该文以中蜂鸭脚木蜜与意蜂鸭脚木蜜为研究对象,利用顶空固相微萃取-气质联用技术分析其特征性挥发成分,在此基础上,扩大中蜂蜂蜜与意蜂蜂蜜的植物源,验证2个蜂种蜂蜜的特征挥发性成分。结果显示,在中蜂与意蜂鸭脚木蜜中共鉴定110种化合物,其中47种为共有成分(42.73%),以萜烯类和醇类为主。中蜂鸭脚木蜜中三烯醇、芳樟醇氧化物、酯类及酚类含量高于意蜂鸭脚木蜜,且含35种特有成分,而意蜂鸭脚木蜜则含28种特有成分。经扩大样品验证月桂酸甲酯与3-蒈烯在中蜂蜂蜜中稳定检出,而辛烷、辛酸甲酯和癸酸甲酯则特异分布于意蜂蜂蜜,上述化合物是基于挥发性成分的中、意蜂蜂蜜鉴别的关键物质,该研究结果为中蜂蜂蜜和意蜂蜂蜜的鉴别提供了新的参考。
This study used Apis cerana honey and Apis mellifera honey as research subjects and analyzed their volatile components using headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS).Then, this study expanded the nectar sources of both honeys to verify the characteristic volatile components.A total of 110 compounds were identified, with 47(42.73%) common to both types, primarily terpenes and alcohols.A.cerana honey contained more trienol, linalool oxide, esters, and phenols, with 35 unique components, while A.mellifera honey had 28 specific components.Further analysis showed methyl laurate and 3-carene were stable in A.cerana honey, while octane, methyl octanoate, methyl decanoate were unique to A.mellifera honey.These volatile differences provide key chemical markers for identifying honey species.The study will provide a novel approach to the foundation for honey authentication.
[1] FAUSTINO C, PINHEIRO L.Analytical rheology of honey:A state-of-the-art review[J].Foods, 2021, 10(8):1709.
[2] ESCUREDO O, SEIJO M C.Honey:Chemical composition, stability and authenticity[J].Foods, 2019, 8(11):577.
[3] 杨明华, 谭霞, 宋文菲, 等.源自中华蜜蜂和西方蜜蜂的澳洲坚果蜜挥发性成分比较分析[J].云南农业大学学报(自然科学), 2022, 37(2):269-277.
YANG M H, TAN X, SONG W F, et al.Comparative analysis of volatile compounds in macadamia honey from chinese and western honeybees[J].Journal of Yunnan Agricultural University (Natural Science), 2022, 37(2):269-277.
[4] MAHMOUD M A A, K1L1Ç-BÜYÜKKURT Ö, ABOUL FOTOUH M M, et al.Aroma active compounds of honey:Analysis with GC-MS, GC-O, and molecular sensory techniques[J].Journal of Food Composition and Analysis, 2024, 134:106545.
[5] JERKOVIĆI, KUŚP M.Terpenes in honey:Occurrence, origin and their role as chemical biomarkers[J].RSC Advances, 2014, 4(60):31710-31728.
[6] ŠTEFÁNIKOVÁ J, MARTIŠOVÁ P, ŠNIRC M, et al.Screening of the honey aroma as a potential essence for the aromachology[J].Applied Sciences, 2021, 11(17):8177.
[7] JUAN-BORRÁS M, PERICHE A, DOMENECH E, et al.Correlation between methyl anthranilate level and percentage of pollen in Spanish citrus honey[J].International Journal of Food Science & Technology, 2015, 50(7):1690-1696.
[8] ŠPÁNIK I, PAŽITNÁ A, ŠIŠKA P, et al.The determination of botanical origin of honeys based on enantiomer distribution of chiral volatile organic compounds[J].Food Chemistry, 2014, 158:497-503.
[9] CASTRO-VÁZQUEZ L, LEON-RUIZ V, ALAñON M E, et al.Floral origin markers for authenticating Lavandin honey (Lavandula angustifolia x latifolia).Discrimination from Lavender honey (Lavandula latifolia)[J].Food Control, 2014, 37:362-370.
[10] SEISONEN S, KIVIMA E, VENE K.Characterisation of the aroma profiles of different honeys and corresponding flowers using solid-phase microextraction and gas chromatography-mass spectrometry/olfactometry[J].Food Chemistry, 2015, 169:34-40.
[11] ZHANG Y Z, SI J J, LI S S, et al.Chemical analyses and antimicrobial activity of nine kinds of unifloral Chinese honeys compared to manuka honey (12+ and 20+)[J].Molecules, 2021, 26(9):2778.
[12] KASIOTIS K M, BAIRA E, IOSIFIDOU S, et al.Fingerprinting chemical markers in the Mediterranean orange blossom honey:UHPLC-HRMS metabolomics study integrating melissopalynological analysis, GC-MS and HPLC-PDA-ESI/MS[J].Molecules, 2023, 28(9):3967.
[13] JERKOVIĆI, PRDUN S, MARIJANOVIĆZ, et al.Traceability of Satsuma mandarin (Citrus unshiu Marc.) honey through nectar/honey-sac/honey pathways of the headspace, volatiles, and semi-volatiles:Chemical markers[J].Molecules, 2016, 21(10):1302.
[14] LIU X T, SUN J, JI P R, et al.Hydroxy fatty acids as novel markers for authenticity identification of the honey entomological origin based on the GC-MS method[J].Journal of Agricultural and Food Chemistry, 2023, 71(18):7163-7173.
[15] 何亮亮. 枸杞蜜的理化性质及其抗氧化活性谱效关系研究[D].西安:西北大学, 2019.
HE L L.Physicochemical properties and antioxidant spectrum-effect relationship of medlar honey[D].Xi’an:Northwest University, 2019.
[16] 祝敏. 西北五种特色单花种蜂蜜花源特征性成分及其对酒精性胃损伤的保护作用研究[D].西安:西北大学, 2021.
ZHU M.Characteristic components of five special monofloral honeys from northwest China and the gastroprotective effect against ethanol-induced gastric injury[D].Xi’an:Northwest University, 2021.
[17] NAKIB R, SHANTAL RODRÍGUEZ-FLORES M, ESCUREDO O, et al.Retama sphaerocarpa, Atractylis serratuloides and Eruca sativa honeys from Algeria:Pollen dominance and volatile profiling (HS-SPME/GC-MS)[J].Microchemical Journal, 2022, 174:107088.
[18] THRASYVOULOU A, TANANAKI C, GORAS G, et al.Legislation of honey criteria and standards[J].Journal of Apicultural Research, 2018, 57(1):88-96.
[19] CARTER C, SHAFIR S, YEHONATAN L, et al.A novel role for proline in plant floral nectars[J].Naturwissenschaften, 2006, 93(2):72-79.
[20] 问亚琴, 张金振, 金玥, 等.基于多酚类物质和脯氨酸的多元统计分析鉴别不同植物源单花蜜[C].二十一世纪第二届全国蜂业科技与蜂产业发展大会论文集.北京: 2016.
WEN Y Q, ZHANG J Z, JIN Y, et al.Characterization of Chinese monofloral honeys from different botanical origins with multivariate analysis of proline andphenolic profile as markers[C].Proceedings of the 2nd National Apicultural Science & Technology And Industry Development Conference In 21 st Century Bee World.Beijing, 2016.
[21] 章晋武. 工蜂酿蜜生物学特性研究[D].南昌:江西农业大学, 2024.
ZHANG J W.Study on biological characteristics of worker bee processing nectar[D].Nanchang:Jiangxi Agricultural University, 2024.
[22] 王欣然. 中蜂蜜和意蜂蜜的识别及肠道抗炎活性研究[D].北京:中国农业科学院, 2022.
WANG X R.Study of identification and intestinal anti-inflammatory activity of A.cerana honey and A.mellifera honey[D].Beijing:Chinese Academy of Agricultural Sciences, 2022.
[23] 王桃红, 崔宗岩, 张少博, 等.中蜂与意蜂荔枝蜜挥发性成分的测定与对比分析[J].食品研究与开发, 2020, 41(7):159-166.
WANG T H, CUI Z Y, ZHANG S F, et al.Determination and comparative analysis of volatile compounds in lychee honeys produced by Apis cerana cerana and Apis mellifera ligustica[J].Food Research And Development, 2020, 41(7):159-166.
[24] PATTAMAYUTANON P, ANGELI S, THAKEOW P, et al.Biomedical activity and related volatile compounds of thai honeys from 3 different honeybee species[J].Journal of Food Science, 2015, 80(10):M2228-M2240.
[25] 王银, 刘彩玲, 苟心怡, 等.秦岭中蜂蜂蜜酚类提取物的鉴定及其抑菌活性研究[J].食品与发酵工业, 2022, 48(7):154-159.
WANG Y, LIU C L, GOU X Y, et al.Phenolic compounds of Qinling Apis cerana honey and its antimicrobial activity against foodborne pathogens[J].Food and Fermentation Industries, 2022, 48(7):154-159.
