研究报告

开菲尔源酵母对开菲尔乳杆菌生长代谢的影响

  • 黎世威 ,
  • 白英
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  • (内蒙古农业大学 食品科学与工程学院, 内蒙古自治区 呼和浩特, 010018)
第一作者:硕士研究生(白英教授为通信作者,E-mail:baiying77@sina.com)

收稿日期: 2024-03-22

  修回日期: 2024-05-23

  网络出版日期: 2025-05-28

基金资助

国家自然科学基金项目(31760460);内蒙古自治区科技项目(2023YFHH0121)

Influence of Kefir yeast on growth and metabolism of Lactobacillus kefiri

  • LI Shiwei ,
  • BAI Ying
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  • (School of Food Science and Engineering, Inner Mongolia Agriculture University, Hohhot 010018, China)

Received date: 2024-03-22

  Revised date: 2024-05-23

  Online published: 2025-05-28

摘要

该文研究了对分离于开菲尔中的一株高产胞外多糖乳酸菌Lactobacillus kefiri(L63)和开菲尔源不同的2株酵母的相互作用。通过qPCR扩增L63的特征序列来确定其单独生长与共培养时的生长状态,发现克鲁维酵母Y4在前期可促进其生长,而在发酵后期2株酵母均抑制其生长;通过苯酚硫酸法测定其共培养时和单独生长时产胞外多糖的差异,发现与2株酵母共培养均能显著提升其胞外多糖产量(P<0.01);而在共同发酵脱脂乳时,发现与2株酵母共培养时在发酵前期可显著提升发酵乳的酸度,而在后期与L63单独发酵结果趋于相同,代谢组学检测结果显示,共培养时的差异代谢物主要分布在细胞生长、能量代谢、呼吸作用等通路,这些都与细胞的生长状态密切相关,在一定程度上解释了L63共培养时生长状态变化的原因。

本文引用格式

黎世威 , 白英 . 开菲尔源酵母对开菲尔乳杆菌生长代谢的影响[J]. 食品与发酵工业, 2025 , 51(9) : 67 -75 . DOI: 10.13995/j.cnki.11-1802/ts.039320

Abstract

This study investigated the interactions between a high-exopolysaccharide-producing Lactobacillus kefiri (L63) isolated from kefir and two different yeasts derived from kefir.Using qPCR to amplify characteristic sequences of L63, the growth status during monoculture and coculture was determined.Results revealed that Kluyveromyces marxianus Y4 initially promoted the growth of L63, while both yeasts inhibited its growth in the later stages of fermentation.The phenol-sulfuric acid method was employed to measure differences in exopolysaccharide (EPS) production during monoculture and coculture.It was observed that coculturing with either yeast significantly increased EPS production (P<0.01).During the co-fermentation of skim milk, it was found that coculturing with both yeasts significantly increased acidity in the early fermentation stage, while in the later stage, results tended to align with a monoculture of L63.Metabolomic analysis revealed differential metabolite distribution during coculture, primarily associated with cell growth, energy metabolism, and respiratory processes, closely linked to the growth status of the cells, thereby partially elucidating the reasons for the observed changes in growth status during coculture of L63.

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