表没食子儿茶素没食子酸酯[(-)-epigallocatechin-3-gallate,EGCG]具有抗氧化、抗炎、抗肿瘤和保护心脏等生理功能,但稳定性差及在消化前期过快降解等使其应用受限。该研究利用油包水(water in oil,W/O)乳液模板在内水相制备微囊,构建具有囊壳结构的W/O高内相乳液(microcapsule-shell structured W/O high internal phase emulsions,MS-W/O HIPEs),利用冷冻扫描电镜、流变仪、模拟体外消化和动物实验对W/O HIPEs结构、负载EGCG消化特性与其对小鼠结肠炎的影响进行表征。与W/O HIPEs和蜂蜡-W/O HIPEs相比,MS-W/O HIPEs具有更高凝胶强度。负载EGCG的MS-W/O HIPEs在肠消化后的保留率约为EGCG溶液的14倍,显著减缓了EGCG在消化过程中的降解速率。与EGCG溶液相比,负载EGCG的MS-W/O HIPEs能显著改善小鼠体重减轻、结肠红肿和缩短等症状,有效降低促炎因子TNF-α、IL-1β与IL-6的表达并促进抗炎因子IL-10的产生。综上,利用W/O乳液模板在内水相制备微囊,构建MS-W/O HIPEs是实现EGCG结肠递送的可靠技术,为EGCG相关产品的开发奠定了理论基础。
(-)-Epigallocatechin-3-gallate (EGCG) exhibits physiological functions such as antioxidation, anti-inflammation, anti-tumor properties, and cardiac protection.However, its application is limited due to poor stability and rapid degradation during the early stages of digestion.This study utilized a water-in-oil (W/O) emulsion template to prepare microcapsules in the internal aqueous phase, creating a microcapsule-shell structured W/O high internal phase emulsions (MS-W/O HIPEs).The structure of W/O HIPEs, the digestion characteristics of the loaded EGCG, and their effects on mouse colitis were characterized using Cryo-scanning electron microscopy, rheometry, simulated in vitro digestion, and animal experiments.Compared to W/O HIPEs and BW-W/O HIPEs, MS-W/O HIPEs exhibited higher gel strength.The encapsulated EGCG in MS-W/O HIPEs retained about 14 times more EGCG post-intestinal digestion than the EGCG solution, significantly slowing down the degradation of EGCG during digestion.Compared to the EGCG solution, the MS-W/O HIPEs loaded with EGCG significantly improved symptoms in mice such as weight loss, colon redness, and shortening, effectively reducing the expression of pro-inflammatory factors TNF-α, IL-1β, and IL-6, and promoted the production of the anti-inflammatory factor IL-10.In summary, using a W/O emulsion template to prepare microcapsules in the internal aqueous phase to construct MS-W/O HIPEs is a reliable technique for colonic delivery of EGCG, laying a theoretical foundation for the development of EGCG-related products.
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