Modern soy sauce evolved and developed from soybean paste, the latter originated from “minced meat”, fermented meat sauce, indicating that meat can be used as a raw material for fermented soy sauce, but soy sauce produced with pork as (part of) raw material has not yet been reported by modern literature.To enrich the variety of soy sauces in China and to investigate the effect of pork on soy sauce flavor, this study developed pork soy sauce by using pork instead of partial soybeans in accordance with the high-salt dilution process.The impact of pork on soy sauce flavor and its physicochemical indexes were analyzed by using quantitative sensory evaluation, conventional physicochemical analyses, and gas chromatography-mass spectrometry (GC-MS)/gas chromatography-olfactometry (GC-O).Results of the sensory evaluation showed that the addition of pork increased the umami taste by 13.79%, and the alcoholic, fruity, malty, and caramel-like aromas by 35.04%, 25.46%, 23.55%, and 13.46% compared with those of the control soy sauce.Analyses of proximate indexes showed that formaldehyde nitrogen content of the pork-added soy sauce increased by 2.50%-8.64%, and DPPH radical scavenging capacity and reducing power increased by 7.44%-22.60% and 25.85%-40.18% compared with those of the control soy sauce.From the above results, it could be concluded that adding an appropriate amount of pork not only significantly improved the flavor of soy sauce, but also enhanced the antioxidant activities of soy sauce.GC-MS/GC-O analysis showed that the content of compounds with fatty aroma and compounds with malty, alcoholic, and fruity aromas unique to pork soy sauce were increased by 39.73%, 32.07%, and 52.95% compared with those of the control soy sauce.The pork-specific 1-penten-3-ol, heptanal, E,E-2,4-decadienal, and nonanal conferred the fatty aroma of pork sauce.In this study, a pork soy sauce with a unique flavor was developed by using pork instead of a certain proportion of soybeans, which could not only enrich the variety of soy sauce and provide consumers with more kinds of soy sauce choices but also inherit and carry forward the traditional dietary culture of China.
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