Bacteria are extensively utilized in food and feed and the production of their additives, either being directly added or serving as the production strains of fermented products such as enzyme preparations and amino acids.The issue of bacterial antibiotic resistance has emerged as a focal point of attention both domestically and internationally, and it constitutes a core aspect of the safety evaluation of food and feed cultures.The advent of next-generation sequencing technology has provided a potent tool for the gene safety evaluation of bacteria.The latest research progress in the safety evaluation of antibiotic resistance genes in food and feed bacteria was reviewed from the aspects of antibiotics, resistance mechanisms and antibiotic resistance gene analysis strategies in the context of whole genome sequencing, aiming to provide a scientific basis and reference for the establishment of a safety evaluation system for industrial microbial strains in China.
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