甘油葡萄糖苷(2-O-α-D-glucopyranosyl-sn-glycerol,2-αGG)具有抗氧化活性和高保湿能力,在化妆品和制药领域具有广阔的应用前景。该文利用产蔗糖磷酸化酶的固定化细胞催化合成2-αGG,优化了κ-卡拉胶固定细胞的条件,通过0.02 g/mL的κ-卡拉胶与0.12 mg/mL的细胞混合制成固定化细胞颗粒,在甘油和蔗糖浓度均为1 mol/L的催化体系中,35 ℃时反应48 h后2-αGG的产量达到了39 g/L。此外还评价了2-αGG对HepG2细胞存活率的影响,结果表明,2-αGG浓度越高对HepG2细胞的抑制作用越明显。用2-αGG处理游离脂肪酸(free fatty acids,FFA)诱导的HepG2细胞后,与模型组相比,实验组细胞内脂滴减少,胆固醇、甘油三酯、低密度脂蛋白、高密度脂蛋白、丙氨酸氨基转移酶、天冬氨酸氨基转移酶指标下降,说明2-αGG可以改善由FFA诱导的非酒精性脂肪肝细胞模型的脂质沉积和肝损伤。
2-O-α-D-Glucopyranosyl-sn-glycerol (2-αGG) has high moisturizing and antioxidant properties, making it a promising ingredient in cosmetics and pharmaceuticals.In this study, the production of 2-αGG was achieved by immobilizing cells that produced sucrose phosphorylase.The optimal conditions for the immobilization of cells using κ-carrageenan were determined.Immobilized cell pellets were prepared by combining 0.02 g/mL κ-carrageenan with cells at a concentration of 0.12 mg/mL.At a glycerol-sucrose concentration of 1 mol/L, a 39 g/L yield of 2-αGG was obtained after a 48-hour reaction at 35 ℃.Moreover, the impact of 2-α GG on the survivability of HepG2 cells was studied.The higher the concentration, the greater the inhibitory effect observed on HepG2 cells.After treating HepG2 cells induced by free fatty acids (FFA) with 2-αGG, comparing the cells with the model group, the experimental group showed reduced intracellular lipid droplets, decreased levels of total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST).2-αGG has the potential to improve lipid deposition induced by FFA in non-alcoholic fatty liver cell.
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