生产与科研经验

南极磷虾中不同形态虾青素的分离制备、结构鉴定及含量分析

  • 丛心缘 ,
  • 孙伟红 ,
  • 张辉珍 ,
  • 冷凯良 ,
  • 邢丽红 ,
  • 苗钧魁 ,
  • 刘小芳
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  • 1(青岛大学 公共卫生学院,山东 青岛,266071);
    2(中国水产科学研究院黄海水产研究所,农业部极地渔业开发重点实验室,山东 青岛,266071);
    3(青岛市食品药品监督管理局,山东 青岛,266100);
    4(海洋国家实验室海洋药物与生物制品功能实验室,山东 青岛,266100)
硕士研究生(孙伟红博士和张辉珍博士为共同通讯作者,E-mail:sunwh@ysfri.ac.cn,13963988819@163.com)。

收稿日期: 2018-04-19

  网络出版日期: 2019-02-21

基金资助

中国水产科学研究院基本科研业务费专项(2016 HY-ZD0902)资助

Additives and Contaminants, 2006,23(11):1 056-1 063.Separation, structure identification and content analysis of different forms of astaxanthin in Antarctic krill

  • CONG Xinyuan ,
  • SUN Weihong ,
  • ZHANG Huizhen ,
  • LENG Kailiang ,
  • XING Lihong ,
  • MIAO Junkui ,
  • LIU Xiaofang
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  • 1(Qingdao University,School of Public Health,Qingdao 266071, China);
    2(Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Polar Fishery, Ministry of Agriculture, Qingdao 266071, China);
    3(Qingdao Food and Drug Administration, Qingdao 266071, China);
    4(Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology,Qingdao 266071, China)

Received date: 2018-04-19

  Online published: 2019-02-21

摘要

以南极磷虾为研究对象,采用丙酮提取南极磷虾中的虾青素。优化硅胶柱层析技术分离虾青素双酯、单酯及游离虾青素;筛选合适的展开剂,利用薄层层析法对得到的样品进行鉴定;通过Chiralpak IC柱鉴别其立体结构;运用C30-HPLC法测定不同形态虾青素的含量。结果表明,硅胶柱层析以不同比例的丙酮-石油醚溶液梯度淋洗,可以分离制备虾青素双酯、单酯及游离虾青素;V(正己烷)∶V(丙酮)∶V(乙酸)=8∶2∶0.2 为薄层层析最佳展开剂。南极磷虾中不同形态虾青素均由3S,3′S、3S,3′R、3R,3′R 3种光学异构体组成,虾青素双酯和单酯中3R,3′R所占比例>70%,3S,3′S的比例最低。南极磷虾中总虾青素含量为18.9 mg/kg,其中虾青素双酯中虾青素含量为12.0 mg/kg,虾青素单酯中虾青素含量为6.1 mg/kg,游离虾青素含量为0.8 mg/kg。

本文引用格式

丛心缘 , 孙伟红 , 张辉珍 , 冷凯良 , 邢丽红 , 苗钧魁 , 刘小芳 . 南极磷虾中不同形态虾青素的分离制备、结构鉴定及含量分析[J]. 食品与发酵工业, 2019 , 45(2) : 174 -178 . DOI: 10.13995/j.cnki.11-1802/ts.017586

Abstract

In this study, acetone was used to extract the astaxanthin from Antarctic krill. The silica gel column chromatography was optimised for separating astaxanthin diester, astaxanthin monoester, and free astaxanthin. A suitable developing solvent was selected. Samples were identified by a thin layer chromatography and the three-dimensional structures were confirmed by Chiralpak IC column. The contents of astaxanthin in different forms were quantified using C30-HPLC. The results showed that a gradient elution through silica gel column chromatography with different ratios of acetone-petroleum ether could completely separate free astaxanthin, astaxanthin monoester and diester. V (n-hexane)∶V (acetone)∶V (acetic acid)=8∶2∶0.2 was the optimum developing solvent for thin layer chromatography. Astaxanthin from Antarctic krill included three optical isomers: 3S,3′S, 3S,3′R, and 3R,3′R. The proportions of 3R,3′R in astaxanthin diesters and monoesters were more than 70%, and the proportion of 3S,3′S was the lowest. The total astaxanthin content in Antarctic krill was 18.9 mg/kg, of which the astaxanthin contents of astaxanthin diester, astaxanthin monoester, and free astaxanthin were 12.0, 6.1 and 0.8 mg/kg, respectively.

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