为了优化解淀粉芽孢杆菌产葡甘聚糖酶的条件,在单因素试验的基础上,利用响应面法分析发酵条件,并研究葡甘聚糖酶的酶学性质。结果表明,解淀粉芽孢杆菌产葡甘聚糖酶的最佳培养基成分为葡萄糖40 g/L、酵母粉9 g/L、硫酸铵0.8 g/L;最佳发酵条件为温度30 ℃、发酵时间80 h、接种量8%(体积分数)、装液量100 mL/250 mL, 在此条件得到的葡甘聚糖酶活力可达2 509 U/mL。该葡甘聚糖酶的最适反应条件为50 ℃、pH 7; 在30~40 ℃、pH 3~7酶活力稳定;Al3+、Fe3+、Cu2+等离子对葡甘聚糖酶有抑制作用,Mn2+有激活作用。
This study aimed to optimize the conditions to produce glucomannase by Bacillus amyloliquefaciens fermentation. The fermentation conditions were analyzed by response surface methodology based on single factor experiments. The results indicated that the activity of glucomannase was up to 2 509 U/mL under optimized culture medium and fermentation conditions (40 g/L glucose, 9 g/L yeast powder, 0.8 g/L ammonium sulfate, fermented at 30 ℃ for 80 h, with 8% inoculation size and 100 mL working volume in a 250 mL shaking flask). Glucomannase prepared under this condition had the maximum activity at 50 ℃ and at pH=7. It was relatively stable at 30~40 ℃ and at pH=3-7. Moreover, ions such as Al3+, Fe3+, and Cu2+ etc. inhibited its activity, whereas Mn2+ had activating effect.
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