食品与发酵工业 >

2025 , Vol. 51 >Issue 8: 241 - 248

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040088

研究报告

负载柠檬苦素的双蛋白高内相乳液表征及其稳定性研究

  • 梁鑫富 ,
  • 曾泽政 ,
  • 戴梓茹 ,
  • 蔡秋杏 ,
  • 董庆亮
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  • 1(北部湾大学 食品工程学院,广西 钦州,535000)
    2(广西高校北部湾海产品高值化利用与预制食品重点实验室,广西 钦州,535000)
    3(广西大学 轻工与食品工程学院,广西 南宁,530000)
第一作者:硕士研究生(董庆亮副教授为通信作者,E-mail:dql80@163.com)

收稿日期: 2024-06-03

  修回日期: 2024-07-02

  网络出版日期: 2025-05-28

基金资助

广西科技计划项目(桂科AD20159019);北部湾大学高层次人才科研启动经费项目(2019KYQD10)

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Characterization and stability of limonin-loaded double-protein high intrinsic emulsions

  • LIANG Xinfu ,
  • ZENG Zezheng ,
  • DAI Ziru ,
  • CAI Qiuxing ,
  • DONG Qingliang
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  • 1(Food Engineering College, Beibu Gulf University, Qinzhou 535000, China)
    2(Guangxi College and University Key Laboratory of High-value Utilization of Seafood and Prepared Food in Beibu Gulf, Qinzhou 535000, China)
    3(College of Light Industry and Food Engineering, Guangxi University, Nanning 530000, China)

Received date: 2024-06-03

  Revised date: 2024-07-02

  Online published: 2025-05-28

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摘要

柠檬苦素具有多种生理活性,但结构稳定性差,限制了柠檬苦素的广泛应用。为了提高柠檬苦素的结构稳定性,该文以乳清蛋白-玉米醇溶蛋白复合纳米颗粒为高内相乳液的稳定剂,制备负载柠檬苦素的高内相乳液,并对该乳液进行表征,进一步探究乳液负载对柠檬苦素结构稳定性的影响。结果表明,随着玉米醇溶蛋白的质量增加,负载柠檬苦素的双蛋白复合物高内相乳液粒径呈现先减小后增大的趋势,激光共聚焦显微镜结果也证实了这一粒径变化规律,乳液D(2,3)和D(4,3)最小值分别为(13.55±2.02) μm和(136.13±0.93) μm;该乳液呈现假塑性流动行为,并形成了以弹性为主的凝胶状网络结构;此外,该乳液还具有良好的热稳定性、光照稳定性和贮藏稳定性,而柠檬苦素经该高内相乳液负载后,热处理和光照处理下的最高保留率分别为(93.46±0.68)%和(76.47±0.95)%,提高了柠檬苦素的热稳定性和光照稳定性。该研究结果可为进一步开发和应用柠檬苦素提供理论依据。

关键词： 柠檬苦素; 双蛋白纳米颗粒; 高内相乳液; 稳定性

本文引用格式

梁鑫富 , 曾泽政 , 戴梓茹 , 蔡秋杏 , 董庆亮 . 负载柠檬苦素的双蛋白高内相乳液表征及其稳定性研究[J]. 食品与发酵工业, 2025 , 51(8) : 241 -248 . DOI: 10.13995/j.cnki.11-1802/ts.040088

Abstract

Limonin has a variety of physiological activities, but its poor structural stability limits the wide application of limonin.To improve the structural stability of limonin, a high intrinsic emulsion loaded with limonin was prepared using whey protein-zein composite nanoparticles as stabilizers for high intrinsic emulsions, and the emulsions were characterized to further explore the effect of emulsion loading on the structural stability of limonin.Results showed that with the increase of the mass of zein, the particle size of the high intrinsic emulsions of the two-protein complex loaded with limonin showed a trend of first decreasing and then increasing, the results of laser confocal microscopy also confirmed the variation of particle size, and the minimum values of D(2,3) and D(4,3) of the emulsion were (13.55±2.02) μm and (136.13±0.93) μm, respectively.The emulsion exhibited a pseudoplastic flow behavior and formed a gel-like network structure dominated by elasticity.In addition, the emulsion also had good thermal stability, light stability, and storage stability, and the highest retention rates of limonin under heat treatment and light treatment were (93.46±0.68)% and (76.47±0.95)%, respectively, which improved the thermal stability and light stability of limonin.The results of this study can provide a theoretical basis for the further development and application of limonin.

Key words： limonin; double-protein nanoparticles; high intrinsic emulsion; stability

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