食品与发酵工业 >

2025 , Vol. 51 >Issue 9: 50 - 58

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.039507

研究报告

白酒窖泥格氏乳球菌的比较基因组分析

  • 赵倬儒 ,
  • 陈晓松 ,
  • 陈聪 ,
  • 邹伟
展开
  • 1(四川轻化工大学 生物工程学院,四川 宜宾,644005)
    2(四川轻化工大学,浓香型白酒资源微生物开发与大数据应用研究室,四川 宜宾,644005)
第一作者:硕士研究生(邹伟副教授为通信作者,E-mail:weizou@suse.edu.cn)

收稿日期: 2024-04-09

  修回日期: 2024-05-08

  网络出版日期: 2025-05-28

基金资助

泸州老窖研究生创新基金项目(LJCX2022-2);四川轻化工大学研究生教育教学改革研究项目(JG202316);四川轻化工大学研究生创新基金资助项目(Y2023234)

收起

Comparative genomic analysis of Lactococcus garvieae in pit mud of Baijiu

  • ZHAO Zhuoru ,
  • CHEN Xiaosong ,
  • CHEN Cong ,
  • ZOU Wei
Expand
  • 1(College of Bioengineering, Sichuan University of Science & Engineering, Yibin 644005, China)
    2(Lab of Baijiu Resource Microorganisms & Big Data, Sichuan University of Science & Engineering, Yibin 644005, China)

Received date: 2024-04-09

  Revised date: 2024-05-08

  Online published: 2025-05-28

Fold

摘要

格氏乳球菌(Lactococcus garvieae)是一种广泛存在于白酒酿造生态系统的功能微生物,在发酵过程中不仅能产生多种风味物质,还能在维持酿造体系的生态平衡中起关键作用。该研究对4株窖泥环境L.garvieae进行功能基因注释,并结合其他生态位L.garvieae进行泛基因组分析,探究其系统发育中的群体特征及白酒环境下的个体特异性。结果表明L.garvieae的泛基因组表现出开放的状态,核心基因组主要涉及菌体的基础代谢功能。核心基因组KEGG注释表明L.garvieae基因组均能够产生促进微生物生长的纤维素酶、糖化酶、酯酶,同时产生乳酸、乙酸、乙醇等前体物质,通过酯酶催化生成乳酸乙酯和乙酸乙酯。毒力因子分析评估窖泥环境的4株L.garvieae在自身基因水平上具有安全性,但也有可能会受到外界调控。L.garvieae中存在大量的水平基因转移。该研究相关结果揭示了L.garvieae遗传多样性和进化特征,为其在白酒行业中的应用提供了一定理论依据。

关键词： 格氏乳球菌; 泛基因组分析; 乳酸菌; 核心基因组; 白酒

本文引用格式

赵倬儒 , 陈晓松 , 陈聪 , 邹伟 . 白酒窖泥格氏乳球菌的比较基因组分析[J]. 食品与发酵工业, 2025 , 51(9) : 50 -58 . DOI: 10.13995/j.cnki.11-1802/ts.039507

Abstract

The Lactococcus garvieae is a functionally significant microorganism widely present in the Baijiu brewing ecosystem.It not only produces diverse flavor compounds during fermentation but also plays a pivotal role in maintaining the ecological balance of the brewing system. The study annotated the functional genes of four strains of L.garvieae isolated from pit mud environments, conducted a pan-genome analysis in combination with other ecological niches of L.garvieae to explore their population characteristics in phylogenetic development and individual specificities in the Baijiu environment.The results revealed an open state of the L.garvieae pan-genome, with the core genome primarily involved in basic metabolic functions of the bacteria.The KEGG annotation of the core genome demonstrates that the L.garvieae genome possesses the ability to produce cellulases, glycosidases, and esterases, enzymes that facilitate microbial growth.Additionally, it generated precursor substances, including lactic acid, acetic acid, and ethanol.These precursors were catalyzed by esterase activity, resulting in the formation of lactic ethyl ester and acetic ethyl ester.Virulence factor analysis indicated that the four strains of L.garvieae isolated from pit mud environment possess safety at the genomic level, but may be potentially influenced by external regulatory factors.Substantial horizontal gene transfer was observed in L.garvieae.The findings of this study reveal the genetic diversity and evolutionary characteristics of L.garvieae, providing a theoretical basis for its application in the Baijiu industry.

Key words： Lactococcus garvieae; pan-genome analysis; lactic acid bacteria; core genome; Baijiu

参考文献

[1] LIN Y S, KWEH K H, KOH T H, et al.Genomic analysis of Lactococcus garvieae isolates[J].Pathology, 2020, 52(6):700-707.
[2] 阎春悦, 利佳炜, 张茜, 等.不同香型白酒酿造过程乳酸菌的研究进展[J].酿酒科技, 2023(9):114-120.
YAN C Y, LI J W, ZHANG Q, et al.Research progress in lactic acid bacteria in the production of Baijiu of different flavor types[J].Liquor-Making Science & Technology, 2023(9):114-120.
[3] 吴正坤, 缪礼鸿, 张明春, 等.不同贮存期高温大曲中乳酸菌的多样性及其耐受性分析[J].中国酿造, 2018, 37(6):61-67.
WU Z K, MIAO L H, ZHANG M C, et al.Diversity and tolerance of lactic acid bacteria in high temperature Daqu at different storage periods[J].China Brewing, 2018, 37(6):61-67.
[4] 刘凡, 仇钰莹, 周新虎, 等.洋河浓香型白酒酒醅中产酸细菌与有机酸合成的相关性研究[J].食品与发酵工业, 2018, 44(12):22-29.
LIU F, QIU Y Y, ZHOU X H, et al.The correlation between organic acid producing bacteria and organic acids biosynthesis in fermented grains of Yanghe strong-aroma spirit[J].Food and Fermentation Industries, 2018, 44(12):22-29.
[5] 胡世伟, 王超凡, 王景春, 等.浓香型白酒窖泥微生物群落及其风味成分研究进展[J].中国酿造, 2023, 42(12):9-12.
HU S W, WANG C F, WANG J C, et al.Research progress of microbial community and flavor substances in strong-flavor Baijiu pit mud[J].China Brewing, 2023, 42(12):9-12.
[6] 陈聪, 邹伟, 汤秀娟, 等.产酯酶格氏乳球菌的筛选、鉴定与基因组注释[J].食品科学, 2024, 45(8):87-95.
CHEN C, ZOU W, TANG X J, et al.Screening, identification and genome annotation of esterase-producing Lactococcus garvieae[J].Food Science, 2024, 45(8):87-95.
[7] 骆红波, 孙优兰, 范奇高, 等.组学技术在白酒酿造微生物中的应用进展[J].中国酿造, 2023, 42(12):13-21.
LUO H B, SUN Y L, FAN Q G, et al.Progress on the application of omics technologies in Baijiu brewing microorganism[J].China Brewing, 2023, 42(12):13-21.
[8] ZHOU X G, REN L F, LI Y T, et al.The next-generation sequencing technology:A technology review and future perspective[J].Science China.Life Sciences, 2010, 53(1):44-57.
[9] 余晶晶, 黄永光.基因组学及其在白酒酿造基础研究领域中的应用[J].食品与发酵工业, 2021, 47(7):278-284.
YU J J, HUANG Y G.Genomics and its application in basic research field of Baijiu brewing[J].Food and Fermentation Industries, 2021, 47(7):278-284.
[10] TETTELIN H, MASIGNANI V, CIESLEWICZ M J, et al.Genome analysis of multiple pathogenic isolates of Streptococcus agalactiae:Implications for the microbial “pan-genome”[J].Proceedings of the National Academy of Sciences of the United States of America, 2005, 102(39):13950-13955.
[11] 何苗, 赵雨晴, 李居行, 等.弯曲乳杆菌与清酒乳杆菌的比较基因组学分析[J].食品科学, 2024, 45(2):48-56.
HE M, ZHAO Y Q, LI J X, et al.Comparative genomic analysis of Latilactobacillus curvatus and L.sakei[J].Food Science, 2024, 45(2):48-56.
[12] TATUSOVA T, DICUCCIO M, BADRETDIN A, et al.NCBI prokaryotic genome annotation pipeline[J].Nucleic Acids Research, 2016, 44(14):6614-6624.
[13] AZIZ R K, BARTELS D, BEST A A, et al.The RAST Server:Rapid annotations using subsystems technology[J].BMC Genomics, 2008, 9:75.
[14] CHAUDHARI N M, GUPTA V K, DUTTA C.BPGA- an ultra-fast pan-genome analysis pipeline[J].Scientific Reports, 2016, 6:24373.
[15] ZHAO Y B, JIA X M, YANG J H, et al.PanGP:A tool for quickly analyzing bacterial pan-genome profile[J].Bioinformatics, 2014, 30(9):1297-1299.
[16] RICHTER M, ROSSELLÓ-MÓRA R, OLIVER GLÖCKNER F, et al.JSpeciesWS:A web server for prokaryotic species circumscription based on pairwise genome comparison[J].Bioinformatics, 2016, 32(6):929-931.
[17] KUMAR S, STECHER G, LI M, et al.MEGA X:Molecular evolutionary genetics analysis across computing platforms[J].Molecular Biology and Evolution, 2018, 35(6):1547-1549.
[18] KANEHISA M, SATO Y, KAWASHIMA M, et al.KEGG as a reference resource for gene and protein annotation[J].Nucleic Acids Research, 2016, 44(D1):D457-D462.
[19] HUERTA-CEPAS J, SZKLARCZYK D, HELLER D, et al.eggNOG 5.0:A hierarchical, functionally and phylogenetically annotated orthology resource based on 5090 organisms and 2502 viruses[J].Nucleic Acids Research, 2019, 47(D1):D309-D314.
[20] CHEN L H, YANG J, YU J, et al.VFDB:A reference database for bacterial virulence factors[J].Nucleic Acids Research, 2005, 33(Database issue):D325-D328.
[21] BERTELLI C, LAIRD M R, WILLIAMS K P, et al.IslandViewer 4:Expanded prediction of genomic islands for larger-scale datasets[J].Nucleic Acids Research, 2017, 45(W1):W30-W35.
[22] ARNDT D, GRANT J R, MARCU A, et al.PHASTER:A better, faster version of the PHAST phage search tool[J].Nucleic Acids Research, 2016, 44(W1):W16-W21.
[23] PARKS D H, IMELFORT M, SKENNERTON C T, et al.CheckM:Assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes[J].Genome Research, 2015, 25(7):1043-1055.
[24] ALIKHAN N F, PETTY N K, BEN ZAKOUR N L, et al.BLAST ring image generator (BRIG):Simple prokaryote genome comparisons[J].BMC Genomics, 2011, 12:402.
[25] MORIYA Y, ITOH M, OKUDA S, et al.KAAS:An automatic genome annotation and pathway reconstruction server[J].Nucleic Acids Research, 2007, 35(Web Server issue):W182-W185.
[26] BOBER M, MÖRGELIN M, OLIN A I, et al.The membrane bound LRR lipoprotein Slr, and the cell wall-anchored M1 protein from Streptococcus pyogenes both interact with type I collagen[J].PLoS One, 2011, 6(5):e20345.
[27] XU S Y, LIU Y, GAO J, et al.Comparative genomic analysis of Streptococcus dysgalactiae subspecies dysgalactiae isolated from bovine mastitis in China[J].Frontiers in Microbiology, 2021, 12:751863.
[28] 贾伟娟, 宋丽丽, 张玲艳, 等.蜡样芽胞杆菌毒素的最新研究进展[J].中国抗生素杂志, 2022, 47(6):534-542.
JIA W J, SONG L L, ZHANG L Y, et al.Research progress of the toxins from Bacillus cereus[J].Chinese Journal of Antibiotics, 2022, 47(6):534-542.
[29] HAAS W, SHEPARD B D, GILMORE M S.Two-component regulator of Enterococcus faecalis cytolysin responds to quorum-sensing autoinduction[J].Nature, 2002, 415(6867):84-87.
[30] SEBAIHIA M, PECK M W, MINTON N P, et al.Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes[J].Genome Research, 2007, 17(7):1082-1092.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司