该研究旨在探究乳清蛋白肽(whey hydrolyzed peptides,WHPs)对小鼠免疫功能的影响,以及乳清蛋白肽对过敏性鼻炎小鼠过敏症状的改善作用。研究分为两大部分,第一部分设置空白组(灌胃蒸馏水)、对照组(灌胃乳清蛋白溶液)、实验组(灌胃不同剂量的WHPs溶液),观察WHPs对小鼠免疫器官指数、特异性免疫和非特异性免疫的影响。第二部分设置空白组(正常小鼠,灌胃无菌蒸馏水)、模型对照组(过敏性鼻炎小鼠,灌胃无菌蒸馏水)、实验组(过敏性鼻炎小鼠、分别灌胃高、中、低剂量的WHPs),观察WHPs对小鼠过敏症状的影响。结果表明,WHPs干预可以显著增大小鼠足跖厚度(P＜0.05),上调白细胞介素-12(interleukin-12,IL-12)、干扰素-γ(interferon-γ,IFN-γ)等细胞因子水平(P＜0.05),增加免疫球蛋白IgA、IgM、IgG浓度(P＜0.05),增强巨噬细胞吞噬能力(P＜0.05)等,并且在增大小鼠足跖厚度、增加血清免疫球蛋白IgA、IgM浓度和增强巨噬细胞吞噬能力方面的作用显著优于乳清蛋白(P＜0.05)。对于过敏性鼻炎小鼠,WHPs可以显著降低血清和鼻腔灌洗液组胺水平(P＜0.05),有效调节IL-4、IL-12等炎症因子水平,减轻嗜酸性粒细胞浸润,并且其减轻嗜酸性粒细胞浸润效果优于乳清蛋白。WHPs对小鼠的免疫反应有显著的提高作用,对过敏性鼻炎小鼠过敏症状有一定的改善作用,并且WHPs较乳清蛋白对免疫功能的提高和过敏性鼻炎的改善作用更加显著。
This study aimed to investigate the immunomodulatory effect of whey hydrolyzed peptides (WHPs) on mice and the therapeutic effect of WHPs on allergic rhinitis in mice.The research was separated into two sections.In the first part, mice’s immune organ index, specific immunity, and non-specific immunity were studied using a blank group (distilled water), a control group (whey protein solution), and an experimental group (WHPs solution with varying dosages).In the second part, mice were divided into five groups, including a blank group (normal mice were given aseptic distilled water), a model control group (mice with allergic rhinitis were given aseptic distilled water), and experimental groups (mice with allergic rhinitis were given high, medium, and low doses of WHPs respectively).Results showed that WHPs intervention significantly increased the degree of foot swelling of the mice (P＜0.05), the serum levels of interleukin-12 (IL-12) and interferon-γ (IFN-γ) (P＜0.05), the levels of immunoglobulin IgA, IgM, and IgG concentrations (P＜0.05), and also increased the activity of macrophages (P＜0.05).Moreover, it was significantly superior to whey protein in increasing the thickness of mouse feet, increasing the concentration of immunoglobulin IgA and IgM, and enhancing the phagocytosis ability of macrophages.WHPs may significantly decrease histamine levels in blood and nasallavage fluid in allergic rhinitis mice (P＜0.05), effectively control IL-4, IL-12, and other inflammatory factors, and descrese the counts of eosinophilic.It was more effective than whey protein in decreasing the counts of eosinophilic.WHPs significantly increased the immunological response of mice as well as the allergy symptoms of mice with allergic rhinitis, and WHPs was more effective than whey protein in improving immune function and allergic rhinitis.
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