综述与专题评论

基于纳米抗体的食源性致病菌快速检测方法研究进展

  • 张家豪 ,
  • 包雨宁 ,
  • 王妍入
展开
  • (西北农林科技大学 食品科学与工程学院,陕西 咸阳,712100)
第一作者:本科生(王妍入副教授为通信作者,E-mail:yanruwang22@163.com)

收稿日期: 2025-04-01

  修回日期: 2025-06-03

  网络出版日期: 2026-01-12

基金资助

国家自然科学基金面上项目(32472439);大学生创新创业项目(X202410712573)

Research progress of nanobody-based rapid detection methods for foodborne pathogens

  • ZHANG Jiahao ,
  • BAO Yuning ,
  • WANG Yanru
Expand
  • (College of Food Science and Engineering, Northwest A&F University, Xianyang 712100, China)

Received date: 2025-04-01

  Revised date: 2025-06-03

  Online published: 2026-01-12

摘要

食源性致病菌对食品安全领域造成了严重威胁,对人类生命健康带来了极为不利的影响。随着食品工业的迅速发展以及食品快速检测技术的不断完善,食源性致病菌的免疫分析技术成为了当下食品快速检测领域的热门课题。纳米抗体作为能与抗原特异性结合的最小抗体类型,相较于普通抗体具有亲和力强、灵敏度高、稳定性好、易于大批量生产等优点,在食品安全领域展现出巨大潜力,并且已经广泛应用于检测各类食源性危害物。该文通过介绍纳米抗体的结构及理化性质,综述了纳米抗体在食源性致病菌快速检测领域的最新研究进展,并归纳了该领域的不同创新性技术的特点,旨在为纳米抗体在食源性致病菌中的应用研究提供理论支持和实践指导。

本文引用格式

张家豪 , 包雨宁 , 王妍入 . 基于纳米抗体的食源性致病菌快速检测方法研究进展[J]. 食品与发酵工业, 2025 , 51(24) : 397 -408 . DOI: 10.13995/j.cnki.11-1802/ts.042907

Abstract

Foodborne pathogens pose a severe threat to the field of food safety and exert extremely adverse effects on human life and health.With the rapid development of the food industry and the continuous improvement of rapid food detection technologies, immunoassays for foodborne pathogens have become a hot topic in the field of food rapid detection.Nanobodies, as the smallest type of antibodies capable of specific binding to antigens, exhibit numerous advantages compared to conventional antibodies, including strong affinity, high sensitivity, good stability, and ease of large-scale production.They have been widely applied in the detection of various foodborne hazards, demonstrating immense potential in the field of food safety.This article introduces the structures and physicochemical properties of nanobodies, reviews the latest research progress in the rapid detection of foodborne pathogenic bacteria using nanobodies, and finally summarizes the characteristics of different innovative technologies in this field.The aim is to provide theoretical support and practical guidance for the application of nanobodies in rapid detection of foodborne pathogen.

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