该文旨在探究细菌素paracin wx3对胡萝卜果胶杆菌胡萝卜亚种(Pectobacterium carotovorum subsp.carotovorum,Pcc)CBJ3引起的辣椒软腐病的控制效果及其对辣椒贮藏品质的影响,为辣椒软腐病防控及维持辣椒良好品质提供新思路。结果表明细菌素paracin wx3对辣椒软腐病具有良好的控制作用:在体外,细菌素paracin wx3对Pcc的最小抑菌浓度(minimal inhibit concentration,MIC)为16 μg/mL;在辣椒体内,细菌素paracin wx3浓度越高,辣椒软腐病发病率越低、病斑直径越小,16 MIC paracin wx3可以完全抑制辣椒发病。绿色荧光蛋白标记Pcc CBJ3的菌落计数结果表明,细菌素paracin wx3处理可显著降低辣椒上的病原菌数量。扫描电镜结果表明,paracin wx3可以通过在细胞膜上形成孔杀死果胶杆菌,从而减少辣椒表皮病原菌的分布量,进而维持辣椒组织完好性,减轻软腐症状。同时,细菌素paracin wx3处理可以保持较好的果实品质,控制辣椒在贮藏期间的失重减少,可维持较好的硬度、可溶性固形物含量和果实色泽,还可以减少维生素C的流失,防止丙二醛含量的升高。顶空-气相色谱-离子迁移谱结果表明,细菌素paracin wx3处理可以减少辣椒5-甲基-3-庚酮、丙烯腈、二甲胺等挥发性物质的产生,减少释放恶臭气味。该研究可为辣椒软腐病生物防控提供技术参考。
The article aims to explore the control effect of bacteriocin paracin wx3 on pepper soft rot induced by Pectobacterium carotovorum subsp.carotovorum (Pcc) CBJ3 and its impact on pepper storage quality, providing new ideas for the control of pepper soft rot and the maintaining of good pepper quality.Results showed that bacteriocin paracin wx3 had a good control effect on pepper soft rot.In vitro, the minimal inhibition concentration (MIC) of paracin wx3 against Pcc was 16 μg/mL.Inside of pepper, the higher the concentration of paracin wx3, the lower the incidence of soft rot and the smaller the diameter of lesions, and 16 MIC paracin wx3 could completely inhibit pepper disease.The colony counting result of green fluorescent protein labelled Pcc CBJ3 showed that bacteriocin paracin wx3 treatment could significantly reduce the number of pathogens on pepper.The result of scanning electron microscopy showed that paracin wx3 could kill Pcc by forming holes in the cell membrane, thereby reducing the distribution of pathogen on pepper surface, then maintaining the integrity of pepper tissue and reducing soft rot symptoms.At the same time, bacteriocin paracin wx3 treatment could maintain good fruit quality, control weight loss during storage, maintain good fruit hardness, soluble solid content, and good fruit color, and also could reduce the decrease of vitamin C contents and prevent the increase of MDA.The result of headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) showed that bacteriocin paracin wx3 treatment could reduce the production of volatile substances, such as 5-methyl-3-heptenone, acrylonitrile, and dimethylamine, and reduce the release of offensive odors.This study can provide technical references for the biological control of pepper soft rot.
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