目前微生物发酵丙酸存在周期长,丙酸产量和纯度低等问题。为了获得高产丙酸的产酸丙酸杆菌,该文以微波-超声联合诱变,复合常温常压等离子体(atmospheric room temperature plasma, ARTP)诱变手段,对产酸丙酸杆菌PA8进行复合诱变以获得高产丙酸菌株。出发菌株PA8经微波100 s和超声9 s联合诱变筛选获得产丙酸提高14.54%的菌株LH54,随后以LH54为出发菌株进行ARTP诱变30 s,筛选获得高产突变株DLZ15。进一步采用正交试验优化了DLZ15的发酵培养基配比,经10 L罐发酵验证,150 h丙酸产量达到42.49 g/L,发酵强度0.297 g/(L·h),较初始出发菌株PA8提高48.50%;总发酵时间相较缩短42 h。DLZ15菌株及其配套发酵工艺有较好的工业发酵潜力。
At present, microbial fermentation of propionic acid faces issues such as long fermentation time, low yields, and low purity of propionic acid.To obtain a high propionic acid - producing Propionibibacterium acidipropionici strain, this study employs a combination of microwave-ultrasonic combined mutagenesis and atmospheric room temperature plasma (ARTP) mutagenesis to conduct compound mutagenesis on the initial strain Propionibacterium acidipropionici PA8 was subjected to microwave treatment for 100 s and ultrasonic treatment for 9 s.After this combined mutagenesis, the strain LH54 was selected, which exhibiting a 14.54% higher propionic acid yield than PA8.Subsequently, using LH54 as the starting strain, ARTP mutagenesis was performed for 30 s, and the high-yielding mutant strain DLZ15 was selected.Further optimization of the fermentation medium composition for DLZ15 was carried out using orthogonal experiments.Verification through 10 L fermentation revealed that after 150 hours, the propionic acid yield reached 42.49 g/L, with a fermentation intensity of 0.297 g/(L·h).Compared with the initial starting strain PA8, this represents an increase of 48.50%.Moreover, the total fermentation time was reduced by 42 hours.The strain DLZ15 and its optimized fermentation medium components hold significant value for industrial fermentation.
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