Food Chemistry, 2023, 71(16):6348-6357.
[16] 刘银辉, 余萍, 闵祥博, 等.具有分解嘌呤核苷能力的益生菌菌株筛选[J].中国微生态学杂志, 2023, 35(7):772-777.
LIU Y H, YU P, MIN X B, et al.Isolation of probiotic strains capable of decomposing purine nucleosides[J].Chinese Journal of Microecology, 2023, 35(7):772-777.
[17] LIM H J, LEE E H, YOON Y, et al.Portable Lysis apparatus for rapid single-step DNA extraction of Bacillus subtilis[J].Journal of Applied Microbiology, 2016, 120(2):379-387.
[18] GALPERIN M Y, MAKAROVA K S, WOLF Y I, et al.Expanded microbial genome coverage and improved protein family annotation in the COG database[J].Nucleic Acids Research, 2015, 43(Database issue):D261-D269.
[19] CANTAREL B L, COUTINHO P M, RANCUREL C, et al.The Carbohydrate-Active EnZymes database (CAZy):An expert resource for Glycogenomics[J].Nucleic Acids Research, 2009, 37(Database issue):D233-D238.
[20] BAIROCH A, APWEILER R.The SWISS-PROT protein sequence database and its supplement TrEMBL in 2000[J].Nucleic Acids Research, 2000, 28(1):45-48.
[21] CHEN L H, XIONG Z H, SUN L L, et al.VFDB 2012 update:Toward the genetic diversity and molecular evolution of bacterial virulence factors[J].Nucleic Acids Research, 2012, 40(Database issue):D641-D645.
[22] LIU B, POP M.ARDB antibiotic resistance genes database[J].Nucleic Acids Research, 2009, 37(Database issue):D443-D447.
[23] DENG L Q, LIU L M, FU T Y, et al.Genome sequence and evaluation of safety and probiotic potential of Lactiplantibacillus plantarum LPJZ-658[J].Microorganisms, 2023, 11(6):1620.
[24] 刘少文, 何光华, 王玲, 等.乳酸菌的应用及其安全性评价[J].中国畜牧业, 2018(11):53-56.
LIU S W, HE G H, WANG L, et al.Application of lactic acid bacteria and its safety evaluation[J].China Animal Industry, 2018(11):53-56.
[25] FRANCL A L, THONGARAM T, MILLER M J.The PTS transporters of Lactobacillus gasseri ATCC 33323[J].BMC Microbiology, 2010, 10:77.
[26] XIA M J, MU S T, FANG Y W, et al. Genetic and probiotic characteristics of urolithin A producing Enterococcus faecium FUA027[J]. Foods, 2023, 12(5):1021.
[27] 胡爱华, 敖晓琳, 陈岑, 等. 乳酸菌耐酸耐胆盐机制的研究进展[J]. 食品工业科技, 2015, 36(8):380-383; 389.
HU A H, AO X L, CHEN C, et al. Research progress on mechanism of lactic acid bacteria acid and bile salt resistance[J]. Science and Technology of Food Industry, 2015, 36(8):380-383; 389.
[28] 王崇民, 王翠竹. 可用于婴幼儿食品的“新食品原料”再添新成员: 长双歧杆菌长亚种BB536[J]. 食品安全导刊, 2022(22):8-9.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司