食品与发酵工业 >

2026 , Vol. 52 >Issue 14: 343 - 350

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.044605

分析与检测

离子色谱法同时测定发酵液中22种糖醇和糖类

  • 江黎黎 ,
  • 韩梅 ,
  • 陆万平 ,
  • 陈久耿
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  • 1(上海交通大学 分析测试中心,上海, 200240)
    2(上海商学院,食品质量与安全,上海, 200235)
第一作者:博士研究生,实验师(陈久耿实验师为通信作者,E-mail:jiugengchen@sjtu.edu.cn)

收稿日期: 2025-09-22

  修回日期: 2025-12-22

  网络出版日期: 2026-08-21

基金资助

上海市白玉兰人才计划浦江项目(24PJD081)

收起

Simultaneous determination of 22 sugar alcohols and sugar in fermentation broth by ion chromatography

  • JIANG Lili ,
  • HAN Mei ,
  • LU Wanpin ,
  • CHEN Jiugeng
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  • 1(Shanghai Jjiaotong University, Instrumental Analysis Center, Shanghai 200240, China)
    2(Shanghai Business School, Food Quality and Safety, Shanghai 200235, China)

Received date: 2025-09-22

  Revised date: 2025-12-22

  Online published: 2026-08-21

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摘要

该文建立了一种可同时测定单糖、双糖、糖醇及糖醛酸等多种糖类且高灵敏度的离子色谱-安培检测器方法,用于表征微生物在复杂组合碳源体系下对不同碳源的利用顺序。通过研究色谱柱类型、淋洗浓度、色谱柱温度和流速等因素,在55 min内同时测定22种糖组分含量。实验结果显示,在流速为0.45 mL/min,采用氢氧化钠和乙酸钠的梯度淋洗,且柱温设定为30 ℃时,这22种组分在0.1~10 mg/L的质量浓度范围内展现出良好的线性关系(r2＞0.995),这些组分的检出限为0.005~0.098 mg/L。在0.5、2.0、4.0 mg/L这3个浓度水平下,22种组分的平均回收率为77%~110%。该方法具有高效、灵敏和准确的特点,可将该方法初步应用于考察嗜热链球菌对不同类型碳源的偏好性实验。

关键词： 离子色谱法; 发酵液; 糖醇; 单糖; 寡糖; 含量

本文引用格式

江黎黎 , 韩梅 , 陆万平 , 陈久耿 . 离子色谱法同时测定发酵液中22种糖醇和糖类[J]. 食品与发酵工业, 2026 , 52(14) : 343 -350 . DOI: 10.13995/j.cnki.11-1802/ts.044605

Abstract

This paper has established an ion chromatography (IC)-pulsed amperometric detector (PAD) method with high sensitivity for simultaneously determining various sugars such as monosaccharides, disaccharides, sugar alcohols and uronic acid, which can be used to characterize the utilization sequence of different carbon sources by microorganisms in a complex combination of carbon sources.Through the study of factors such as column type, eluent concentration, column temperature and flow rate, an analytical method for the simultaneous determination of the contents of 22 sugar components within 55 minutes was established.in fermentation.By investigating the key parameters such as elution concentration, column temperature, and flow rate, we established an analytical method capable of quantifying all 22 components within 55 minutes.Experimental results demonstrated that at a flow rate of 0.45 mL/min, using gradient elution with sodium hydroxide and sodium acetate, and maintaining a column temperature of 30 ℃, all 22 components exhibited good linearity (r2>0.995) within the concentration range of 0.1 to 10 mg/L.The limits of detection (LODs) for these components ranged from 0.005 to 0.098 mg/L.At three concentration levels of 0.5 mg/L, 2.0 mg/L, and 4.0 mg/L, the average recovery rates for the 22 components ranged from 77% to 110%.This method demonstrates high efficiency, sensitivity, and accuracy and has been applied to the investigate the preference of Streptococcus thermophilus for different types of carbon sources.

Key words： ion chromatography; zymotic fluid; sugar alcohols; monosaccharide; oligosaccharide; content

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