食品与发酵工业 >

2025 , Vol. 51 >Issue 9: 152 - 159

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.039673

研究报告

水溶性酵母β-葡聚糖的制备及体外抗氧化、降糖活性研究

  • 李赛芬 ,
  • 曹桦强 ,
  • 李涵 ,
  • 赵晨晨 ,
  • 张彭湃
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  • 1(河南大学 生命科学学院,河南 开封,475004)
    2(河南省应用微生物工程研究中心,河南 开封,475004)
第一作者:硕士研究生(张彭湃副教授为通信作者,E-mail:bio_apai@163.com)

收稿日期: 2024-04-24

  修回日期: 2024-06-06

  网络出版日期: 2025-05-28

基金资助

开封市科技攻关计划项目(2302007)

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Preparation and in vitro antioxidant and hypoglycemic activities of water-soluble yeast β-glucan

  • LI Saifen ,
  • CAO Huaqiang ,
  • LI Han ,
  • ZHAO Chenchen ,
  • ZHANG Pengpai
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  • 1(School of Life Sciences, Henan University, Kaifeng 475004, China)
    2(Engineering Research Center for Applied Microbiology of Henan Province, Kaifeng 475004, China)

Received date: 2024-04-24

  Revised date: 2024-06-06

  Online published: 2025-05-28

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摘要

以酿酒酵母细胞壁为原料,采用二甲基亚砜(dimethyl sulfoxide,DMSO)溶解-乙醇沉淀法,通过响应面分析法确定制备水溶性酵母β-D-葡聚糖(water-soluble yeast β-D-glucan,WSYG)的最佳工艺条件,利用高效液相色谱、气相色谱、红外光谱和刚果红试验等方法探究WSYG的理化性质,并通过体外试验测试其抗氧化及降血糖活性。结果表明,DMSO溶解-乙醇沉淀法制备WSYG的最佳工艺条件为体积分数90%的DMSO,体积分数91%的乙醇,乙醇溶液与DMSO溶液体积比4.4∶1,水溶性多糖得率为(16.38±0.17)%,多糖含量为(96.02±1.37)%。多糖的相对分子质量为602.56 kDa,单糖组成分析表明其由D-葡萄糖组成,红外光谱图显示其具有多糖的特征吸收峰,并呈β构型;原子力显微镜和刚果红试验显示其在水溶液中为链状且不具有三螺旋结构,这可能是酵母β-葡聚糖水溶性增加的原因。体外活性测试显示WSYG具有优良的超氧阴离子清除能力,且对α-淀粉酶、α-葡萄糖苷酶具有抑制效果,表现出一定的降血糖活性。该方法简便易行,所得的WSYG纯度高、活性较好,可为酵母β-葡聚糖的进一步开发和利用提供理论依据。

关键词： 酵母细胞壁; 水溶性β-葡聚糖; 抗氧化活性; 降血糖活性

本文引用格式

李赛芬 , 曹桦强 , 李涵 , 赵晨晨 , 张彭湃 . 水溶性酵母β-葡聚糖的制备及体外抗氧化、降糖活性研究[J]. 食品与发酵工业, 2025 , 51(9) : 152 -159 . DOI: 10.13995/j.cnki.11-1802/ts.039673

Abstract

In this study, an effective protocol for the preparation of water-soluble yeast β-D-glucan (WSYG) was established.By means of dimethyl sulfoxide(DMSO) extraction and ethanol precipitation, WSYG was prepared from brewer's yeast cell wall.The optimal process conditions were determined by response surface analysis.Besides, the physicochemical properties of WSYG were investigated using HPLC, GC, FT-IR, and Congo red test.And its antioxidant and hypoglycemic activities were evaluated through in vitro experiments.The results indicated that the maximum yield of WSYG was (16.38±0.17)% and the polysaccharide content was (96.02±1.37)%, using the DMSO-dissolved ethanol precipitation method with the conditions of 90% (v/v) DMSO concentration, 91% (v/v) ethanol concentration, and a 4.4∶1 volume ratio of ethanol to DMSO solution.The relative molecular mass of the polysaccharide was 602.56 kDa, and monosaccharide composition analysis showed that it consisted solely of D-glucose, and the infrared spectrogram displayed characteristic absorption peaks of polysaccharides with a β-configuration.Furthermore, the atomic force microscopy and the Congo red test demonstrated that it existed in a chain form and did not possess a triple helix structure in aqueous solution, which might contribute to the increased water-solubility of yeast β-glucan.The in vitro activity assays revealed that WSYG had excellent superoxide anion scavenging ability and inhibitory effects on α-amylase and α-glucosidase, exhibiting certain hypoglycemic activity.This approach is facile and practical, and the resulting WSYG demonstrates high purity and good activity.The findings of this study can provide a theoretical basis for the further development and utilization of yeast β-glucan.

Key words： yeast cell wall; water-soluble β-glucan; antioxidant activity; hypoglycemic activity

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