食品与发酵工业 >

2025 , Vol. 51 >Issue 15: 80 - 87

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040920

研究报告

苯乳酸-肉桂醛联用对腐败希瓦氏菌的协同抑菌效果及作用机制

  • 杨晋一 ,
  • 耿珑瑶 ,
  • 杨乔越 ,
  • 黄云坡 ,
  • 张振
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  • (锦州医科大学 食品与健康学院,辽宁 锦州,121000)
第一作者:硕士研究生(张振副教授和黄云坡讲师为共同通信作者,E-mail:zhangzhen8849@163.com;59897537@qq.com)

收稿日期: 2024-09-01

  修回日期: 2024-11-23

  网络出版日期: 2025-08-22

基金资助

2023年辽宁省自然科学基金计划项目(博士启动)(2023-BS-200);锦州医科大学横向课题(H2023014);辽宁省教育厅2021年度基本科研项目(重点项目)(LJKZ0802)

收起

Synergistic bacteriostatic effect and mechanism of combination of phenyl lactic acid and cinnamaldehyde against Shewanella putrefaciens

  • YANG Jinyi ,
  • GENG Longyao ,
  • YANG Qiaoyue ,
  • HUANG Yunpo ,
  • ZHANG Zhen
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  • (College of Food and Health, Jinzhou Medical University, Jinzhou 121000, China)

Received date: 2024-09-01

  Revised date: 2024-11-23

  Online published: 2025-08-22

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摘要

随着食品工业对产品质量和安全性的要求不断提高,单一防腐剂的使用往往存在局限性,防腐剂联用因其降本增效等优势,具有重大的应用价值。苯乳酸作为当前高效防腐的天然抑菌物质,研究其复配联用效果对其生产应用具有良好意义。该研究采用苯乳酸和肉桂醛联用,以腐败希瓦氏菌为指示菌,通过最小抑菌浓度、复配液联合抑菌浓度指数(fractional inhibitory concentration index,FICI)测定,证明苯乳酸-肉桂醛的联合抑制效果;通过激光共聚焦显微镜、扫描电镜和傅里叶变换红外光谱仪等观察苯乳酸-肉桂醛联用后的抑菌作用机制;基于小牛胸腺DNA(calf thymus DNA,ct-DNA)模型,通过荧光光谱法探究苯乳酸-肉桂醛联用与ct-DNA的结合情况。研究结果表明,苯乳酸对指示菌的最小抑菌质量浓度为2 mg/mL,肉桂醛对指示菌的最小抑菌浓度为0.5 μL/mL,苯乳酸-肉桂醛复配联用的FICI值为0.75,表现出相加作用;激光共聚焦显微镜观察苯乳酸-肉桂醛联用后细胞膜的完整性破坏;扫描电子显微镜研究表明苯乳酸-肉桂醛联用使菌体表面凹陷且内容物流出,菌体断裂严重,有黏连现象,菌体细胞壁遭到破坏;傅里叶红外光谱研究表明苯乳酸-肉桂醛联用破坏了细胞壁和膜上的蛋白质和肽聚糖,破坏了磷脂双分子层;荧光光谱分析表明苯乳酸-肉桂醛联用能与ct-DNA发生沟槽结合。苯乳酸联用肉桂醛起到降本增效作用,同时作用机制表明苯乳酸-肉桂醛联用作用位点处包含细胞壁、膜。该研究基于细胞和分子层面探讨苯乳酸-肉桂醛复配的抑制效果与作用机制,为防腐保鲜剂的开发与应用提供一定的理论基础。

关键词： 腐败希瓦氏菌; 苯乳酸; 肉桂醛; 复配; 抑菌活性; 抑菌机制

本文引用格式

杨晋一 , 耿珑瑶 , 杨乔越 , 黄云坡 , 张振 . 苯乳酸-肉桂醛联用对腐败希瓦氏菌的协同抑菌效果及作用机制[J]. 食品与发酵工业, 2025 , 51(15) : 80 -87 . DOI: 10.13995/j.cnki.11-1802/ts.040920

Abstract

With the continuous improvement of product quality and safety requirements in the food industry, the use of single preservatives is often limited, and the combined use of preservatives has great application value because of its advantages such as cost reduction and efficiency increase.Phenyl lactic acid, as a natural bacteriostatic substance with high preservative efficiency, is of good significance for its production and application.In this study, phenyl lactic acid and cinnamaldehyde were used in combination, with Shewanella putrefaciens as the indicator bacteria.Through the determination of minimum inhibitory concentration and the fractional inhibitory concentration index (FICI) of the compound solution, the combined inhibitory effect of phenyl lactic acid and cinnamaldehyde was demonstrated.The antibacterial mechanism of the combination of phenyl lactic acid and cinnamaldehyde was observed by laser confocal microscopy, scanning electron microscopy, and Fourier transform infrared spectroscopy.Based on calf thymus DNA (ct-DNA) as a model, the binding situation of the combination of phenyl lactic acid and cinnamaldehyde with ct-DNA was explored through fluorescence spectrometry.The research results showed that the minimum inhibitory concentration of phenyl lactic acid for the indicator bacteria was 2 mg/mL and the minimum inhibitory concentration of cinnamaldehyde for the indicator bacteria was 0.5 μL/mL.The FICI value of the combined use of phenyl lactic acid and cinnamaldehyde was 0.75, showing an additive effect.Confocal laser scanning microscopy observation showed that the combination of phenyl lactic acid and cinnamaldehyde destroyed the integrity of the cell membrane.Scanning electron microscopy studies showed that the combination of phenyl lactic acid and cinnamaldehyde made the surface of the bacteria sunken and the contents flow out.The bacteria were severely broken, with an adhesion phenomenon, and the cell wall of the bacteria was damaged.Fourier transform infrared spectroscopy research showed that the combination of phenyl lactic acid and cinnamaldehyde destroyed proteins and peptidoglycans on the cell wall and membrane and destroyed the phospholipid bilayer.Fluorescence spectrum analysis showed that the combination of phenyl lactic acid and cinnamaldehyde could bind to ct-DNA through groove binding.The combination of phenyl lactic acid and cinnamaldehyde had the effect of reducing costs and increasing efficiency.At the same time, the mechanism of action showed that the action site of the combination of phenyl lactic acid and cinnamaldehyde included cell walls and membranes.This study explored the inhibitory effect and mechanism of action of the combination of phenyl lactic acid and cinnamaldehyde at the cellular and molecular levels, providing a certain theoretical basis for the development and application of antiseptic and fresh-keeping agents.

Key words： Shewanella putrefaciens; phenyl lactic acid; cinnamaldehyde; compounding; antibacterial activity; antibacterial mechanism

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