食品与发酵工业 >

2025 , Vol. 51 >Issue 9: 230 - 238

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040074

研究报告

卵黄高磷蛋白磷酸肽-锌螯合物的制备及其结构表征

  • 张铭冉 ,
  • 薛天睿 ,
  • 吕彬斐 ,
  • 李灿 ,
  • 张晓维
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  • (天津科技大学 食品科学与工程学院,天津,300457)
第一作者:硕士研究生(张晓维副教授为通信作者,E-mail:zhangxw@tust.edu.cn)

收稿日期: 2024-06-03

  修回日期: 2024-07-23

  网络出版日期: 2025-05-28

基金资助

天津市教委科研计划项目(2019KJ204)

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Preparation and structural characterization of phosvtin phosphopeptide peptide-zinc chelation

  • ZHANG Mingran ,
  • XUE Tianrui ,
  • LYU Binfei ,
  • LI Can ,
  • ZHANG Xiaowei
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  • (College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China)

Received date: 2024-06-03

  Revised date: 2024-07-23

  Online published: 2025-05-28

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摘要

通过酶解得到卵黄高磷蛋白磷酸肽(phosvitin phosphopeptide,PPP),并与锌螯合制备卵黄高磷蛋白磷酸肽-锌螯合物(phosvitin phosphopeptide-zinc chelate,PPP-Zn),从而开发安全、易吸收的锌补充剂。卵黄高磷蛋白先经碱性蛋白酶和胰蛋白酶两步酶解,再经α-糜蛋白酶进行三步酶解,以水解度为指标优化其酶解条件以高效制备PPP。以锌螯合率为指标,利用响应面试验优化PPP-Zn的制备工艺。采用Zeta电位、荧光光谱、傅里叶红外光谱和扫描电镜等方法对PPP-Zn进行结构表征。结果表明,PPP最佳制备条件为pH 7.50、酶解4 h、温度50 ℃以及添加8%的α-糜蛋白酶,此时水解度为(26.62±0.39)%。PPP-Zn的最佳制备条件为肽锌比1∶90(g∶mmol)、pH 5.20、温度60 ℃以及时间60 min,此时锌螯合率达(94.18±0.38)%。螯合锌后,PPP-Zn的Zeta电位和荧光强度均降低,红外光谱表明PPP中的羧基氧原子、氨基氮原子以及磷酸基团是锌离子的主要螯合位点。PPP-Zn优异的载锌量表明其极有作为新型的锌补充剂的潜力。

关键词： 卵黄高磷蛋白磷酸肽; 肽-锌螯合物; 工艺优化; 结构表征

本文引用格式

张铭冉 , 薛天睿 , 吕彬斐 , 李灿 , 张晓维 . 卵黄高磷蛋白磷酸肽-锌螯合物的制备及其结构表征[J]. 食品与发酵工业, 2025 , 51(9) : 230 -238 . DOI: 10.13995/j.cnki.11-1802/ts.040074

Abstract

The phosvitin phosphopeptide (PPP) was obtained through enzymatic hydrolysis of phosvitin (PV) and subsequently chelated with zinc to produce the phosvitin phosphopeptide-zinc chelate (PPP-Zn), aiming to develop safe and highly bioavailable zinc supplements.PV was initially subjected to a two-step enzymatic hydrolysis using alkaline protease and trypsin, followed by a third step involving α-chymotrypsin.The conditions for enzymatic hydrolysis were optimized based on the degree of hydrolysis to prepare PPP effectively.The preparation process of PPP-Zn was further optimized using response surface methodology, with the zinc chelation rate as an index parameter.The structure of PPP-Zn was characterized using Zeta potential analysis, fluorescence spectroscopy, Fourier transform infrared spectroscopy, and scanning electron microscopy.Results showed that the optimal conditions for preparing PPP were pH 7.50 and enzymolysis at 50 ℃ for 4 h with an addition of 8% α-chymotrypsin, resulting in a hydrolysis degree of (26.62±0.39)%.For PPP-Zn preparation, the optimum conditions included a mass ratio of zinc to peptide at 1∶90(g∶mmol), pH 5.20, and temperature at 60 ℃ for 60 min, achieving a zinc chelation rate of (94.18±0.38)%.Upon zinc chelation, both Zeta potential and fluorescence intensity decreased while FTIR spectra indicated that carboxyl oxygen atoms, amino nitrogen atoms, and phosphate groups within PPP served as primary sites for binding with zinc ions.The high content of zinc in PPP-Zn indicated its promising potential as a novel type of effective zinc supplement.

Key words： phosvitin phosphopeptide; peptide-zinc chelate; process optimization; structural characterization

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