该研究探究了日粮中添加白藜芦醇对牛肌纤维类型和丝氨酸-苏氨酸蛋白激酶(serine-threonine protein kinase,Akt)/叉头盒蛋白O(forkhead box protein O,FoxO)通路信号分子的影响,并进一步以牛肌管细胞为研究对象,验证其分子机制。结果表明,肉牛日粮添加450 mg/kg白藜芦醇显著提高了背最长肌和腰大肌Ⅰ型纤维基因表达(P<0.05),降低了Ⅱ型纤维基因表达(P<0.05)。进一步分析其分子机制,发现,白藜芦醇显著上调肉牛背最长肌中磷酸酰肌醇3-激酶(phosphatidylinositol3-kinase,PI3K)和Akt的基因表达水平(P<0.05),显著下调FoxO3基因表达水平(P<0.05),显著上调了PI3K、p-Akt/Akt和p-FoxO1/FoxO1蛋白表达(P<0.05);显著上调肉牛腰大肌PI3K和Akt的基因表达水平(P<0.05),显著下调FoxO1和FoxO3基因表达水平(P<0.05),显著下调了FoxO1和FoxO3的蛋白表达水平(P<0.05),显著上调了PI3K、p-Akt、p-FoxO1/FoxO1和p-FoxO3/FoxO3蛋白表达(P<0.05),表明肉牛日粮添加白藜芦醇激活了Akt/FoxO通路。添加PI3K抑制剂渥曼青霉素(wortmannin)阻断Akt/FoxO信号通路后,白藜芦醇对牛肌管细胞中肌纤维类型的转化作用和对Akt/FoxO信号通路的激活作用被减弱。研究表明,白藜芦醇可通过激活Akt/FoxO通路促进牛肌纤维类型从Ⅱ型到Ⅰ型的转化。
In order to investigate the effects of dietary resveratrol supplementation on bovine muscle fiber types and serine-threonine protein kinase (Akt)/forkhead box protein O (FoxO) pathway related proteins and genes, this feeding experiment was undertaken.Furthermore, bovine myotubes were used to verify its deeply molecular mechanism.The results of muscle fiber types showed that dietary supplementation of 450 mg/kg resveratrol significantly increased the gene expression of type I fiber (P<0.05) and decreased the gene expression of type II fiber (P<0.05) in Longissimus dorsi and Psoas major muscle of beef cattle.Furtherly analysis of its molecular mechanism, the results showed that resveratrol significantly up-regulated the gene expression levels of phosphatidylinositol 3-kinase (PI3K) and Akt in beef cattle Longissimus dorsi muscle (P<0.05), significantly down-regulated the FoxO3 gene expression level (P<0.05), and significantly up-regulated the protein expressions of PI3K, p-Akt/Akt and p-FoxO1/FoxO1 (P<0.05).Additionally, dietary supplementation of resveratrol also significantly up-regulated the gene expression levels of PI3K and Akt in beef cattle Psoas major muscle (P<0.05), significantly decreased the gene expression levels of FoxO1 and FoxO3 (P<0.05), and significantly down-regulated the protein expression levels of FoxO1 and FoxO3 (P<0.05).At the same time, in resveratrol group, the protein expression levels of PI3K, p-Akt/Akt, p-FoxO1/FoxO1 and p-FoxO3/FoxO3 were significantly up-regulated (P<0.05), which indicated dietary supplementation of resveratrol could activate the Akt/FoxO pathway.PI3K inhibitors (wortmannin) were added to block the Akt/FoxO signaling pathway.Under this circumstance, the transformation of muscle fiber type and the activation of Akt/FoxO signal pathway in bovine myotubes were weakened by resveratrol.In conclusion, resveratrol can promote the transformation of bovine muscle fiber types from type Ⅱ to type I by activating the Akt/FoxO pathway.
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