食品与发酵工业 >

2024 , Vol. 50 >Issue 24: 339 - 345

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.039617

分析与检测

超高效液相色谱-串联质谱法测定食用植物酵素中33种真菌毒素

  • 丁学妍 ,
  • 邵瑞婷 ,
  • 姜洁
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  • (北京市食品检验研究院,国家市场监管重点实验室(食品安全重大综合保障关键技术),北京,100041)
第一作者:学士,工程师(邵瑞婷高级工程师为通信作者,E-mail:rtshao@126.com)

收稿日期: 2024-04-18

  修回日期: 2024-05-22

  网络出版日期: 2024-12-30

基金资助

国家市场监督管理总局科技创新人才计划 (CXTD202307)

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Determination of 33 mycotoxins in edible plant source Jiaosu by ultra performance liquid chromatography tandem mass spectrometry

  • DING Xueyan ,
  • SHAO Ruiting ,
  • JIANG Jie
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  • (Beijing Institute of Food Inspection and Research, National Key Laboratory of Market Regulation (Key Technologies of Major Comprehensive Guarantee of Food Safety), Beijing 100041, China)

Received date: 2024-04-18

  Revised date: 2024-05-22

  Online published: 2024-12-30

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摘要

该实验基于超高效液相色谱-串联质谱法建立了一种准确、高效测定食用植物酵素中33种真菌毒素的分析方法。样品用溶剂[V(乙腈)∶V(水)∶V(甲酸)=79∶20∶1]提取,分散固相萃取(QuEChERS)净化,浓缩富集后用定容溶液复溶,样液经C18反相色谱柱(2.1 mm×100 mm,1.7 μm)分离,以乙腈和0.1%(体积分数)甲酸水溶液作为流动相梯度洗脱,采用电喷雾离子源(electrospray ionization, ESI) 多反应监测(multiple reaction monitoring, MRM)扫描模式,同时对正、负离子进行采集,阴性基质配制外标法定量。结果表明,33种真菌毒素在各自的线性范围内的决定系数(R2)均大于0.980,回收率为60.9%~119.4%,相对标准偏差为6.9%~14.9%。该方法具有前处理操作简单、定性定量准确、通量高等特点,可以用于食用植物酵素中33种真菌毒素的检测。

关键词： 食用植物酵素; QuEChERS净化; 真菌毒素

本文引用格式

丁学妍 , 邵瑞婷 , 姜洁 . 超高效液相色谱-串联质谱法测定食用植物酵素中33种真菌毒素[J]. 食品与发酵工业, 2024 , 50(24) : 339 -345 . DOI: 10.13995/j.cnki.11-1802/ts.039617

Abstract

A method for the simultaneous determination of 33 mycotoxins in edible plant source Jiaosu by ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was developed.The samples were extracted by acetonitrile-water-formic acid (79∶20∶1, by volume), and cleaned up by matrix dispersion solid phase extraction (QuEChERS).The analytes were separated by BEH C18 column (2.1 mm×100 mm,1.7 μm) using acetonitrile and 0.1% formic acid solution as mobile phase by gradient elution, analyzed in positive and negative electrospray ionization (ESI) mode by multiple reaction monitoring (MRM) mode, and quantified by matrix matching external standard method.Results showed that the linearities of 33 mycotoxins were good in respective ranges, with correlation coefficient (R2) greater than 0.980.The method recoveries were 60.9%-119.4%, and relative standard deviations (n=8) were 6.9%-14.9%.The method is simple and efficient for analysis of 33 mycotoxins in edible plant source Jiaosu.

Key words： edible plant source Jiaosu; QuEChERS purification; fusarium toxin

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