研究报告

不同乳源乳铁蛋白在婴儿体外消化模型下的消化性差异研究

  • 樊逸菲 ,
  • 张丽娜 ,
  • 张玲艳 ,
  • 林洁 ,
  • 姜宇虹 ,
  • 周鹏
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  • 1(江南大学 食品学院,食品科学与资源挖掘全国重点实验室,江苏 无锡,214122)
    2(南京百斯杰生物工程有限公司,江苏 南京,211100)
第一作者: 硕士研究生(张丽娜副研究员为通信作者,E-mail:zhanglina@jiangnan.edu.cn)

收稿日期: 2025-01-24

  修回日期: 2025-03-20

  网络出版日期: 2025-08-01

基金资助

国家自然科学基金项目(32372246)

Difference of digestibility of lactoferrin from different milk sources in infant in vitro digestion model

  • FAN Yifei ,
  • ZHANG Lina ,
  • ZHANG Lingyan ,
  • LIN Jie ,
  • JIANG Yuhong ,
  • ZHOU Peng
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  • 1(State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)
    2(Nanjing Bestzyme Bio-engineering Co.Ltd., Nanjing 211100, China)

Received date: 2025-01-24

  Revised date: 2025-03-20

  Online published: 2025-08-01

摘要

该研究以市售牛乳铁、提取牛乳铁、人乳铁和驼乳铁为研究对象,通过测定其SDS-PAGE、多肽分子质量、水解度、游离氨基酸等一系列指标表征了不同乳源乳铁蛋白在婴儿体外消化模型下的消化性差异。4种乳铁蛋白的铁饱和度分别为16.02%、13.39%、7.49%、20.19%。结果表明,乳铁蛋白在胃内消化缓慢,进入肠阶段后被快速消化。其中提取牛乳铁蛋白比市售牛乳铁蛋白更易消化,骆驼乳铁蛋白在肠道内消化暂缓于其他物种乳铁,但其释放的游离氨基酸较多;而人乳铁蛋白在胃和肠都展现了较高的消化抗性,并且在肠初期消化有着明显缓于其他乳铁的趋势。该研究为婴儿营养产品配方优化、乳铁蛋白添加的提取和应用提供了可参考性。

本文引用格式

樊逸菲 , 张丽娜 , 张玲艳 , 林洁 , 姜宇虹 , 周鹏 . 不同乳源乳铁蛋白在婴儿体外消化模型下的消化性差异研究[J]. 食品与发酵工业, 2025 , 51(14) : 48 -57 . DOI: 10.13995/j.cnki.11-1802/ts.042239

Abstract

In this study, commercial lactoferrin, bovine lactoferrin, human lactoferrin, and camel lactoferrin were studied.The digestibility of lactoferrin from different milk sources was determined by SDS-PAGE, molecular weight of polypeptide, hydrolysis degree, and free amino acid.The iron saturation of the four lactoferrins was 16.03%, 13.55%, 7.49%, and 20.19%, respectively.Results showed that lactoferrin was digested slowly in the stomach and quickly after entering the intestinal stage.Bovine lactoferrin was easier to digest than commercially available bovine lactoferrin.Camel lactoferrin was digested in the gut more slowly than other species of lactoferrin, but it released more free amino acids.Human lactoferrin showed high digestive resistance in both the stomach and intestine and tended to delay the digestion of other lactoferrin in the early stage of intestinal digestion.This study provides a reference for infant nutrition product formulation optimization, extraction, and application of lactoferrin supplementation.

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