Additives and Contaminants, 2006,23(11):1 056-1 063.Separation, structure identification and content analysis of different forms of astaxanthin in Antarctic krill
CONG Xinyuan1, SUN Weihong2*, ZHANG Huizhen3*, LENG Kailiang2, XING Lihong2, MIAO Junkui2,4, LIU Xiaofang2,4
1(Qingdao University,School of Public Health,Qingdao 266071, China); 2(Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Polar Fishery, Ministry of Agriculture, Qingdao 266071, China); 3(Qingdao Food and Drug Administration, Qingdao 266071, China); 4(Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology,Qingdao 266071, China)
Abstract: In this study, acetone was used to extract the astaxanthin from Antarctic krill. The silica gel column chromatography was optimised for separating astaxanthin diester, astaxanthin monoester, and free astaxanthin. A suitable developing solvent was selected. Samples were identified by a thin layer chromatography and the three-dimensional structures were confirmed by Chiralpak IC column. The contents of astaxanthin in different forms were quantified using C30-HPLC. The results showed that a gradient elution through silica gel column chromatography with different ratios of acetone-petroleum ether could completely separate free astaxanthin, astaxanthin monoester and diester. V (n-hexane)∶V (acetone)∶V (acetic acid)=8∶2∶0.2 was the optimum developing solvent for thin layer chromatography. Astaxanthin from Antarctic krill included three optical isomers: 3S,3′S, 3S,3′R, and 3R,3′R. The proportions of 3R,3′R in astaxanthin diesters and monoesters were more than 70%, and the proportion of 3S,3′S was the lowest. The total astaxanthin content in Antarctic krill was 18.9 mg/kg, of which the astaxanthin contents of astaxanthin diester, astaxanthin monoester, and free astaxanthin were 12.0, 6.1 and 0.8 mg/kg, respectively.
丛心缘,孙伟红,张辉珍,等. 南极磷虾中不同形态虾青素的分离制备、结构鉴定及含量分析[J]. 食品与发酵工业, 2019, 45(2): 174-178.
CONG Xinyuan,SUN Weihong,ZHANG Huizhen,et al. Additives and Contaminants, 2006,23(11):1 056-1 063.Separation, structure identification and content analysis of different forms of astaxanthin in Antarctic krill[J]. Food and Fermentation Industries, 2019, 45(2): 174-178.
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2019, Vol. 45 
