[1] 李伊涵. 浅谈微生物食源性疾病现状及建议[J].食品界, 2024(9):93-95.
LI Y H.A brief discussion on the current situation of microbial foodborne diseases and suggestions[J].Food Industry, 2024(9):93-95.
[2] 陈秀琴, 黄梅清, 郑敏, 等.食源性致病菌快速检测技术及其应用研究进展[C].中国畜牧兽医学会兽医公共卫生学分会第六次学术研讨会论文集, 2018.
CHEN X Q, HUANG M Q, ZHENG M, et al.Advances on rapid detection of foodborne pathogens and the application[C].Collection of Papers from the Sixth Academic Seminar of the Veterinary Public Health Branch of Chinese Association of Animal Science and Veterinary Medicine, 2018.
[3] 王筱. 食源性致病菌:餐桌上的隐形威胁[J].家庭医学, 2025(5): 35-36.
WANG X. Foodborne pathogens: The invisible threat at the dinner table[J].Family Medicine, 2025(5): 35-36.
[4] 吕珍. 食品中食源性致病菌的检测与防控技术研究[J].中外食品工业, 2024(19):49-51.
LYU Z.Research on detection and prevention technologies for foodborne pathogens in food[J].Global Food Industry, 2024(19):49-51.
[5] World Health Organization.WHO estimates of the global burden of foodborne diseases[R].World Health Organization, 2015.
[6] 张翔渝. 食品安全快速检测技术在食源性致病菌检测中的应用[J].现代食品, 2024, 30(14):79-81.
ZHANG X Y.Application of rapid food safety detection technology in the detection of foodborne pathogens[J].Modern Food, 2024, 30(14):79-81.
[7] CONROY P J, LAW R H P, CARADOC-DAVIES T T, et al.Antibodies:From novel repertoires to defining and refining the structure of biologically important targets[J].Methods, 2017, 116:12-22.
[8] HAMERS-CASTERMAN C, ATARHOUCH T, MUYLDERMANS S, et al.Naturally occurring antibodies devoid of light chains[J].Nature, 1993, 363(6428):446-448.
[9] TANG H P, GAO Y, HAN J Y.Application progress of the single domain antibody in medicine[J].International Journal of Molecular Sciences, 2023, 24(4):4176.
[10] 刘星, 陈奇.鲨源单域抗体的研究进展[J].生物工程学报, 2020, 36(6):1069-1082.
LIU X, CHEN Q.Progress in shark single-domain antibody[J].Chinese Journal of Biotechnology, 2020, 36(6):1069-1082.
[11] NTANIOS F, MEIJER G, HEPBURN P.Comments on the review by Nguyen et al.The cholesterol-lowering action of plant stanol esters[J].The Journal of Nutrition, 2000, 130(9):2390-2392.
[12] 白梦凡. 基于纳米抗体磁富集的肠炎沙门氏菌免疫分析方法研究[D].杨凌:西北农林科技大学, 2022.
BAI M F.Development of a nanobody-based immunomagnetic separation-ELISA for rapid detection of Salmonella enteritidis in food[D].Yangling:Northwest A&F University, 2022.
[13] JOVČEVSKA I, MUYLDERMANS S.The therapeutic potential of nanobodies[J].BioDrugs, 2020, 34(1):11-26.
[14] NAMBULLI S, XIANG Y F, TILSTON-LUNEL N L, et al.Inhalable Nanobody (PiN-21) prevents and treats SARS-CoV-2 infections in Syrian hamsters at ultra-low doses[J].Science Advances, 2021, 7(22):eabh0319.
[15] MAEDA R, FUJITA J, KONISHI Y, et al.A panel of nanobodies recognizing conserved hidden clefts of all SARS-CoV-2 spike variants including omicron[J].Communications Biology, 2022, 5:669.
[16] HOU J, DU K J, LI J L, et al.Research trends in the use of nanobodies for cancer therapy[J].Journal of Controlled Release, 2025, 381:113454.
[17] 张翠. 鼠伤寒沙门氏菌纳米抗体的制备及免疫分析方法研究[D].杨凌:西北农林科技大学, 2022.
ZHANG C.The development of nanobody and construction of immunoassay towords Salmonella typhimurium[D].Yangling:Northwest A&F University, 2022.
[18] LAI J Y, LIM T S.Infectious disease antibodies for biomedical applications:A mini review of immune antibody phage library repertoire[J].International Journal of Biological Macromolecules, 2020, 163:640-648.
[19] 刘碧霞, 刘媛, 谢静, 等.噬菌体展示技术在全人源性抗体发现中的应用[J].免疫学杂志, 2023, 39(10):910-915.
LIU B X, LIU Y, XIE J, et al.Progresses of phage display technology application in fully human antibody discovery[J].Immunological Journal, 2023, 39(10):910-915.
[20] 唐秀兰, 邓安琦, 陈文聪, 等.纳米抗体筛选技术研究进展[J].生物工程学报, 2024, 40(2):350-366.
TANG X L, DENG A Q, CHEN W C, et al.Advances in nanobody screening technology[J].Chinese Journal of Biotechnology, 2024, 40(2):350-366.
[21] XU C X, YANG Y, LIU L W, et al.Microcystin-LR nanobody screening from an alpaca phage display nanobody library and its expression and application[J].Ecotoxicology and Environmental Safety, 2018, 151:220-227.
[22] PANG J R, GUO M W, WANG Y, et al.Determination of parathion by time-resolved fluorescence immunochromatographic assay based on nanobody:Aiming at improving strip sensitivity[J].Talanta, 2025, 285:127359.
[23] HE Q Q, CHEN Y J, WANG Z X, et al.Efficient selection of the 2,4-dichlorophenoxyacetic acid nanobody gene from the phage library constructed with sorted specific cells and expression in plants to confer herbicide resistance[J].Journal of Agricultural and Food Chemistry, 2024, 72(50):27850-27860.
[24] LIANG Y F, PAN Q Y, WANG Y, et al.Enhanced nanobody-driven bioluminescent immunoassay for rapid parathion detection using engineered split-nanoluciferase[J].Biosensors and Bioelectronics, 2025, 269:116913.
[25] ZHANG Y L, HE Z Y, SUN Z C, et al.Genetic engineering-powered dual-mode lateral flow immunosensor for colorimetric and fluorescent detection of ochratoxin A in pepper[J].Journal of Hazard Material, 2025, 489:137636.
[26] CAI C, XIA Y H, GUO Y Z, et al.Biosynthetic small molecule antigens mimics medicated lateral flow immunoassay for mycotoxin Fumonisin B1 using nanobody fusion proteins[J].Journal of Hazardous Materials, 2025, 487:137194.
[27] YUAN B, LI Z Q, LI P W, et al.Genetically engineered integrated aflatoxin B1 and deoxynivalenol bispecific nanobody as surrogate antigens for constructed time-resolved immunoassay dual detection methods[J].Biosensors and Bioelectronics, 2025, 273:117137.
[28] WANG Y, WU S H, WANG H T, et al.M13 bacteriophage based fluorescence immunoassay against food allergens of Ara h 3 and Mac i 1[J].Food Chemistry, 2025, 469:142617.
[29] BAN B, BLAKEII R C, BLAKE D A.Yeast surface display platform for rapid selection of an antibody library via sequential counter antigen flow cytometry[J].Antibodies, 2022, 11(4):61.
[30] DONG C, QIAO J, WANG X P, et al.Engineering Pichia pastoris with surface-display minicellulosomes for carboxymethyl cellulose hydrolysis and ethanol production[J].Biotechnology for Biofuels, 2020, 13(1):108.
[31] MÜLLER G A.Membrane insertion and intercellular transfer of glycosylphosphatidylinositol-anchored proteins:Potential therapeutic applications[J].Archives of Physiology and Biochemistry, 2020, 126(2):139-156.
[32] LI Y B, WANG X, ZHOU N Y, et al.Yeast surface display technology:Mechanisms, applications, and perspectives[J].Biotechnology Advances, 2024, 76:108422.
[33] 董新莹, 高晓薇, 宋浩, 等.纳米抗体的研究进展及其应用现状[J].生物工程学报, 2024, 40(12):4324-4338.
DONG X Y, GAO X W, SONG H, et al.Research progress and application of nanobodies[J].Chinese Journal of Biotechnology, 2024, 40(12):4324-4338.
[34] MEI M, LU M Q, LI S Q, et al.Development of nanobodies specific to clumping factors A of Staphylococcus aureus by yeast surface display[J].International Journal of Biological Macromolecules, 2024, 259:129208.
[35] 周永飞, 杨敬鹏, 常军亮, 等.人源化单克隆抗体的研究进展[J].中国生物制品学杂志, 2021, 34(9):1114-1119.
ZHOU Y F, YANG J P, CHANG J L, et al.Progress in research on humanized monoclonal antibody[J].Chinese Journal of Bioproducts, 2021, 34(9):1114-1119.
[36] 朱光, 王译晨, 宋莎莎, 等.纳米抗体筛选和表达技术研究进展[J].中国动物检疫, 2021, 38(7):79-87.
ZHU G, WANG Y C, SONG S S, et al.Research progress on the screening and expression of nanobody[J].China Animal Health Inspection, 2021, 38(7):79-87.
[37] WANG H, LIU R H.Advantages of mRNA display selections over other selection techniques for investigation of protein-protein interactions[J].Expert Review of Proteomics, 2011, 8(3):335-346.
[38] KANG Y L, CHEN L.Structural basis for the binding of DNP and purine nucleotides onto UCP1[J].Nature, 2023, 620(7972):226-231.
[39] BAKHERAD H, MOUSAVI GARGARI S L, RASOOLI I, et al.In vivo neutralization of botulinum neurotoxins serotype E with heavy-chain camelid antibodies (VHH)[J].Molecular Biotechnology, 2013, 55(2):159-167.
[40] BILLEN B, VINCKE C, HANSEN R, et al.Cytoplasmic versus periplasmic expression of site-specifically and bioorthogonally functionalized nanobodies using expressed protein ligation[J].Protein Expression and Purification, 2017, 133:25-34.
[41] YANG Z Y, SCHMIDT D, LIU W L, et al.A novel multivalent, single-domain antibody targeting TcdA and TcdB prevents fulminant Clostridium difficile infection in mice[J].The Journal of Infectious Diseases, 2014, 210(6):964-972.
[42] JÄRVILUOMA A, STRANDIN T, LÜLF S, et al.High-affinity target binding engineered via fusion of a single-domain antibody fragment with a ligand-tailored SH3 domain[J].PLoS One, 2012, 7(7):e40331.
[43] HENRY K A, SULEA T, VAN FAASSEN H, et al.A rational engineering strategy for designing protein A-binding camelid single-domain antibodies[J].PLoS One, 2016, 11(9):e0163113.
[44] OLICHON A, SURREY T.Selection of genetically encoded fluorescent single domain antibodies engineered for efficient expression in Escherichia coli[J].The Journal of Biological Chemistry, 2007, 282(50):36314-36320.
[45] BAGHBAN R, GARGARI S L M, RAJABIBAZL M, et al.Camelid-derived heavy-chain nanobody against Clostridium botulinum neurotoxin E in Pichia pastoris[J].Biotechnology and Applied Biochemistry, 2016, 63(2):200-205.
[46] BAGHBAN R, FARAJNIA S, GHASEMI Y, et al.New developments in Pichia pastoris expression system, review and update[J].Current Pharmaceutical Biotechnology, 2018, 19(6):451-467.
[47] FRENKEN L G J, VAN DER LINDEN R H J, HERMANS P W J, et al.Isolation of antigen specific Llama VHH antibody fragments and their high level secretion by Saccharomyces cerevisiae[J].Journal of Biotechnology, 2000, 78(1):11-21.
[48] 蔡美娜, 王佑春.哺乳动物细胞表达系统研究进展[J].中国医药生物技术, 2024, 19(3):254-259.
CAI M N, WANG Y C.Research progress on mammalian cell expression systems[J].Chinese Medicinal Biotechnology, 2024, 19(3):254-259.
[49] YOU M, LIU Y N, CHEN Y W, et al.Maximizing antibody production in suspension-cultured mammalian cells by the customized transient gene expression method[J].Bioscience, Biotechnology, and Biochemistry, 2013, 77(6):1207-1213.
[50] VANMARSENILLE C, ELSEVIERS J, YVANOFF C, et al.In planta expression of nanobody-based designer chicken antibodies targeting Campylobacter[J].PLoS One, 2018, 13(9):e0204222.
[51] HE Y X, REN Y R, GUO B, et al.Development of a specific nanobody and its application in rapid and selective determination of Salmonella enteritidis in milk[J].Food Chemistry, 2020, 310:125942.
[52] ZHANG C, LIU Z L, BAI M F, et al.An ultrasensitive sandwich chemiluminescent enzyme immunoassay based on phage-mediated double-nanobody for detection of Salmonella Typhimurium in food[J].Sensors and Actuators B: Chemical, 2022, 352:131058.
[53] REN Y R, WEI J, WANG Y, et al.Development of a streptavidin-bridged enhanced sandwich ELISA based on self-paired nanobodies for monitoring multiplex Salmonella serogroups[J].Analytica Chimica Acta, 2022, 1203:339705.
[54] WANG Y Q, ZHANG L, WANG P, et al.Enhancing oriented immobilization efficiency:A one-for-two organism-bispecific nanobody scaffold for highly sensitive detection of foodborne pathogens[J].Analytical Chemistry, 2023, 95(46):17135-17142.
[55] LIAO X R, WANG J M, GUO B, et al.Enhancing nanobody immunoassays through ferritin fusion:Construction of a Salmonella-specific fenobody for improved avidity and sensitivity[J].Journal of Agricultural and Food Chemistry, 2024, 72(26):14967-14974.
[56] BAI M F, WANG Y Q, ZHANG C, et al.Nanobody-based immunomagnetic separation platform for rapid isolation and detection of Salmonella enteritidis in food samples[J].Food Chemistry, 2023, 424:136416.
[57] ZHANG C, WANG Y Q, LIU Z L, et al.Nanobody-based immunochromatographic biosensor for colorimetric and photothermal dual-mode detection of foodborne pathogens[J].Sensors and Actuators B:Chemical, 2022, 369:132371.
[58] 孙颖. 金葡特异性纳米抗体的制备及免疫检测方法的构建[D].天津:天津科技大学, 2021.
SUN Y.Preparation of specific nanobodies against staphylococcus aureus and establishment of immunoassay[D].Tianjin:Tianjin University of Science and Technology, 2021.
[59] 唐丽. 金黄色葡萄球菌肠毒素B纳米抗体的制备及检测应用[D].赣州:赣南医科大学, 2024.
TANG L.Preparation and detection applications of staphylococcal enterotoxin B nanobodies[D].Ganzhou:Gannan Medical University, 2024.
[60] 郭鹏利, 路云龙, 李想, 等.基于纳米抗体的酶联免疫吸附法检测食品中金黄色葡萄球菌肠毒素B[J].食品与发酵工业, 2019, 45(20):250-255.
GUO P L, LU Y L, LI X, et al.Detection of Staphylococcal enterotoxin B in foodstuffs by nanobody-based ELISA[J].Food and Fermentation Industries, 2019, 45(20):250-255.
[61] WU H F, LI Y H, LI Y C, et al.The “umbrella of tolerance”:Nanobodies-armed photothermal lateral flow immunoassay for the detection of staphylococcal enterotoxin B[J].Chemical Engineering Journal, 2023, 470:144273.
[62] ZHANG Y, LIAO X R, YU G G, et al.Phage-displayed nanobody as a sensitive nanoprobe to enhance chemiluminescent immunoassay for Cronobacter sakazakii detection in dairy products[J].Analytical Chemistry, 2023, 95(36):13698-13707.
[63] CHEN P Y, YANG Q K, LI S R, et al.Nanobody-induced aggregation of gold nanoparticles:a mix-and-read strategy for the rapid detection of Cronobacter sakazakii[J].Analytical Chemistry, 2024, 96(44):17602-17611.
[64] HE Q Y, PAN J K, XU Z H, et al.Development of a nanobody-based immunoassay for the detection of Escherichia coli O157:H7 in food samples[J].Food Chemistry, 2025, 473:142987.
[65] DHEHIBI A, ALLAOUI A, RAOUAFI A, et al.Nanobody-based sandwich immunoassay for pathogenic Escherichia coli F17 strain detection[J].Biosensors, 2023, 13(2):299.
[66] WANG P, YU G G, WEI J, et al.A single thiolated-phage displayed nanobody-based biosensor for label-free detection of foodborne pathogen[J].Journal of Hazardous Materials, 2023, 443:130157.
[67] 王妍入, 魏娟, 王悦琦, 等.特异识别单增李斯特菌的纳米抗体, 重组载体, 宿主细胞及其应用:中国, CN202111407056.5[P].2024-10-30.
WANG Y R, WEI J, WANG Y Q, et al.Nanobodies specifically recognizing Listeria monocytogenes, recombinant vectors, host cells and their applications:China, CN202111407056.5[P].2024-10-30.
[68] TU Z, CHEN Q, Li Y P, et al.Identification and characterization of species-specific nanobodies for the detection of Listeria monocytogenes in milk[J].Analytical Biochemistry, 2016, 493:1-7.
[69] 周芳, 黄曾, 詹卓蓬, 等.一起沙门菌引起的食物中毒情况分析[J].实用临床医学, 2023, 24(1):99-101.
ZHOU F, HUANG Z, ZHAN Z C, et al.Analysis of a food poisoning incident caused by Salmonella[J].Practical Clinical Medicine, 2023, 24(1):99-101.
[70] 王庆信, 毛心怡, 时国庆.基于定向固定化抗体的侧向流免疫检测试纸建立食用油中玉米赤霉烯酮的检测方法[J].食品安全质量检测学报, 2024, 15(10):56-63.
WANG Q X, MAO X Y, SHI G Q.Development of lateral flow immunoassay based on oriented immobilized antibodies for the detection of zearalenone in edible oil[J].Journal of Food Safety and Quality, 2024, 15(10):56-63.
[71] 刘爱平, 申文浩, 王小红, 等.免疫学检测中抗体固定化方法的研究现状[J].江苏农业学报, 2017, 33(3):714-720.
LIU A P, SHEN W H, WANG X H, et al.A review on antibody immobilization in immunoassays[J].Jiangsu Journal of Agricultural Sciences, 2017, 33(3):714-720.
[72] AYRTON J P, HO C, ZHANG H R, et al.Multivalent nanobody engineering for enhanced physisorption and functional display on gold nanoparticles[J].Nanoscale, 2024, 16(42):19881-19896.
[73] SUN M X, SUN Y, YANG Y B, et al.Multivalent nanobody-based sandwich enzyme-linked immunosorbent assay for sensitive detection of porcine reproductive and respiratory syndrome virus[J].International Journal of Biological Macromolecules, 2024, 258:128896.
[74] 苑懿, 黄羽文, 邢巾, 等.基于多孔金@铂纳米酶的比色型生物传感器用于检测牛乳中鼠伤寒沙门氏菌研究[J].中国乳品工业, 2023, 51(12):48-53.
YUAN Y, HUANG Y W, XING J, et al.Colorimetric biosensor based on porous gold@platinum nanocatalysts for detection of Salmonella typhimurium in milk[J].China Dairy Industry, 2023, 51(12):48-53.
[75] 安刚. 食品中金黄色葡萄球菌肠毒素研究进展[J].食品安全导刊, 2024(33):141-143.
AN G.Research progress on staphylococcal enterotoxins in food[J].China Food Safety Magazine, 2024(33):141-143.
[76] ZHANG Y, LIU D, TIAN Y D, et al.Bifunctional nanobody facilitates a colorimetric and fluorescent dual-mode immunoassay of Staphylococcal enterotoxin A[J].Food Chemistry, 2025, 467:142362.
[77] 季艳伟, 崔艳, 张开惠, 等.特异性识别金黄色葡萄球菌肠毒素B、C的纳米抗体BC16及其应用:中国, CN202211032221.8[P].2023-08-11.
JI Y W, CUI Y, ZHANG K H, et al.Nanobody BC16 specifically recognizing Staphylococcus aureus enterotoxins B and C and its applications:China, CN202211032221.8[P].2023-08-11.
[78] HUGHES A C, KIRKLAND M, DU W X, et al.Development of thermally stable nanobodies for detection and neutralization of staphylococcal enterotoxin B[J].Toxins, 2023, 15(6):400.
[79] SONG D, CHENG J Q, DONG K, et al.Based on TLR4-NLRP3-IL-1β inflammatory pathway:Comparison of necrotizing enterocolitis induced by different classes of antibiotic-induced Cronobacter sakazakii[J].Food Bioscience, 2024, 62:105114.
[80] 张盼. 牛奶中大肠杆菌检测方法探究[J].食品安全导刊, 2025(5):148-150.
ZHANG P.Exploration on the detection methods of Escherichia coli in milk[J].China Food Safety Magazine, 2025(5):148-150.
[81] LIN J T, GU Y, XU Y R, et al.Characterization and applications of nanobodies against Pseudomonas aeruginosa Exotoxin A selected from single alpaca B cells[J].Biotechnology & Biotechnological Equipment, 2020, 34(1):1028-1037.
[82] STIJLEMANS B, CALJON G, NATESAN S K A, et al.High affinity nanobodies against the Trypanosome brucei VSG are potent trypanolytic agents that block endocytosis[J].PLoS Pathogens, 2011, 7(6):e1002072.
[83] WANG J, MUKHTAR H, MA L, et al.VHH antibodies:Reagents for mycotoxin detection in food products[J].Sensors, 2018, 18(2):485.
[84] BENNETT N R, WATSON J L, RAGOTTE R J, et al.Atomically accurate de novo design of antibodies with RFdiffusion[J].Nature, 2025:1-11.
[85] WANG Y D, XIANY Y L.Nanobody and nanozyme-enabled immunoassays with enhanced specificity and sensitivity[J].Small Methods, 2022, 6(4):2101576.