食品与发酵工业 >

2019 , Vol. 45 >Issue 12: 67 - 74

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.019561

研究报告

卵白蛋白低聚肽的体外抗氧化及ACE抑制作用

  • 张江涛 ,
  • 秦修远 ,
  • 贾福怀 ,
  • 袁媛 ,
  • 周明 ,
  • 方磊 ,
  • 张瑞雪 ,
  • 谷瑞增 ,
  • 刘文颖
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  • 1.中国食品发酵工业研究院,北京市蛋白功能肽工程技术研究中心,北京,100015；
    2.宁波御坊堂生物科技有限公司,浙江 宁波,315012
张江涛(硕士)和秦修远(硕士)为共同第一作者(谷瑞增教授级高级工程师和刘文颖高级工程师为共同通讯作者,E-mail:guruizeng@126.com;wenyingliu888@126.com)。

收稿日期: 2018-12-07

  修回日期: 2019-01-14

  网络出版日期: 2019-07-16

基金资助

“十三五”国家重点研发计划(2016YFD0400604);国家自然科学基金(31671963);中山市协同创新中心认定资助项目(2016C1015);宁波市“十三五”海洋经济创新发展示范项目(NBHY-2017-S1)

收起

In vitro antioxidant and ACE inhibition effects of ovalbumin oligopeptides

  • ZHANG Jiangtao ,
  • QIN Xiuyuan ,
  • JIA Fuhuai ,
  • YUAN Yuan ,
  • ZHOU Ming ,
  • FANG Lei ,
  • ZHANG Ruixue ,
  • GU Ruizeng ,
  • LIU Wenying
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  • 1. China National Research Institute of Food & Fermentation Industry,Beijing Engineering Research Center of Protein and Functional Peptides,Beijing 100015, China;
    2. Ningbo Yufangtang Bio-tech Co.,Ltd.,Ningbo 315012, China

Received date: 2018-12-07

  Revised date: 2019-01-14

  Online published: 2019-07-16

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摘要

以卵白蛋白低聚肽为研究对象,测定了理化成分,分子质量分布和氨基酸组成基础之上;通过DPPH·法、邻苯三酚自氧化法和水杨酸钠法测定对DPPH自由基(DPPH·)、超氧阴离子自由基(O-2·)和羟自由基(·OH)的清除能力,分析了其体外抗氧化作用;另外通过反向高效液相色谱法测定了对血管紧张素转化酶(angiotensin converting enzyme,ACE)的抑制作用。结果显示,分子质量低于1 000 u的卵白蛋白低聚肽占总蛋白的92.58％,并主要集中在140~500 u(占71.24％);体外抗氧化实验中,抗氧化能力与质量浓度呈现出良好的剂量关系,DPPH·、O-2·和·OH的半抑制浓度(IC50)分别为0.25、25、0.64 mg/mL;另外卵白蛋白低聚肽显示出较好的ACE抑制作用,IC50值为1.5 mg/mL。结果表明,卵白蛋白低聚肽具有良好的抗氧化和ACE抑制作用,为相关抗氧化和降血压等保健类食品的开发利用提供一定的理论依据。

关键词： 卵白蛋白低聚肽; 氨基酸组成; 分子质量分布; 抗氧化; 血管紧张素转化酶

本文引用格式

张江涛 , 秦修远 , 贾福怀 , 袁媛 , 周明 , 方磊 , 张瑞雪 , 谷瑞增 , 刘文颖 . 卵白蛋白低聚肽的体外抗氧化及ACE抑制作用[J]. 食品与发酵工业, 2019 , 45(12) : 67 -74 . DOI: 10.13995/j.cnki.11-1802/ts.019561

Abstract

The ovalbumin oligopeptides were used as research object, and their physicochemical components, molecular weight distribution and amino acid composition were determined. In order to analyze their antioxidant activities in vitro, their abilities to scavenge DPPH free radicals, superoxide anion radicals and hydroxyl radicals were determined. In addition, their effects on inhibiting angiotensin converting enzyme (ACE) were analyzed by reverse high performance liquid chromatography. The results showed that the ovalbumin oligopeptides with a molecular weight of less than 1 000 u accounted for 92.58% of the total protein, and were mainly concentrated between 140 u and 500 u (71.24%). Moreover, their antioxidant capacities showed good dose relationships with their mass concentration. Their semi-inhibitory concentrations (IC50) of DPPH free radicals, superoxide anion radicals and hydroxyl radicals were 0.25 mg/mL, 25 mg/mL, and 0.64 mg/mL, respectively. Furthermore, ovalbumin oligopeptides showed better ACE inhibitory effects with an IC50 value of 1.5 mg/mL. The results showed that ovalbumin oligopeptides have good antioxidative and ACE inhibition effects, which provides a theoretical basis for developing and utilizing health-care foods with antioxidative and blood pressure lowering effects.

Key words： ovalbumin oligopeptides; amino acid composition; molecular mass distribution; antioxidant; angiotensin converting enzyme(ACE)

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