采用Folin-Ciocalteu比色法和LC-DAD-ESI-MS法对陈年生普(以下简称APT)和熟普(以下简称YPT)中总多酚(The total phenolic, TPC)和黄烷醇类活性成分进行分析。研究结果表明,从APT和YPT中确定了8种黄烷醇类活性成分,它们分别是儿茶素(C)、表儿茶素(EC)、表儿茶素没食子酸酯(ECG)、表没食子儿茶素(EGC)、表没食子儿茶素没食子酸酯(EGCG)、没食子儿茶素(GC)、没食子儿茶素没食子酸酯(GCG)、儿茶素没食子酸酯(CG)。2种茶中GC含量差异不显著,APT中的TPC和其余7种黄烷醇类化合物含量均显著高于YPT中。不同产地普洱茶的TPC和8种黄烷醇类化合物含量差异不显著,不同年份差异显著。主成分分析法(PCA)和层次聚类分析(HCA)依据TPC和黄烷醇类化合物能将APT和YPT很好的聚类,成功区分出APT和YPT样品;除GC外的其余7种黄烷醇类化合物和TPC均是很好的化学标记。
The purpose of this study was to supervise the quality of Pu'er tea and provide reference for consumers to purchase Pu'er tea. Folin-ciocalteu colorimetry and LC-DAD-ESI-MS were used to analyze total polyphenols (TPC) and flavanols in aged raw Pu'er tea (APT) and young ripened Pu'er tea (YPT). The results showed that there were eight flavanols identified from APT and YPT, which were catechin (C), epicatechin (EC), epicatechin gallic acid ester (ECG), table gallic catechins (EGC), table gallic catechin gallic acid ester (EGCG), gallnut catechins (GC), gallnut catechin gallic acid ester (GCG), and catechin gallate (CG). There was no significant difference in GC content between two teas while the contents of TPC and other seven flavanols in APT were significantly higher than that in YPT. Moreover, the contents of TPC and flavanols in Pu'er tea from different locations were not significantly different, but differences between teas with different years were significant. Furthermore, principal component analysis (PCA) and hierarchical clustering analysis (HCA) clustered APT and YPT well and therefore they could be distinguished from each other. Overall, in addition to GC, the other seven flavanols and TPC are good chemical markers, and combination of PCA and HCA can be used as an appropriate method to distinguish APT from YPT.
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