食品与发酵工业 >

2025 , Vol. 51 >Issue 24: 172 - 177

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042590

研究报告

遵义地区鲊广椒细菌多样性分析及乳酸菌分离鉴定

  • 杨心怡 ,
  • 黄怡 ,
  • 杨莹 ,
  • 李卫华 ,
  • 王婷 ,
  • 郭壮 ,
  • 王玉荣
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  • 1(湖北文理学院 湖北省食品配料工程技术研究中心,湖北 襄阳,441053)
    2(湖北文理学院 乳酸菌生物技术与工程襄阳市重点实验室,湖北 襄阳,441053)
    3(襄阳市公共检验检测中心,湖北 襄阳,441001)
第一作者:本科生(王玉荣副教授为通信作者,E-mail:wang_HFu6456@163.com)

收稿日期: 2025-03-03

  修回日期: 2025-03-19

  网络出版日期: 2026-01-12

基金资助

国家自然科学基金青年科学基金项目(32402047);湖北省高等学校优秀中青年科技创新团队计划项目(T2023022)

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Bacterial diversity analysis, isolation and identification of lactic acid bacteria of Zha-chili in Zunyi region

  • YANG Xinyi ,
  • HUANG Yi ,
  • YANG Ying ,
  • LI Weihua ,
  • WANG Ting ,
  • GUO Zhuang ,
  • WANG Yurong
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  • 1(Hubei Provincial Engineering and Technology Research Center for Food Ingredients, Hubei University of Arts and Science, Xiangyang 441053, China)
    2(Xiangyang Lactic Acid Bacteria Biotechnology and Engineering Key Laboratory, Hubei University of Arts and Science, Xiangyang 441053, China)
    3(Xiangyang Public Inspection and Testing Center, Xiangyang 441001, China)

Received date: 2025-03-03

  Revised date: 2025-03-19

  Online published: 2026-01-12

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摘要

该研究以采集自遵义地区的16 份鲊广椒样品为研究对象,通过PacBio单分子实时(single molecule real-time, SMRT)测序技术对细菌多样性进行解析,同时采用纯培养技术对乳酸菌进行分离鉴定。属水平分析结果显示,优势细菌属以促生乳杆菌属(Levilactobacillus)、乳酸杆菌属(Lactobacillus)和伴生乳杆菌属(Companilactobacillus)为主,平均相对含量分别为33.57%、32.79%和13.30%;种水平结果显示,优势细菌种以耐酸乳杆菌(Lactobacillus acetotolerans)为主,平均相对含量为30.34%;分类操作单元(operational taxonomic units,OTU)分析结果显示,核心细菌类群为隶属于Levilactobacillus的OTU3240和OTU2736,两者累计相对含量为8.56%;纯培养结果显示,从鲊广椒中共分离出59株乳酸菌,分别被鉴定为8个属下的15个种,其中消化伴生乳杆菌(Companilactobacillus alimentarius)、植物乳植杆菌(Lactiplantibacillus plantarum)和戊糖乳植物杆菌(Lactiplantibacillus pentosus)为优势分离株,分别占总分离株的23.73%、22.03%和11.86%。由此可见,遵义地区鲊广椒中蕴含了丰富的乳酸菌资源,并且具有较高的物种多样性。

关键词： 鲊广椒; PacBio SMRT测序技术; 纯培养技术; 细菌多样性; 乳酸菌

本文引用格式

杨心怡 , 黄怡 , 杨莹 , 李卫华 , 王婷 , 郭壮 , 王玉荣 . 遵义地区鲊广椒细菌多样性分析及乳酸菌分离鉴定[J]. 食品与发酵工业, 2025 , 51(24) : 172 -177 . DOI: 10.13995/j.cnki.11-1802/ts.042590

Abstract

Sixteen samples of Zha-chili were collected from Zunyi region as the research object.The bacterial diversity was analyzed using PacBio single molecule real time (SMRT) sequencing technology, and pure culture technology was used to isolate and identify lactic acid bacteria.The results of genus level analysis showed that the dominant bacterial genera were mainly Levilactobacillus, Lactobacillus, and Companiobacillus, with average relative contents of 33.57%, 32.79%, and 13.30%, respectively.The results at the species level showed that the dominant bacterial species were mainly Lactobacillus acetollerans, with an average relative content of 30.34%.The analysis results of operational taxonomic units (OTUs) showed that the core bacterial groups were OTU3240 and OTU2736 belonging to the genus Levilactobacillus, with a cumulative relative content of 8.56%.The pure culture results showed that a total of 59 strains of lactic acid bacteria were isolated from Zha-chili, which were identified as 15 species belonging to 8 genera.Among them, the dominant isolates were Companilactobacillus.alimentarius, Lactiplantibacillus.plantarum, and Lactiplantibacillus.pentosus, accounting for 23.73%, 22.03%, and 11.86% of the total isolates, respectively.From this, it can be seen that the Zha-chili from Zunyi region contains abundant resources of lactic acid bacteria and has high species diversity.

Key words： Zha-chili; PacBio SMRT sequencing technology; pure cultivation technology; bacterial diversity; lactic acid bacteria

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