食品与发酵工业 >

2025 , Vol. 51 >Issue 19: 422 - 428

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042203

植物油中苯并(a)芘检测技术的研究进展

  • 栾亚林 ,
  • 谢刚 ,
  • 罗凤莲 ,
  • 郭玉垚 ,
  • 张颖
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  • 1(上海理工大学 健康科学与工程学院,上海,200093)
    2(国家粮食和物资储备局科学研究院,北京,100037)
第一作者:硕士研究生(谢刚研究员和罗凤莲副研究员为共同通信作者,E-mail:xg@ags.ac.cn;lfl@ags.ac.cn)

收稿日期: 2025-01-20

  修回日期: 2025-03-20

  网络出版日期: 2025-11-03

基金资助

中央级公益性科研院所基本科研业务费专项资金项目(ZX2442)

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Research progress on the detection technology of benzo (a) pyrene in vegetable oils

  • LUAN Yalin ,
  • XIE Gang ,
  • LUO Fenglian ,
  • GUO Yuyao ,
  • ZHANG Ying
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  • 1(School of Healthy Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China)
    2(Academy of National Food and Strategic Reserves Administration, Beijing 100037, China)

Received date: 2025-01-20

  Revised date: 2025-03-20

  Online published: 2025-11-03

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摘要

苯并(a)芘[benzo (a) pyrene,BaP],作为多环芳烃(polycyclic aromatic hydrocarbons,PAHs)家族的一员,因其显著的致癌与致突变特性,对人类健康及生态环境构成了不容忽视的威胁,这一问题已在全球范围内引起了广泛重视。在食品领域,尤其是植物油的生产过程中,由于高温加工步骤如焙炒等,使得BaP成为植物油中潜在的高风险污染物。该文概述了植物油中BaP检测技术的最新进展,涵盖免疫学方法ELISA、表面增强拉曼光谱技术、HPLC、GC-MS以及HPLC-MS/MS等。这些研究不仅可以推动检测技术的创新与进步,而且对于确保植物油产品的安全性具有重要的意义。

关键词： 植物油; 苯并(a)芘; 检测方法; 研究现状; 食品安全

本文引用格式

栾亚林 , 谢刚 , 罗凤莲 , 郭玉垚 , 张颖 . 植物油中苯并(a)芘检测技术的研究进展[J]. 食品与发酵工业, 2025 , 51(19) : 422 -428 . DOI: 10.13995/j.cnki.11-1802/ts.042203

Abstract

Benzo (a) pyrene (BaP), as one of the polycyclic aromatic hydrocarbons (PAHs), exhibits strong indirect carcinogenicity and mutagenicity, posing a serious threat to human health and the environment.This problem has aroused wide attention in the world.In the field of food, especially in the production of vegetable oils, BaP has become a potentially high-risk pollutant due to high temperature roasting.The latest progress in the detection of BaP in vegetable oils was reviewed, covering immunoassay, surface-enhanced Raman spectroscopy, HPLC, GC-MS, and HPLC-MS/MS.These studies can not only promote the innovation and progress of detection technology, but also have important significance for ensuring the safety of vegetable oil products.

Key words： vegetable oils; benzo (a) pyrene; detection methods; research status; food safety

参考文献

[1] BUKOWSKA B, MOKRA K, MICHAŁOWICZ J.Benzo[a] pyrene-environmental occurrence, human exposure, and mechanisms of toxicity[J].International Journal of Molecular Sciences, 2022, 23(11):6348.
[2] MENG H, LI G, WEI W, et al.Epigenome-wide DNA methylation signature of benzo[a] pyrene exposure and their mediation roles in benzo[a] pyrene-associated lung cancer development[J].Journal of Hazardous Materials, 2021, 416:125839.
[3] ALI MALLAH M, LI C X, ALI MALLAH M, et al.Polycyclic aromatic hydrocarbon and its effects on human health:An overeview[J].Chemosphere, 2022, 296:133948.
[4] SINGH L, AGARWAL T, SIMAL-GANDARA J.Summarizing minimization of polycyclic aromatic hydrocarbons in thermally processed foods by different strategies[J].Food Control, 2023, 146:109514.
[5] 陈颖华. 金浩茶油涉嫌致癌 百姓何“食” 为安?[J].上海标准化, 2010, 29(10):19-20.
CHEN Y H.Jin Hao tea oil is suspected of causing cancer.Why should people ‘eat’ for safety?[J].Shanghai Standardization, 2010, 29(10):19-20.
[6] YANG J, CHEN R D, WANG C, et al.A widely targeted metabolite modificomics strategy for modified metabolites identification in tomato[J].Journal of Integrative Plant Biology, 2024, 66(4):810-823.
[7] 范素芳, 马俊美, 俞婧, 等.固相萃取—高效液相色谱法测定植物油中苯并(a)芘[J].河北省科学院学报, 2017, 34(2):47-51.
FAN S F, MA J M, YU J, et al.Determination of the benzo (a) pyrene in vegetable oil by high performance liquid chromatography based on solid phase extraction[J].Journal of Hebei Academy of Sciences, 2017, 34(2):47-51.
[8] 张艳燕, 温建荣, 钟坚海, 等.高效液相色谱法快速测定食用植物油中苯并(a)芘的研究[J].食品与发酵科技, 2019, 55(6):127-129.
ZHANG Y Y, WEN J R, ZHONG J H, et al.Fast determination of benzo(a) pyrene in edible vegetable oil by high performance liquid chromatography[J].Food and Fermentation Sciences & Technology, 2019, 55(6):127-129.
[9] 曾云军, 汪泽生.全自动固相萃取净化-高效液相色谱法测定食用植物油中苯并(a)芘含量[J].中国油脂, 2023, 48(4):149-152.
ZENG Y J, WANG Z S.Determination of benzo(a) pyrene in edible vegetable oil by automated solid phase extraction-high performance liquid chromatography[J].China Oils and Fats, 2023, 48(4):149-152.
[10] 李文廷, 刘玲, 夏加恩, 等.分子印迹固相萃取-高效液相色谱法的优化及在测定植物油中苯并(a)芘含量的应用[J].粮油食品科技, 2023, 31(1):150-157.
LI W T, LIU L, XIA J E, et al.Optimization of molecularly imprinted solid phase extraction-high performance liquid chromatography and its application in determination of benzo(a) pyrene in vegetable oil[J].Science & Technology of Cereals, Oils & Foods, 2023, 31(1):150-157.
[11] 许文雅, 刘瀛, 周长民, 等.全自动固相萃取-高效液相色谱法测定植物油中苯并(a)芘[J].食品与发酵科技, 2023, 59(6):150-154.
XU W Y, LIU Y, ZHOU C M, et al.Determination of benzo (a) pyrene in vegetable oils by automatic solid phase extraction-high performance liquid chromatography[J].Food and Fermentation Sciences & Technology, 2023, 59(6):150-154.
[12] 徐艺溶, 利东玲, 杨昆, 等.液液萃取-高效液相色谱法快速测定植物油中的苯并(a)芘含量[J].实验室检测, 2024, 2(11):7-11.
XU Y R, LI D L, YANG K, et al.Rapid determination of benzo[a] pyrene in vegetable oil by liquid-liquid extraction-high performance liquid chromatography[J].Laboratory Testing, 2024, 2(11):7-11.
[13] HUERTAS-PÉREZ J F, BASLÉ Q, DUBOIS M, et al.Multi-residue pesticides determination in complex food matrices by gas chromatography tandem mass spectrometry[J].Food Chemistry, 2024, 436:137687.
[14] WEI Y X, HUANG C Q, CHEN L, et al.Determination of chlordimeform and its metabolite residue in milk by gas chromatography-tandem mass spectrometry[J].Food Research International, 2024, 192:114754.
[15] PURCARO G, MORET S, CONTE L S.Rapid validated method for the analysis of benzo[a] pyrene in vegetable oils by using solid-phase microextraction-gas chromatography-mass spectrometry[J].Journal of Chromatography A, 2007, 1176(1-2):231-235.
[16] WANG J H, GUO C.Ultrasonication extraction and gel permeation chromatography clean-up for the determination of polycyclic aromatic hydrocarbons in edible oil by an isotope dilution gas chromatography-mass spectrometry[J].Journal of Chromatography A, 2010, 1217(28):4732-4737.
[17] AMZAD HOSSAIN M, SALEHUDDIN S M.Polycyclic aromatic hydrocarbons (PAHs) in edible oils by gas chromatography coupled with mass spectroscopy[J].Arabian Journal of Chemistry, 2012, 5(3):391-396.
[18] 陈冰, 肖维毅, 尧珍玉 等.磁性多壁碳纳米管固相萃取-气相色谱质谱联用快速测定食用油中的苯并(a)芘[J].中国测试, 2015(11):44-49.
CHEN B, XIAO W Y, YAO Z Y, et al.Magnetic multi-walled carbon nanotubes as a solid phase extraction adsorbent for the determination of benzo (a) pyrene in cooking oil by gas chromatography-mass spectrometry[J].China Measurement & Testing Technology, 2015(11):44-49.
[19] 杨卫花, 寸宇智.气相色谱-串联质谱法测定食用植物油中的苯并(a)芘[J].食品安全质量检测学报, 2017, 8(5):1852-1856.
YANG W H, CUN Y Z.Determination of benzo (a) pyrene in edible vegetable oils by gas chromatography-tandem mass spectrometry[J].Journal of Food Safety & Quality, 2017, 8(5):1852-1856.
[20] 王国庆, 王宗义, 马子夏, 等.气相色谱串联质谱法检测食用植物油中的4种欧盟限量多环芳烃[J].现代食品科技, 2018, 34(3):242-247.
WANG G Q, WANG Z Y, MA Z X, et al.Determination of 4 types of polycyclic aromatic hydrocarbons limited by EU regulation in edible oils by gas chromatography tandem mass spectrometry[J].Modern Food Science and Technology, 2018, 34(3):242-247.
[21] 王磊, 樊蕊.凝胶色谱-气质联用测定花生油中苯并芘残留[J].食品工业, 2019, 40(5):322-324.
WANG L, FAN R.Determination of benzopyrene in peanut oil by gel permeation chromatography-gas chromatography/mass spectrometry[J].The Food Industry, 2019, 40(5):322-324.
[22] 宗万里, 王妍, 周宏霞.固相萃取-气相色谱-三重四级杆串联质谱法测定花生油中苯并(a)芘含量[J].黄河科技学院学报, 2020, 22(11):79-82.
ZONG W L, WANG Y, ZHOU H X.Determination of benzo (α) pyrene in peanut oil by SPE-GC-MS/MS[J].Journal of Huanghe S&T University, 2020, 22(11):79-82.
[23] NEMATI M, TUZEN M, ALI FARAZAJDEH M, et al.Development of dispersive solid-liquid extraction method based on organic polymers followed by deep eutectic solvents elution;application in extraction of some pesticides from milk samples prior to their determination by HPLC-MS/MS[J].Analytica Chimica Acta, 2022, 1199:339570.
[24] TANG Y Y, YANG G X, LIU X X, et al.Rapid sample enrichment, novel derivatization, and high sensitivity for determination of 3-chloropropane-1, 2-diol in soy sauce via high-performance liquid chromatography-tandem mass spectrometry[J].Journal of Agricultural and Food Chemistry, 2023, 71(41):15388-15397.
[25] IZZO L, NARVÁEZ A, CASTALDO L, et al.Multiclass and multi-residue screening of mycotoxins, pharmacologically active substances, and pesticides in infant milk formulas through ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry analysis[J].Journal of Dairy Science, 2022, 105(4):2948-2962.
[26] 刘玉兰, 张小涛, 赵欢欢.液相色谱-串联质谱法检测食用油脂中苯并(a)芘[J].农业机械, 2012(30):45-48.
LIU Y L, ZHANG X T, ZHAO H H.Detection of benzo (a) pyrene in edible oils by liquid chromatography - tandem mass spectrometry[J].Farm Machinery, 2012(30):45-48.
[27] 王浩, 杨红梅, 郭启雷, 等.高效液相色谱-串联质谱法同时测定植物油中苯并芘与黄曲霉毒素B1, B2, G1, G2[J].分析测试学报, 2014, 33(8):911-916.
WANG H, YANG H M, GUO Q L, et al.Simultaneous determination of benzo(a) pyrene and aflatoxins(B1, B2, G1, G2) in vegetable oil by high performance liquid chromatography-tandem mass spectrometry[J].Journal of Instrumental Analysis, 2014, 33(8):911-916.
[28] 张晓艺, 张秀尧, 蔡欣欣, 等.超高效液相色谱-大气压化学电离-三重四极杆质谱联用法快速测定食品中苯并[a] 芘[J].色谱, 2017, 35(6):608-612.
ZHANG X Y, ZHANG X Y, CAI X X, et al.Rapid determination of benzo[a] pyrene in foods by ultra performance liquid chromatography-triple quadrupole mass spectrometry with atmospheric pressure chemical ionization[J].Chinese Journal of Chromatography, 2017, 35(6):608-612.
[29] 廖浩. 超高效液相色谱-三重四极杆质谱法快速测定煎炸过程用油中的苯并[a] 芘[J].食品工业科技, 2018, 39(19):248-251;257.
LIAO H.Rapid determination of benzo[a] pyrene in deep frying oil by ultra performance liquid chromatography-triple quadrupole mass spectrometry[J].Science and Technology of Food Industry, 2018, 39(19):248-251;257.
[30] 王玉娇, 冯潇慧, 冯胜楠, 等.液相色谱-串联质谱法定量检测油脂中16种多环芳烃[J].食品安全质量检测学报, 2024, 15(16):20-30.
WANG Y J, FENG X H, FENG S N, et al.Quantitative determination of 16 kinds of polycyclic aromatic hydrocarbons in fats and oils by liquid chromatography-tandem mass spectrometry[J].Journal of Food Safety & Quality, 2024, 15(16):20-30.
[31] KETNEY O, SANTINI A, OANCEA S.Recent aflatoxin survey data in milk and milk products:A review[J].International Journal of Dairy Technology, 2017, 70(3):320-331.
[32] PSCHENITZA M, HACKENBERG R, NIESSNER R, et al.Analysis of benzo[a] pyrene in vegetable oils using molecularly imprinted solid phase extraction (MISPE) coupled with enzyme-linked immunosorbent assay (ELISA)[J].Sensors, 2014, 14(6):9720-9737.
[33] 刘波, 王宇, 戚平, 等.食用油中致癌物苯并(a)芘的快速免疫检测胶体金层析试纸条研制[J].中国油脂, 2016, 41(7):68-72.
LIU B, WANG Y, QI P, et al.Development of colloidal gold immunochromatographic strip for rapid detection of carcinogens benzo(a) pyrene in edible oil[J].China Oils and Fats, 2016, 41(7):68-72.
[34] 郝梦. 胡麻油苯并(a)芘污染水平的ELISA和HPLC法检测评价[D].呼和浩特:内蒙古农业大学, 2018.
HAO M.Determination and evaluation on benzo pyrenes contaminantin flaxseed oil by ELISA and HPLC[D].Hohhot:Inner Mongolia Agricultural University, 2018.
[35] 张健. 酶联免疫吸附法在食品检验中的应用进展[J].食品安全导刊, 2020(9):159.
ZHANG J.Application progress of enzyme-linked immunosorbent assay in food inspection[J].China Food Safety Magazine, 2020(9):159.
[36] NIE X, DONG K, TIAN Y, et al.A green analysis detection of formaldehyde in grooming products by surface enhanced Raman spectroscopy[J].Materials Today Sustainability, 2023, 22:100303.
[37] LIU C, XU D, DONG X, et al.A review:Research progress of SERS-based sensors for agricultural applications[J].Trends in Food Science & Technology, 2022, 128:90-101.
[38] 王珊, 李忠, 潘亮, 等.表面增强拉曼光谱(SERS)快速检测食用油中的苯并(a)芘[J].现代食品科技, 2017, 33(9):271-275.
WANG S, LI Z, PAN L, et al.Study on rapid quantification of benzo[a] pyrene in edible oil by surface enhanced Raman spectroscopy[J].Modern Food Science and Technology, 2017, 33(9):271-275.
[39] 钱立. 表面增强拉曼光谱法用于食用油中苯并(a)芘的快速检测[D].扬州:扬州大学, 2021.
QIAN L.Surface enhanced Raman spectroscopy for rapid detection of benzo(a)pyrene in cooking oil[D].Yangzhou:Yangzhou University, 2021.
[40] 李沁怡, 郭小玉, 杨海峰.表面增强拉曼光谱检测食用油中的苯并(a)芘[J].上海师范大学学报(自然科学版中英文), 2024, 53(5):612-617.
LI Q Y, GUO X Y, YANG H F.Detection of benzo (a) pyrene in edible oil by surface enhanced Raman spectroscopy[J].Journal of Shanghai Normal University (Natural Sciences), 2024, 53(5):612-617.
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