Abstract: Pesticide residues in foods and environment pose threats to human health, therefore, discovering new enzyme sources from plants can help reduce the cost of rapid pesticide detection by enzymatic inhibition method. Plant esterases from peas were purified, classified and characterized. Their pesticide sensitivities were also detected. The results showed that the purified pea esterase was about 26.0 kDa with high esterase activity, and it was classified as a carboxylesterase. Moreover, it demonstrated the best catalytic activity at 40 ℃ and pH 6.5 at 0.45 μg/mL. Furthermore, it could be inhibited by organophosphate and carbamate pesticides. In conclusion, pea esterase is a type B esterase and can be used to detect organophosphorus and carbamate pesticide residues. Besides, the purified pea esterase also meets the requirements of most pesticide MRL tests.
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2019, Vol. 45 
