研究报告

茶多酚对氧化引发剂诱导的休闲豆干品质劣化的保护作用研究

  • 黄嘉琪 ,
  • 刁欣蕊 ,
  • 向佳豪 ,
  • 王榆颉 ,
  • 周衡平 ,
  • 陈浩 ,
  • 赵良忠 ,
  • 李金成 ,
  • 黄展锐
展开
  • (邵阳学院 食品与化学工程学院,豆制品加工与安全控制湖南省重点实验室,湘味特色食品绿色加工与装备湖南省工程研究中心,湖南 邵阳,422000)
第一作者:硕士研究生(李金成教授和黄展锐副教授为共同通信作者,E-mail:1209748954@qq.com;zhanrui_huang@163.com)

收稿日期: 2025-01-22

  修回日期: 2025-03-12

  网络出版日期: 2025-12-15

基金资助

邵阳学院研究生科研创新项目(CX2023SY020);湖南省重点实验室建设项目(2019TP1028);湖南省自然科学基金区域联合基金项目(2024JJ7485);湖南省教育厅科学研究项目(23A0542);邵阳市科技计划项目(2024PT4043)

Study on protective effect of tea polyphenols on quality deterioration of leisure dried tofu induced by oxidative initiators

  • HUANG Jiaqi ,
  • DIAO Xinrui ,
  • XIANG Jiahao ,
  • WANG Yujie ,
  • ZHOU Hengping ,
  • CHEN Hao ,
  • ZHAO Liangzhong ,
  • LI Jincheng ,
  • HUANG Zhanrui
Expand
  • (College of Food and Chemical Engineering, Shaoyang University, Hunan Provincial Key Laboratory of Soybean Products Processing and Safety Control, Hunan Engineering Research Center of Green Processing and Equipment of Hunan-style Food, Shaoyang 422000, China)

Received date: 2025-01-22

  Revised date: 2025-03-12

  Online published: 2025-12-15

摘要

该研究采用茶多酚和氧化引发剂处理的卤汁制备休闲豆干,从营养成分、特征氧化指标、质构特性、分子间作用力和微观结构等角度分析引发剂对休闲豆干品质的影响及茶多酚的保护作用。结果显示,引发剂组豆干贮藏90 d后蛋白质和脂肪含量分别降低至15.05 g/100 g、11.06 g/100 g,而水分含量显著增高至56.23 g/100 g;与空白组相比,引发剂组豆干特征氧化指标和b*值随贮藏时间延长显著增高,质构特性、L*值和二硫键作用力逐渐降低,蛋白质凝胶结构出现孔隙增大、结构断裂现象,表明光酶引发剂易诱导豆干蛋白质氧化而造成其品质劣化;0.2%(质量分数)茶多酚可以通过维持蛋白质凝胶网络结构,在15~60 d能有效防止引发剂诱导豆干营养成分、色差和质构特性等氧化作用变化,但在75~90 d保护作用较弱或不明显。该研究结果可为拓宽茶多酚应用和休闲豆干质量安全控制提供理论依据。

本文引用格式

黄嘉琪 , 刁欣蕊 , 向佳豪 , 王榆颉 , 周衡平 , 陈浩 , 赵良忠 , 李金成 , 黄展锐 . 茶多酚对氧化引发剂诱导的休闲豆干品质劣化的保护作用研究[J]. 食品与发酵工业, 2025 , 51(22) : 288 -295 . DOI: 10.13995/j.cnki.11-1802/ts.042226

Abstract

In this study, leisure dried tofu (LD-tofu) was prepared with the marinade treated with tea polyphenols (TP) and oxidation initiators, and the effects of initiators on the quality of LD-tofu and the protective effects of TP were analyzed from the perspectives of nutritional composition, characteristic oxidation indicators, texture characteristics, intermolecular forces, and microstructure.Results showed that the protein and fat contents of LD-tofu in oxidation initiator group decreased to 15.05 g/100 g, 11.06 g/100 g, respectively, during storage for 90 days, while the water content increased significantly to 56.23 g/100 g.Compared with the control group, the characteristic oxidation indexes and b* value of LD-tofu in the oxidation initiator group increased significantly with the storage time, and the texture characteristics, L* value, and disulfide bond interaction force gradually decreased.The protein gel structure showed pore enlargement and structural fracture, indicating that the photoenzyme initiators easily induced the protein oxidation of LD-tofu, resulting in its quality degradation.TP (0.2%) could effectively prevent oxidation initiator induced changes in nutritional composition, color difference, texture characteristics, and other oxidative effects of LD-tofu by maintaining the protein gel network structure during 15-60 days, but the protective effect was weak or not noticeable during 75-90 days.The research results can provide a theoretical basis for expanding the application of TP and quality safety control of LD-tofu.

参考文献

[1] 吴国庆, 黄展锐, 郑奕柔, 等.不同卤汁循环次数下卤豆干及卤汁品质特性变化规律研究[J].食品与发酵工业, 2024, 50(16):228-234;241.
WU G Q, HUANG Z R, ZHENG Y R, et al.Study on change rule of quality characteristics of leisure-dried tofu and marinade under different marinade recycling cycles[J].Food and Fermentation Industries, 2024, 50(16):228-234;241.
[2] HUANG Z R, ZHOU H P, JIANG Q H, et al.Study on the quality change and deterioration mechanism of leisure dried tofu under different storage temperature conditions[J].LWT, 2022, 172:114257.
[3] LI Y B, LIN L D, HUANG Z R, et al.An assessment of the occupational environment contributing to the microbial contamination and diversity of leisure dried tofu[J].LWT, 2024, 209:116790.
[4] 卜宇芳, 李文强, 谢灵来, 等.休闲豆腐干贮藏过程中品质变化研究[J].食品与机械, 2016, 32(2):115-118.
BU Y F, LI W Q, XIE L L, et al.Research on quality change of leisure dried Tofu during storage[J].Food & Machinery, 2016, 32(2):115-118.
[5] 伍涛, 彭希林, 赵良忠, 等.湘派豆干及卤汁在卤制过程中的变化规律[J].食品与发酵工业, 2021, 47(19):146-154.
WU T, PENG X L, ZHAO L Z, et al.Variation law of Xiangpai dried tofu and brine during marinating process[J].Food and Fermentation Industries, 2021, 47(19):146-154.
[6] 卜宇芳, 赵良忠, 尹乐斌, 等.卤豆干生产过程微生物检测及安全控制[J].食品科学, 2014, 35(5):107-110.
BU Y F, ZHAO L Z, YIN L B, et al.Microbial detection and safety control during production of stewed dried tofu[J].Food Science, 2014, 35(5):107-110.
[7] 陈浩, 杨莹, 赵良忠, 等.湘派卤汁循环使用安全监测及预警模型的构建[J].食品与发酵工业, 2020, 46(21):181-187.
CHEN H, YANG Y, ZHAO L Z, et al.The safety monitoring and early-warning model construction for Xiangpai brine recycling[J].Food and Fermentation Industries, 2020, 46(21):181-187.
[8] 蒋琼华, 赵良忠, 李化强, 等.休闲豆制品贮藏期的品质变化及腐败机理研究[J].食品与机械, 2015, 31(1):116-119;211.
JIANG Q H, ZHAO L Z, LI H Q, et al.Research on quality changes and spoilage mechanism of leisure soybean food during storage[J].Food & Machinery, 2015, 31(1):116-119;211.
[9] HUANG Y Q, ZHENG Y R, HUANG Z R, et al.Evaluating the effect of different recycling frequencies on marinade quality, characteristics, and volatile constituents[J].Food Science and Biotechnology, 2025, 34(4):893-904.
[10] TAZI S, PLANTEVIN F, DI FALCO C, et al.Effects of light, temperature and water activity on the kinetics of lipoxidation in almond-based products[J].Food Chemistry, 2009, 115(3):958-964.
[11] RAN M, HE L P, LI C Q, et al.Quality changes and shelf-life prediction of cooked cured ham stored at different temperatures[J].Journal of Food Protection, 2021, 84(7):1252-1264.
[12] AL-DALALI S, LI C, XU B C.Effect of frozen storage on the lipid oxidation, protein oxidation, and flavor profile of marinated raw beef meat[J].Food Chemistry, 2022, 376:131881.
[13] 夏璜金, 陈玉涟, 谢恒, 等.休闲豆干37 ℃下贮藏的品质变化规律[J].农产品加工, 2022(6):52-58.
XIA H J, CHEN Y L, XIE H, et al.The quality change rule of leisure bean dry stored at 37 ℃[J].Farm Products Processing, 2022(6):52-58.
[14] 周衡平, 黄展锐, 赵良忠, 等.不同氧化条件对卤汁和卤豆干营养成分的影响[J].农产品加工, 2024(9):9-13.
ZHOU H P, HUANG Z R, ZHAO L Z, et al.Effects of different oxidation conditions on nutritional components of marinate and marinated dried tofu[J].Farm Products Processing, 2024(9):9-13.
[15] 李欣, 郭咪咪, 范文广, 等.食用植物油抗氧化的研究进展[J].粮油食品科技, 2024, 32(3):109-116.
LI X, GUO M M, FAN W G, et al.Research progress on the antioxidation of edible vegetable oils[J].Science and Technology of Cereals, Oils and Foods, 2024, 32(3):109-116.
[16] 陈露, 尹礼国, 张超, 等.茶多酚在发酵食品中的应用研究进展[J].食品与发酵工业, 2023, 49(2):339-346.
CHEN L, YIN L G, ZHANG C, et al.Review on the application of tea polyphenols in fermented foods[J].Food and Fermentation Industries, 2023, 49(2):339-346.
[17] 李秀娟, 黄莉, 丁波, 等.茶多酚对猪油在不同热加工条件的抗氧化作用[J].食品研究与开发, 2018, 39(8):220-224.
LI X J, HUANG L, DING B, et al.Antioxidant effect of tea polyphenol for lard with various heating treatment[J].Food Research and Development, 2018, 39(8):220-224.
[18] OBEME-NMOM J I, ABIOYE R O, REYES FLORES S S, et al.Regulation of redox enzymes by nutraceuticals:A review of the roles of antioxidant polyphenols and peptides[J].Food & Function, 2024, 15(22):10956-10980.
[19] 侯天宇, 谢礼文, 梁永霞, 等.不同精炼程度山茶油油炸过程中品质变化的研究[J].中国粮油学报, 2024, 39(9):107-114.
HOU T Y, XIE L W, LIANG Y X, et al.Study on quality changes of camellia oil during frying with different refining degrees[J].Journal of the Chinese Cereals and Oils Association, 2024, 39(9):107-114.
[20] 毛佳怡. 加卤技术对卤豆干风味品质影响研究[D].贵阳:贵州大学, 2020.
MAO J Y.Effects of different marinating methods on the quality and flavor profiles of marinated dried bean curd[D].Guiyang:Guizhou University, 2020.
[21] RUDRAPAL M, RAKSHIT G, SINGH R P, et al.Dietary polyphenols:Review on chemistry/sources, bioavailability/ metabolism, antioxidant effects, and their role in disease management[J].Antioxidants, 2024, 13(4):429.
[22] JIANG Y F, SU M K, YU T, et al.Quantitative determination of peroxide value of edible oil by algorithm-assisted liquid interfacial surface enhanced Raman spectroscopy[J].Food Chemistry, 2021, 344:128709.
[23] WANG J, LI B, CHEN X, et al.Interactions between tea polyphenols and soy protein:Mechanisms and effects on protein oxidation-induced aggregation[J].Food Hydrocolloids, 2020, 105:105793.
[24] JIANG J, XIONG Y L.Natural antioxidants as food and feed additives to promote health benefits and quality of meat products:A review[J].Meat Science, 2016, 120:107-117.
[25] ZUO F, CHEN Z J, SHI X D, et al.Yield and textural properties of tofu as affected by soymilk coagulation prepared by a high-temperature pressure cooking process[J].Food Chemistry, 2016, 213:561-566.
[26] 徐立荣. 食用油贮藏过程自动氧化变化规律研究[D].杨凌:西北农林科技大学, 2017.
XU L R.Autoxidative change and its law of edible oils during storage[D].Yangling:Northwest Agriculture & Forestry University, 2017.
[27] 陈曦, 汪娟, 孙为正.亚油酸氧化诱导大豆分离蛋白氧化对其结构的影响[J].食品与发酵工业, 2017, 43(9):87-91.
CHEN X, WANG J, SUN W Z.Effect of protein oxidation induced by linoleic acid oxidation on the structure of soy protein isolate[J].Food and Fermentation Industries, 2017, 43(9):87-91.
[28] 吴伟, 林亲录, 华欲飞.脂质氢过氧化物氧化修饰对大豆蛋白去折叠和复折叠性质的影响[J].中国粮油学报, 2011, 26(7):17-21;52.
WU W, LIN Q L, HUA Y F.Effects of oxidative modification by lipid hydroperoxide on unfolding and refolding properties of soy protein[J].Journal of the Chinese Cereals and Oils Association, 2011, 26(7):17-21;52.
[29] HUANG Z R, SUN J, ZHAO L Z, et al.Analysis of the gel properties, microstructural characteristics, and intermolecular forces of soybean protein isolate gel induced by transglutaminase[J].Food Science & Nutrition, 2022, 10(3):772-783.
[30] HOFFMANN M A M, VAN MIL P J J M.Heat-induced aggregation of β-lactoglobulin:Role of the free thiol group and disulfide bonds[J].Journal of Agricultural and Food Chemistry, 1997, 45(8):2942-2948.
[31] CAROCHO M, MORALES P, FERREIRA I C F R.Antioxidants:Reviewing the chemistry, food applications, legislation and role as preservatives[J].Trends in Food Science & Technology, 2018, 71:107-120.
文章导航

/