猪肉酱油开发及其风味物质分析

  • 侯美含 ,
  • 张展开 ,
  • 张智宏 ,
  • 马海乐 ,
  • 高献礼
展开
  • (江苏大学 食品与生物工程学院,江苏 镇江,212013)
第一作者:本科生(高献礼教授为通信作者,E-mail:gaoxianli@ujs.edu.cn)

收稿日期: 2024-12-15

  修回日期: 2025-02-19

  网络出版日期: 2025-11-03

基金资助

国家自然科学基金项目(32372254);国家级大学生创新创业训练计划项目(202410299076Z)

Development of pork soy sauce and identification of its flavor compounds

  • HOU Meihan ,
  • ZHANG Zhankai ,
  • ZHANG Zhihong ,
  • MA Haile ,
  • GAO Xianli
Expand
  • (School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China)

Received date: 2024-12-15

  Revised date: 2025-02-19

  Online published: 2025-11-03

摘要

现代酱油是从豆酱演变和发展而来,而豆酱又起源于“醢”,即发酵肉酱,说明肉类可以作为发酵酱油的原料,但目前以猪肉为(部分)原料生产的酱油在现代文献中尚未见报道。为丰富我国酱油品种和探明猪肉对酱油风味的影响,该研究利用猪肉代替部分大豆,按照高盐稀态工艺开发了猪肉酱油,并利用定量感官评价、常规理化分析和气相色谱-质谱/气相色谱-嗅闻(gas chromatography-mass spectrometry/gas chromatography-olfactometry, GC-MS/GC-O)等方法分析了猪肉对酱油风味和理化指标的影响。感官评价结果表明,添加适量的猪肉相比对照酱油的鲜味提高13.79%,醇香、果香、麦芽香和焦糖香相比对照酱油提高35.04%、25.46%、23.55%和13.46%。常规理化指标分析表明,添加猪肉的样品酱油相比对照酱油的氨基酸态氮含量提高2.50%~8.64%,DPPH自由基清除能力和还原力分别提高7.44%~22.60%和25.85%~40.18%。由以上结果可知,添加适量猪肉不但显著改善酱油风味,而且可以提高酱油抗氧化活性。GC-MS/GC-O分析表明,猪肉酱油具有的脂香香气的化合物及麦芽香、醇香、果香香气的化合物含量相比对照酱油分别提高了39.73%、32.07%、52.95%。猪肉特有的1-戊烯-3-醇、庚醛、E,E-2,4-癸二烯醛、壬醛等物质赋予了猪肉酱油脂香。该研究利用猪肉代替一定比例大豆开发了一种具有独特风味的猪肉酱油,不但可以丰富酱油品种,为消费者提供更多种类的酱油选择,而且可以继承和弘扬我国传统饮食文化。

本文引用格式

侯美含 , 张展开 , 张智宏 , 马海乐 , 高献礼 . 猪肉酱油开发及其风味物质分析[J]. 食品与发酵工业, 2025 , 51(19) : 366 -374 . DOI: 10.13995/j.cnki.11-1802/ts.041866

Abstract

Modern soy sauce evolved and developed from soybean paste, the latter originated from “minced meat”, fermented meat sauce, indicating that meat can be used as a raw material for fermented soy sauce, but soy sauce produced with pork as (part of) raw material has not yet been reported by modern literature.To enrich the variety of soy sauces in China and to investigate the effect of pork on soy sauce flavor, this study developed pork soy sauce by using pork instead of partial soybeans in accordance with the high-salt dilution process.The impact of pork on soy sauce flavor and its physicochemical indexes were analyzed by using quantitative sensory evaluation, conventional physicochemical analyses, and gas chromatography-mass spectrometry (GC-MS)/gas chromatography-olfactometry (GC-O).Results of the sensory evaluation showed that the addition of pork increased the umami taste by 13.79%, and the alcoholic, fruity, malty, and caramel-like aromas by 35.04%, 25.46%, 23.55%, and 13.46% compared with those of the control soy sauce.Analyses of proximate indexes showed that formaldehyde nitrogen content of the pork-added soy sauce increased by 2.50%-8.64%, and DPPH radical scavenging capacity and reducing power increased by 7.44%-22.60% and 25.85%-40.18% compared with those of the control soy sauce.From the above results, it could be concluded that adding an appropriate amount of pork not only significantly improved the flavor of soy sauce, but also enhanced the antioxidant activities of soy sauce.GC-MS/GC-O analysis showed that the content of compounds with fatty aroma and compounds with malty, alcoholic, and fruity aromas unique to pork soy sauce were increased by 39.73%, 32.07%, and 52.95% compared with those of the control soy sauce.The pork-specific 1-penten-3-ol, heptanal, E,E-2,4-decadienal, and nonanal conferred the fatty aroma of pork sauce.In this study, a pork soy sauce with a unique flavor was developed by using pork instead of a certain proportion of soybeans, which could not only enrich the variety of soy sauce and provide consumers with more kinds of soy sauce choices but also inherit and carry forward the traditional dietary culture of China.

参考文献

[1] 张智宏, 陈嘉琳, 黄鑫, 等.酱油的历史及原酿造酱油发展趋势[J].现代食品科技, 2023, 39(12):302-309.
ZHANG Z H, CHEN J L, HUANG X, et al.The history of soy sauce and developmental trends in naturally-fermented soy sauce[J].Modern Food Science and Technology, 2023, 39(12):302-309.
[2] 冯云子, 周婷, 吴伟宇, 等.酱油风味与功能性成分研究进展[J].食品科学技术学报, 2021, 39(4):14-28.
FENG Y Z, ZHOU T, WU W Y, et al.Research progress on flavor and functional components of soy sauce[J].Journal of Food Science and Technology, 2021, 39(4):14-28.
[3] GAO X L, ZHAO X, HU F, et al.The latest advances on soy sauce research in the past decade:Emphasis on the advances in China[J].Food Research International, 2023, 173:113407.
[4] 于林, 陈义伦, 吴澎, 等.我国史籍记载的酱及酱油历史起源研究[J].山东农业大学学报 (社会科学版), 2015, 17(1):14-17;22;113.
YU L, CHEN Y L, WU P, et al.The origin of sauce and soy sauce in historical records of China[J].Journal of Shandong Agricultural University (Social Science Edition), 2015, 17(1):14-17;22;113.
[5] 陈静如, 胡锋, 符姜燕, 等.利用富硒双孢菇粉提升酱油有机硒含量和改善酱油风味[J].现代食品科技, 2023, 39(5):217-224.
CHEN J R, HU F, FU J Y, et al.Enhancing the organic selenium content in soy sauce and improving the soy sauce flavor using selenium-enriched Agaricus bisporus powder[J].Modern Food Science and Technology, 2023, 39(5):217-224.
[6] 夏小乐, 吴剑荣, 陈坚.传统发酵食品产业技术转型升级战略研究[J].中国工程科学, 2021, 23(2):129-137.
XIA X L, WU J R, CHEN J.Technology upgrading strategy for traditional fermented food industry in China[J].Strategic Study of CAE, 2021, 23(2):129-137.
[7] 高献礼, 马海乐, 任晓锋, 等.一种发酵法生产乌鸡酱油的方法:中国, CN201510435153.3[P].2018-04-17.
GAO X L, MA H L, REN X F, et al.A fermentation method for producing black chicken soy sauce:China, CN201510435153.3[P].2018-04-17.
[8] 李武笋, 汤晓艳.猪肉及其副产物的生物活性成分及营养功能研究进展[J].农产品质量与安全, 2024(2):5-10;30.
LI W S, TANG X Y.Research progress on bioactive components and nutritional functions of pork and its by-products[J].Quality and Safety of Agro-Products, 2024(2):5-10;30.
[9] GAO X L, CUI C, REN J Y, et al.Changes in the chemical composition of traditional Chinese-type soy sauce at different stages of manufacture and its relation to taste[J].International Journal of Food Science & Technology, 2011, 46(2):243-249.
[10] BRAND-WILLIAMS W, CUVELIER M E, BERSET C.Use of a free radical method to evaluate antioxidant activity[J].LWT - Food Science and Technology, 1995, 28(1):25-30.
[11] LEE J H, HWANG C E, SON K S, et al.Comparisons of nutritional constituents in soybeans during solid state fermentation times and screening for their glucosidase enzymes and antioxidant properties[J].Food Chemistry, 2019, 272:362-371.
[12] ZHENG L, ZHAO Y J, DONG H Z, et al.Structure-activity relationship of antioxidant dipeptides:Dominant role of Tyr, Trp, Cys and Met residues[J].Journal of Functional Foods, 2016, 21:485-496.
[13] TCHABO W, MA Y K, KWAW E, et al.Effects of ultrasound, high pressure, and manosonication processes on phenolic profile and antioxidant properties of a sulfur dioxide-free mulberry (Morus nigra) wine[J].Food and Bioprocess Technology, 2017, 10(7):1210-1223.
[14] GAO X L, SHAN P, FENG T, et al.Enhancing selenium and key flavor compounds contents in soy sauce using selenium-enriched soybean[J].Journal of Food Composition and Analysis, 2022, 106:104299.
[15] FENG Y Z, CAI Y, SU G W, et al.Evaluation of aroma differences between high-salt liquid-state fermentation and low-salt solid-state fermentation soy sauces from China[J].Food Chemistry, 2014, 145:126-134.
[16] GAO X L, ZHANG J K, LIU E M, et al.Enhancing the taste of raw soy sauce using low intensity ultrasound treatment during moromi fermentation[J].Food Chemistry, 2019, 298:124928.
[17] 崔艺燕, 马现永.猪肉风味研究进展[J].肉类研究, 2017, 31(6):55-60.
CUI Y Y, MA X Y.Recent advances in the research on pork flavor compounds[J].Meat Research, 2017, 31(6):55-60.
[18] 尹添柱, 商振达, 董冰.藏猪肉风味特性及其形成途径研究进展[J].中国畜牧杂志, 2023, 59(12):49-55.
YIN T Z, SHANG Z D, DONG B.Research progress on flavor characteristics and formation pathways of Tibetan pork[J].Chinese Journal of Animal Science, 2023, 59(12):49-55.
[19] FENG Y Z, CHEN Z Y, LIU N, et al.Changes in fatty acid composition and lipid profile during koji fermentation and their relationships with soy sauce flavour[J].Food Chemistry, 2014, 158:438-444.
[20] SU P, TIAN Y F, SU G W, et al.Differences in taste and material basis of soybean and defatted soybean fermented soy sauces and influence factors[J].Journal of Food Composition and Analysis, 2024, 136:106768.
[21] LIU Z P, ZHANG X L, DUAN X R, et al.Effect of fermentation conditions on the formation of ammonium salt in soy sauce[J].LWT, 2022, 153:112492.
[22] ZHOU T, FENG Y Z, THOMAS-DANGUIN T, et al.Enhancement of saltiness perception by odorants selected from Chinese soy sauce:A gas chromatography/olfactometry-associated taste study[J].Food Chemistry, 2021, 335:127664.
[23] GAO X L, LIU E M, ZHANG J K, et al.Effects of sonication during moromi fermentation on antioxidant activities of compounds in raw soy sauce[J].LWT, 2019, 116:108605.
[24] KIM M J, KWAK H S, KIM S S.Effects of salinity on bacterial communities, Maillard reactions, isoflavone composition, antioxidation and antiproliferation in Korean fermented soybean paste (doenjang)[J].Food Chemistry, 2018, 245:402-409.
[25] 张雅琼. 酱油类黑精的结构鉴定及其抗氧化活性初步研究[D].镇江:江苏大学,2023.
ZHANG Y Q.Structural identification and preliminary study on antioxidant activity of soy sauce[D].Zhenjiang:Jiangsu University, 2023.
[26] XU X Y, MA C M, YANG Y, et al.Effects of phytic acid from soybean meal on Maillard reaction and antioxidant properties of products[J].Food Chemistry, 2025, 463:141257.
[27] CHEN X C, MENG F B, TIAN H L, et al.Dynamic changes in metabolomics and flavoromics during industrial scale fermentation of Chinese traditional soy sauce[J].Food Bioscience, 2024, 59:103940.
[28] YAO Y S, HUANG M G, WANG X M, et al.Characteristic volatile compounds contributed to aroma of braised pork and their precursor sources[J].Food Chemistry, 2024, 459:140335.
[29] 刘浩悦, 李聪, 王颖, 等.国内黑猪肉风味研究进展[J].现代食品科技, 2023, 39(9):337-352.
LIU H Y, LI C, WANG Y, et al.Research progress on the flavor of black pork in China[J].Modern Food Science and Technology, 2023, 39(9):337-352.
文章导航

/