食品与发酵工业 >

2025 , Vol. 51 >Issue 15: 88 - 95

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041086

研究报告

ε-聚赖氨酸-壳聚糖复合保鲜液对荧光假单胞菌体外协同作用

  • 耿珑瑶 ,
  • 李丹丹 ,
  • 杨晋一 ,
  • 李杨心慧 ,
  • 吕晨鑫 ,
  • 黄云坡 ,
  • 张振
展开
  • (锦州医科大学 食品与健康学院,辽宁 锦州,121000)
第一作者:硕士研究生(张振副教授和黄云坡讲师为共同通信作者,E-mail:zhangzhen8849@163.com;huangyp@jzmu.edu.com)

收稿日期: 2024-09-18

  修回日期: 2024-11-28

  网络出版日期: 2025-08-22

基金资助

2023年辽宁省自然科学基金计划项目(博士启动)(2023-BS-200);锦州医科大学横向课题项目(H2023014);辽宁省教育厅2021年度基本科研项目(重点项目)(LJKZ0802)

收起

Synergistic effect of ε-polylysine chitosan composite preservation solution on Pseudomonas fluorescens in vitro

  • GENG Longyao ,
  • LI Dandan ,
  • YANG Jinyi ,
  • LI Yangxinhui ,
  • LYU Chenxin ,
  • HUANG Yunpo ,
  • ZHANG Zhen
Expand
  • (College of Food and Health, Jinzhou Medical University, Jinzhou 121000, China)

Received date: 2024-09-18

  Revised date: 2024-11-28

  Online published: 2025-08-22

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摘要

腐败微生物是影响水产预制加工制品保质期的主要因素之一。该研究探讨ε-聚赖氨酸-壳聚糖(ε-polylysine-chitosan,ε-PL-CS)复合保鲜液对荧光假单胞菌(Pseudomonas fluorescens)PF08的抑菌活性及其协同作用机制。利用时间-抑菌曲线、联合抑菌指数测定,分析ε-PL-CS复合保鲜液的协同效果;通过碱性磷酸酶(alkaline phosphatase,AKP)、ATP酶活力等指标的测定,并结合扫描电子显微镜和傅里叶变换红外光谱初步分析ε-PL-CS复合保鲜液对P.fluorescens PF08 的协同作用机制。结果表明,ε-PL对P.fluorescens PF08 最小抑菌浓度(minimum inhibitory concentration,MIC)为3 mg/mL;CS为5 mg/mL。ε-PL-CS复合保鲜液(1/4 ε-PL MIC+1/4 CS MIC)联用抑菌指数为0.5,表现为协同作用;ε-PL-CS复合保鲜液抑制AKP酶合成、胞内大分子出现泄露、菌体形态发生孔洞、断裂、黏连等现象;结合傅里叶变换红外光谱所呈现的代表脂质等分子成分官能团的改变,可以得出ε-PL-CS复合保鲜液能够改变壁、膜完整性,实现体外协同抑菌作用,为同类型复合保鲜剂的开发与研究提供理论基础。

关键词： ε-聚赖氨酸-壳聚糖; 荧光假单胞菌; 抑菌作用; 复合保鲜液; 协同作用

本文引用格式

耿珑瑶 , 李丹丹 , 杨晋一 , 李杨心慧 , 吕晨鑫 , 黄云坡 , 张振 . ε-聚赖氨酸-壳聚糖复合保鲜液对荧光假单胞菌体外协同作用[J]. 食品与发酵工业, 2025 , 51(15) : 88 -95 . DOI: 10.13995/j.cnki.11-1802/ts.041086

Abstract

Spoilage microorganisms are one of the main factors affecting the shelf life of prefabricated aquatic products.The purpose of this study was to explore the antibacterial activity and synergistic mechanism of ε-polylysine-chitosan (ε-PL-CS) composite preservation solution against Pseudomonas fluorescens PF08.A time inhibition curve was used and combined inhibition index measurement to analyze the synergistic effect of ε-PL-CS composite preservation solution.By measuring indicators such as AKP enzyme and ATPase activity and combining with scanning electron microscopy and Fourier transform infrared analysis, the synergistic mechanism of ε-PL-CS composite preservation solution on P.fluorescens PF08 was analyzed.Results indicated that the minimum inhibitory concentration (MIC) of ε-PL on P.fluorescens PF08 was 3 mg/mL and the MIC of CS on P.fluorescens PF08 was 5 mg/mL.The antibacterial index of the combination of ε-PL-CS composite preservation solution (1/4 ε-PLMIC+1/4 CSMIC) was 0.5, indicating a synergistic effect.The ε-PL-CS composite preservative solution inhibited AKP enzyme synthesis, caused leakage of intracellular macromolecules, and led to the formation of pores, fractures, and adhesions in bacterial morphology.Based on the changes in functional groups of molecular components such as lipids presented by Fourier transform infrared spectroscopy, it could be concluded that the ε-PL-CS composite preservative solution could alter the integrity of walls and membranes, achieve synergistic antibacterial effects in vitro, and provide a theoretical basis for the development and research of similar composite preservatives.

Key words： ε-polylysine-chitosan; Pseudomonas fluorescens; antibacterial effect; composite preservative solution; synergistic effect

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