该研究主要探讨红茶干预小鼠胃组织和胃内容物代谢的差异变化,分析其对饱、空腹小鼠胃生物节律的影响。采用红茶对6周龄C57BL/6饱、空腹小鼠分别进行生理盐水和红茶提取物灌胃(450 mg/kg),30 min后取小鼠胃组织和胃内容物进行RNA测序和代谢物检测,检测红茶干预不同状态下小鼠胃组织在转录组和代谢组层面的差异变化。结果发现,饱腹组红茶干预后,有907个基因表达上调,810个基因表达下调;空腹组红茶干预后,有748个基因表达上调,864个基因表达下调;对2组差异基因分别进行KEGG通路富集分析提示:生物节律、脂代谢、糖代谢等相关通路都存在显著差异,对生物节律通路相关核心基因进行检测发现饱腹组或空腹组红茶干预后生物钟基因Nr1d2、Per2、Per3表达显著上调,Arntl表达显著下调;胃内容物代谢:红茶干预后,饱腹组小鼠中的上调代谢物有1 355种,下调代谢物有1 393种,空腹组小鼠胃中的上调代谢物1 324种,下调代谢物1 378种,差异代谢物均被显著富集在氨基酸生物合成途径、嘌呤途径等。上述结果表明,红茶干预可能通过调控氨基酸生物合成途径影响小鼠的生物节律,进而调控小鼠胃节律运动、代谢等生物进程,最终起到胃助消化等功能的作用。
This study aimed to investigate the effects of black tea on gastric tissue and metabolism in mice and to analyze the effects on stomach biorhythm in satiated and fasted mice.6-week-old C57BL/6 satiated and fasted mice were administered normal saline and black tea extract (450 mg/kg), respectively.30 min later, the gastric tissues and stomach contents of the mice were taken for RNA sequencing and metabolite detection, and the differences in transcriptomic and metabolomic levels of the gastric tissues of mice under different conditions of black tea intervention were detected.Results indicated that 907 genes were up-regulated and 810 genes were down-regulated after black tea intervention in the satiated group.The expression of 748 genes were up-regulated and 864 genes were down-regulated in the fasted group after black tea intervention.Enrichment analysis of KEGG pathways for the two groups indicated that there were significant differences in biological rhythm, lipid metabolism, glucose metabolism, and other related pathways.The detection of core genes related to biological rhythm showed that the expression of circadian clock genes Nr1d2, Per2, and Per3 were significantly up-regulated and Arntl expression was significantly down-regulated after black tea intervention in the satiated group and fasting group.Metabolism results of stomach contents showed that after black tea intervention, there were 1 355 up-regulated metabolites and 1 393 down-regulated metabolites in the stomach of mice in the satiated group.In the fasted group, there were 1 324 up-regulated metabolites and 1 378 down-regulated metabolites.The metabolites showed significant enrichment of the amino acid biosynthesis and purine pathways.These findings show that black tea may affect the biological rhythm of mice by regulating amino acid biosynthesis, stomach rhythm, and metabolism.Finally, it may also play a role in stomach digestion.
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