为开发能应用于复杂的食品基质中且准确高、灵敏高、特异性好的黄曲霉毒素B1(aflatoxins B1,AFB1)检测方法,该研究以抗体偶联的磁性纳米微球(MMPMs- AFB1抗体)为捕获探针,包被原/葡萄糖氧化酶(glucose oxidase,GOx)偶联的纳米微球(AFB1-OVA-PPs-GOx)为信号探针,结合芬顿反应-硫磺素T荧光核酸探针体系开发了一种“Turn on”型AFB1磁分离荧光免疫分析方法。结果显示,该方法对AFB1的检测范围为0.05 ~50 ng/mL,最低检测限为0.027 ng/mL。在AFB1的添加质量浓度为1、5、20、200 ng/g(ng/mL)的加标植物源性食品样品中,AFB1的回收率良好,为81.6%~112.1%,变异系数值为1.2%~15.8%。该方法成功应用于玉米粉质控样品中AFB1的检测。结果表明,所建立的方法灵敏度高,特异性好和抗基质干扰能力强,可用于食品中的AFB1的精确定量检测。
To develop an accurate, sensitive, and specific method for the detection of aflatoxins B1(AFB1) in complex food matrix, antibody modified magnetic nano-microspheres (MMPMs-AFB1 antibody) was used as the capture probes, and antigen/GOx modified nano-microspheres (AFB1-OVA-PPs-GOx) was used as the signal probes, combining with Fenton reaction-sulfur T (ThT) fluorescent nucleic acid probe system, a “turn on” type AFB1 magnetic separation fluorescence immunoassay method was developed.The detection range of AFB1by this method was 0.05-50 ng/mL and the detection limit was 0.027 ng/mL.The concentration of the spiked sample was 1, 5, 20, 200 ng/g(ng/mL), the recovery of AFB1 in spiked foodstuffs ranged from 81.6% to 112.1%, with the coefficient of variation of 1.2%-15.8%.In quality control sample of corn flour, the method was successfully applied to detect AFB1.This method has high sensitivity, good specificity and strong anti-matrix interference ability, and can be used for accurate quantitative detection of AFB1 in food.
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