食品与发酵工业 >

2025 , Vol. 51 >Issue 14: 253 - 264

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042377

研究报告

魔芋凝胶质构特性对纳米硒功能特性的影响

  • 刘博文 ,
  • 孙洋 ,
  • 康宇 ,
  • 程凯旋 ,
  • 张驰 ,
  • 陈帅 ,
  • 商龙臣
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  • 1(湖北民族大学 生物与食品工程学院,湖北 恩施,445000)
    2(恩施土家族苗族自治州农业科学院,湖北 恩施,445000)
    3(武汉大学 公共卫生学院,湖北 武汉,430071)
第一作者: 硕士研究生(商龙臣讲师为通信作者,E-mail:2021021@hbmzu.edu.cn)

收稿日期: 2025-02-15

  修回日期: 2025-03-24

  网络出版日期: 2025-08-01

基金资助

湖北省自然科学基金计划青年项目(2023AFB162)湖北省教育厅科学研究计划资助项目(D20221902)湖北省自然科学基金联合基金项目(2024AFD079)

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Effects of texture properties on functional characteristics of nanosized selenium embedded in the konjac gel

  • LIU Bowen ,
  • SUN Yang ,
  • KANG Yu ,
  • CHENG Kaixuan ,
  • ZHANG Chi ,
  • CHEN Shuai ,
  • SHANG Longchen
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  • 1(College of Biological and Food Engineering, Hubei Minzu University, Enshi 445000, China)
    2(Enshi Tujia and Miao Autonomous Prefecture Academy of Agricultural Sciences, Hubei Minzu University, Enshi 445000, China)
    3(School of Public Health, Wuhan University, Wuhan 430071, China)

Received date: 2025-02-15

  Revised date: 2025-03-24

  Online published: 2025-08-01

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摘要

该研究采用不同质构特性的魔芋葡甘聚糖(konjac glucomannan, KGM)凝胶对纳米硒(selenium nanoparticles, SeNPs)进行封装,探讨了凝胶质构特性对SeNPs体外释放特性和生物活性的影响。研究结果显示,高硬度的KGM-SeNPs凝胶能够显著延缓SeNPs在胃肠消化过程中的释放,与低硬度的实验组相比,其在4 h内SeNPs释放速率降低了48.6%。自由基清除实验结果显示,高硬度KGM-SeNPs凝胶在12 h后表现出较强的生物活性,其对2种自由基清除率高于低硬度KGM-SeNPs凝胶。此外,该凝胶体系对大肠杆菌有显著的抑制效果,抑制率可达到66.2%,同时也对肿瘤细胞的增殖有一定的抑制率(36.4%)。该研究通过调控魔芋凝胶的质构特性以实现对SeNPs的可控释放,有效延长了其生物活性周期。不仅有可能提升SeNPs在生物体内的利用效率,同时也将降低其潜在的毒性风险。研究不仅为研发安全高效的硒营养补充剂提供了一定的理论基础,也有望为开拓SeNPs在功能性食品包装材料、生物医药敷料等生物活性材料方面的应用场景提供一定的思路借鉴。

关键词： 魔芋葡甘聚糖; 质构特性; 纳米硒; 缓释; 生物活性

本文引用格式

刘博文 , 孙洋 , 康宇 , 程凯旋 , 张驰 , 陈帅 , 商龙臣 . 魔芋凝胶质构特性对纳米硒功能特性的影响[J]. 食品与发酵工业, 2025 , 51(14) : 253 -264 . DOI: 10.13995/j.cnki.11-1802/ts.042377

Abstract

In this study, konjac glucomannan (KGM) gels with different textural properties were used to encapsulate selenium nanoparticles (SeNPs), and the effects of the textural properties of the gels on the in vitro release characteristics and bioactivities of SeNPs were investigated.Results showed that the high-hardness KGM-SeNPs gel significantly delayed the release of SeNPs during gastrointestinal digestion, and its release rate of SeNPs was reduced by 48.6% within 4 h compared with that of the experimental group with low hardness.The results of free radical scavenging experiments showed that the high-hardness KGM-SeNPs gels showed strong biological activity after 12 h, and their scavenging rates for the two free radicals were higher than those of the low-hardness KGM-SeNPs gels.In addition, the gel system had a significant inhibitory effect on Escherichia coli, with an inhibition rate of 66.2%, and inhibited the proliferation of tumor cells to a certain extent (36.4%).In the present study, by modulating the textural properties of konjac gels to control the release of SeNPs, the bioactivity cycle of SeNPs was effectively prolonged.This strategy not only enhanced the utilization efficiency of selenium nanoparticles in organisms but also reduced their potential toxicity risk.This study not only provided a certain theoretical basis for the development of safe, and efficient selenium nutritional supplements but also was expected to provide certain ideas for the development of application scenarios of nanosized selenium in functional food packaging materials, biomedical dressings, and other bioactive materials.

Key words： konjac glucomannan; textural characterization; selenium nanoparticles (SeNPs); slow release; bioactivity

参考文献

[1] 石文娟, 苗丽坤, 孙炜炜, 等. 魔芋葡甘聚糖基热可逆凝胶研究进展[J]. 中国食品学报, 2022, 22(5):422-433.
SHI W J, MIAO L K, SUN W W, et al. Research progress on the konjac glucomannan-based thermoreversible gels[J]. Journal of Chinese Institute of Food Science and Technology, 2022, 22(5):422-433.
[2] LIU Z J, REN X, CHENG Y Q, et al. Gelation mechanism of alkali induced heat-set konjac glucomannan gel[J]. Trends in Food Science & Technology, 2021, 116:244-254.
[3] XU C F, CHENG K X, KANG Y, et al. Deacetylated konjac glucomannan with a slower hydration rate delays rice digestion and weakens appetite response[J]. Molecules, 2024, 29(7):1681.
[4] ZHANG Y T, TONG C L, CHEN Y Y, et al. Advances in the construction and application of konjac glucomannan-based delivery systems[J]. International Journal of Biological Macromolecules, 2024, 262(Pt 1):129940.
[5] YANG X, LI A Q, LI D, et al. Applications of mixed polysaccharide-protein systems in fabricating multi-structures of binary food gels: A review[J]. Trends in Food Science & Technology, 2021, 109:197-210.
[6] 李佳芮, 李江华, 蔡超, 等. 基于海洋多糖水凝胶的组织工程材料研究应用进展[J]. 中国海洋药物, 2021, 40(1):79-89.
LI J R, LI J H, CAI C, et al. Advances in the research and application of tissue engineering scaffolds based on marine polysaccharide hydrogels[J]. Chinese Journal of Marine Drugs, 2021, 40(1):79-89.
[7] MANZOOR A, DAR A H, PANDEY V K, et al. Recent insights into polysaccharide-based hydrogels and their potential applications in food sector: A review[J]. International Journal of Biological Macromolecules, 2022, 213:987-1006.
[8] KALTSAS A. Oxidative stress and male infertility: The protective role of antioxidants[J]. Medicina, 2023, 59(10):1769.
[9] UMAPATHY S, PAN I, ISSAC P K, et al. Selenium nanoparticles as neuroprotective agents: Insights into molecular mechanisms for Parkinson’s disease treatment[J]. Molecular Neurobiology, 2025, 62(6):6655-6682.
[10] PETERS K M, GALINN S E, TSUJI P A. Selenium: Dietary Sources, Human Nutritional Requirements and Intake Across Populations[M]. Selenium. Cham: Springer International Publishing, 2016:295-305.
[11] ZHANG T, QI M, WU Q, et al. Recent research progress on the synthesis and biological effects of selenium nanoparticles[J]. Frontiers in Nutrition, 2023, 10:1183487.
[12] KALČEC N, PERANIĆ N, MAMIĆ I, et al. Selenium nanoparticles as potential drug-delivery systems for the treatment of Parkinson’s disease[J]. ACS Applied Nano Materials, 2023, 6(19):17581-17592.
[13] PURI A, MOHITE P, ANSARI Y, et al. Plant-derived selenium nanoparticles: Investigating unique morphologies, enhancing therapeutic uses, and leading the way in tailored medical treatments[J]. Materials Advances, 2024, 5(9):3602-3628.
[14] CHEN W W, CHENG H, XIA W S. Progress in the surface functionalization of selenium nanoparticles and their potential application in cancer therapy[J]. Antioxidants, 2022, 11(10):1965.
[15] XU X D, PAN Y X, LIU X Y, et al. Constructing selenium nanoparticles with enhanced storage stability and antioxidant activities via conformational transition of curdlan[J]. Foods, 2023, 12(3):563.
[16] SELMANI A, MLINARIĆ N M, FALSONE S F, et al. Simulated gastrointestinal fluids impact the stability of polymer-functionalized selenium nanoparticles: Physicochemical aspects[J]. International Journal of Nanomedicine, 2024, 19:13485-13505.
[17] GAD H A, ALSHUBAILY F A, ALSIENI M A, et al. Biosynthesis of nano-curcumin/nano-selenium composite and their potentialities as bactericides against fish-borne pathogens[J]. Green Processing and Synthesis, 2022, 11(1):1098-1107.
[18] 徐颖, 康佳敏, 魏宁果. 多糖-纳米硒复合物的合成方法、稳定性及生物活性研究进展[J]. 食品安全质量检测学报, 2023, 14(8):119-127.
XU Y, KANG J M, WEI N G. Research progress on the synthesis method, stability and biological activity of polysaccharide-nano-selenium complex[J]. Journal of Food Safety & Quality, 2023, 14(8):119-127.
[19] 吴玲莹. 负载纳米硒的温敏壳聚糖水凝胶黏膜下注射液的制备及表征[D]. 杭州:浙江理工大学, 2022.
WU L Y. Preparation and Characterization of Submucosal Injection of Temperature-Sensitive Chitosan Hydrogel Loaded with Nano-Selenium[D]. Hangzhou: Zhejiang Sci-Tech University, 2022.
[20] TRESPALACIOS P, PLA R. Synergistic action of transglutaminase and high pressure on chicken meat and egg gels in absence of phosphates[J]. Food Chemistry, 2007, 104(4):1718-1727.
[21] 李晶. 冷冻影响脱乙酰魔芋葡甘聚糖凝胶的机制与应用[D]. 武汉:华中农业大学, 2014.
LI J. Mechanism and application of freezing effect on deacetylkonjac glucomannan gel[D]. Wuhan:Huazhong Agricultural University, 2014.
[22] DAO L P, CHEN S Y, SUN X Y, et al. Construction and sustained release of konjac glucomannan/naringin composite gel spheres[J]. Frontiers in Nutrition, 2023, 9:1123494.
[23] KHIN M N, GOFF H D, NSOR-ATINDANA J, et al. Effect of texture and structure of polysaccharide hydrogels containing maltose on release and hydrolysis of maltose during digestion: in vitro study[J]. Food Hydrocolloids, 2021, 112:106326.
[24] 李爽, 刘小芳, 冷凯良, 等. 大西洋鳕鱼骨胶原蛋白肽的抗氧化活性及稳定性研究[J]. 食品与发酵工业, 2024, 50(16):50-56.
LI S, LIU X F, LENG K L, et al. Antioxidant activity and stability of collagen peptides from Atlantic cod (Gadus morhua) bone[J]. Food and Fermentation Industries, 2024, 50(16):50-56.
[25] 刘丽莉, 陈卉, 郭净芳, 等. 儿茶素-糖基化猪血红蛋白对肉糜品质的影响及抑菌作用[J]. 食品与发酵工业, 2024, 50(21):224-231.
LIU L L, CHEN H, GUO J F, et al. The effect of catechin glycosylated porcine hemoglobin on the quality of meat flour and its antibacterial effect[J]. Food and Fermentation Industries, 2024, 50(21):224-231.
[26] DENG L, XIA T T, CHENG W Q, et al. Injectable redox albumin-based hydrogel with in situ loaded dihydromyricetin[J]. Colloids and Surfaces B: Biointerfaces, 2022, 220:112871.
[27] GRADA A, OTERO-VINAS M, PRIETO-CASTRILLO F, et al. Research techniques made simple: Analysis of collective cell migration using the wound healing assay[J]. Journal of Investigative Dermatology, 2017, 137(2): e11-e16.
[28] 安玥琦. 鱼糜凝胶的交联结构对风味释放的影响与机制[D]. 武汉:华中农业大学, 2023.
AN Y Q. Effect and mechanism of cross-linking structure of surimi gel on flavor release[D]. Wuhan:Huazhong Agricultural University, 2023.
[29] GRENIER J, DUVAL H, BAROU F, et al. Mechanisms of pore formation in hydrogel scaffolds textured by freeze-drying[J]. Acta Biomaterialia, 2019, 94:195-203.
[30] YE S X, ZHU J S, SHAH B R, et al. Preparation and characterization of konjac glucomannan (KGM) and deacetylated KGM (Da-KGM) obtained by sonication[J]. Journal of the Science of Food and Agriculture, 2022, 102(10):4333-4344.
[31] SONG J Y, ZHOU J J, LI X, et al. Nano-selenium stablilized by Konjac Glucommannan and its biological activity in vitro[J]. LWT, 2022, 161:113289.
[32] 郭英凯, 赵燕禹, 王韬, 等. 纳米硒粉的制备及表征[J]. 天津工业大学学报, 2013, 32(6):45-48.
GUO Y K, ZHAO Y Y, WANG T, et al. Preparation and characterization of selenium nanoparticles[J]. Journal of Tianjin Polytechnic University, 2013, 32(6):45-48.
[33] 肖小年, 王凡, 周洁, 等. 甜菊糖苷改善根皮素溶解性及体外释放的研究[J]. 中国食品学报, 2021, 21(3):11-21.
XIAO X N, WANG F, ZHOU J, et al. Improving of solubilization and in vitro release enhancement of phloretin by steviol glycosides[J]. Journal of Chinese Institute of Food Science and Technology, 2021, 21(3):11-21.
[34] RAZZAQ H, SAIRA F, YAQUB A, et al. Interaction of gold nanoparticles with free radicals and their role in enhancing the scavenging activity of ascorbic acid[J]. Journal of Photochemistry and Photobiology B: Biology, 2016, 161:266-272.
[35] PU S, LI J, SUN L J, et al. An in vitro comparison of the antioxidant activities of chitosan and green synthesized gold nanoparticles[J]. Carbohydrate Polymers, 2019, 211:161-172.
[36] 王丽红, 杨辉, 苏文, 等. 植物乳杆菌LP21绿色合成纳米硒及对溶藻弧菌的抑菌活性[J]. 食品科学, 2022, 43(2):217-223.
WANG L H, YANG H, SU W, et al. Biosynthesis of nano-selenium by Lactobacillus plantarum LP21 and its antibacterial activity against Vibrio alginolyticus[J]. Food Science, 2022, 43(2):217-223.
[37] ADIBIAN F, GHADERI R S, SABOURI Z, et al. Green synthesis of selenium nanoparticles using Rosmarinus officinalis and investigated their antimicrobial activity[J]. BioMetals, 2022, 35(1):147-158.
[38] 王珍珍, 任奕梦, 罗之林, 等. 纳米硒的靶向递送及抗癌机制研究进展[J]. 沈阳药科大学学报, 2022, 39(1):105-113.
WANG Z Z, REN Y M, LUO Z L, et al. Research progress on targeted delivery of selenium nanoparticles and anti-cancer mechanism[J]. Journal of Shenyang Pharmaceutical University, 2022, 39(1):105-113.
[39] ADAM-DIMA E I, BALAS M, ANASTASESCU M, et al. Synthesis of homogeneous spherical selenium nanoparticles through a chemical method for cancer therapy applications[J]. Toxicology in Vitro, 2024, 95:105765.
[40] 韩晖琼, 王磊, 秦艳茹. Ovol2对食管鳞癌细胞增殖、迁移能力的影响和机制研究[J]. 海南医学, 2024, 35(15):2129-2134.
HAN H Q, WANG L, QIN Y R. Effect of Ovol2 on the proliferation and migration of esophageal squamous cell carcinoma cells and its related mechanism[J]. Hainan Medical Journal, 2024, 35(15):2129-2134.
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